Application of large language models in disease diagnosis and treatment.

Large language models (LLMs) such as ChatGPT, Claude, Llama, and Qwen are emerging as transformative technologies for the diagnosis and treatment of various diseases. With their exceptional long-context reasoning capabilities, LLMs are proficient in clinically relevant tasks, particularly in medical text analysis and interactive dialogue. They can enhance diagnostic accuracy by processing vast amounts of patient data and medical literature and have demonstrated their utility in diagnosing common diseases and facilitating the identification of rare diseases by recognizing subtle patterns in symptoms and test results.

mRNA-miRNA analyses reveal the involvement of CsbHLH1 and miR1446a in the regulation of caffeine biosynthesis in .

Caffeine, a primary flavor component in tea, has been the subject of intense research. With the goal of shedding light on the complex regulatory processes governing caffeine biosynthesis in tea plants, liquid chromatography coupled with mass spectrometry (LC-MS), transcriptomics, and small RNA analyses were employed on diverse tea cultivars such as 'Jianghua Kucha' [including 'Xianghong 3' (XH3H) and 'Kucha 3' (KC3H)], 'Fuding Dabaicha' (FDDB), 'Yaoshan Xiulv' (YSXL), and 'Bixiangzao' (BXZ). The results showed that the caffeine level in 'Jianghua Kucha' was significantly higher than that in other tea plant cultivars.

Generating a 30 m Hourly Land Surface Temperatures Based on Spatial Fusion Model and Machine Learning Algorithm.

Land surface temperature (LST) is a critical parameter for understanding climate change and maintaining hydrological balance across local and global scales. However, existing satellite LST products face trade-offs between spatial and temporal resolutions, making it challenging to provide all-weather LST with high spatiotemporal resolution. In this study, focusing on Chengdu city, a framework combining a spatiotemporal fusion model and machine learning algorithm was proposed and applied to retrieve hourly high spatial resolution LST data from Chinese geostationary weather satellite data and multi-scale polar-orbiting satellite observations.

Associations between modifiable risk factors and cognitive function in middle-aged and older Chinese adults: joint modelling of longitudinal and survival data.

Background: Stronger associations between modifiable risk factors and cognitive function have been found in younger than older adults. This age pattern may be subject to mortality selection and non-ignorable missingness caused by dropouts due to death, but this remains unclear.

Methods: Longitudinal data from 9,562 adults aged 50 and older from Waves 1-4 (2011-2018) of the China Health and Retirement Longitudinal Study were used.

TRPC6 knockdown-mediated ERK1/2 inactivation alleviates podocyte injury in minimal change disease via upregulating Lon peptidase 1.

Minimal change disease (MCD) is a universal primary glomerular disease contributing to nephrotic syndrome. Lon peptidase 1 (LONP1) has been suggested to protect podocytes from damage during the progression of MCD. Accordingly, our research further explored the specific mechanisms of LONP1.

The antiviral effect and potential mechanism of Houttuynia cordata thunb. (HC) against coxsackievirus A4.

Ethnopharmacological Relevance: Hand, foot, and mouth disease (HFMD) is mainly caused by various of enteroviruses such as enterovirus 71 (EVA71), coxsackievirus A16 (CVA16), CVA6, and CVA10 in infants and children under 5 years old. During the past 5 years, CVA4 has become the dominant pathogen resulting in HFMD in China. However, there are no effective vaccines and antiviral drugs available.

Long-Term Efficacy of an AI-Based Health Coaching Mobile App in Slowing the Progression of Nondialysis-Dependent Chronic Kidney Disease: Retrospective Cohort Study.

Background: Chronic kidney disease (CKD) is a significant public health concern. Therefore, practical strategies for slowing CKD progression and improving patient outcomes are imperative. There is limited evidence to substantiate the efficacy of mobile app-based nursing systems for decelerating CKD progression.

Clinical efficacy of Fufang Yinhua Jiedu (FFYH) granules in mild COVID-19 and its anti-SARS-CoV-2 mechanism by blocking autophagy through inhibiting the AKT/mTOR signaling pathway.

Background: Fufang Yinhua Jiedu (FFYH) granules are recommended for treating coronavirus pneumonia (COVID-19) in China. However, its anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) activity and clinical efficacy against COVID-19 remain to be confirmed.

Aims: Our study aimed to investigate the anti-SARS-CoV-2 effect and potential mechanism of FFYH.

Synthesis and Biological Evaluation of Deoxycyclophellitols as Human Retaining β-Glucosidase Inhibitors.

Cyclophellitol is a potent and selective mechanism-based retaining β-glucosidase inhibitor that has served as a versatile starting point for the development of activity-based glycosidase probes (ABPs). We developed routes of synthesis of eight mono- and dideoxycyclophellitols and cyclophellitol aziridines, the latter as ABPs carrying either a biotin or fluorophore linked to the aziridine nitrogen. We reveal the potency of these 24 compounds as inhibitors of the three human retaining β-glucosidases, GBA1, GBA2 and GBA3.

Generalized dark hollow sine-Gaussian beam and its propagation properties.

A model of the generalized dark hollow sine-Gaussian beam (GDHsGB) is proposed to uniformly describe both conventional dark hollow beams (DHBs) and anomalous dark hollow beams (ADHBs) with circular or elliptic geometrical patterns. Using the Collins formula, we derive the analytical expression for GDHsGBs propagating in ABCD paraxial optical systems. We analyze the evolution of the intensity pattern and beam width of circular ADHBs, as well as the ellipticity of elliptic ADHBs, providing mathematical expressions for these physical quantities.

The therapeutic effect of Yinqiaosan decoction against influenza A virus infection by regulating T cell receptor signaling pathway.

Background: Yinqiaosan decoction (YQSD), a traditional Chinese medicinal recipe, has been employed to treat influenza in China for approximately 300 years.

Objective: Our study aimed to explore the mechanisms of YQSD against influenza via and experimental studies.

Study Design: and methods UHPLC-Q-TOF-MS/MS was utilized to examine the substances of the YQSD.

Using geotagged facial expressions to visualize and characterize different demographic groups' emotion in theme parks.

Tourism is an emotional sphere, and researchers focus on emotions to optimize tourism experiences. Tourism studies on emotions mostly ignore differences in emotions across demographic tourist groups by gender and age, thus limiting the understanding of emotions to the explicit characteristics of tourists' emotions. On the basis of geotagged facial expressions on social media platforms, this study aims to visualize the emotions of groups in scenic spots and then reveal the variations between groups' emotions within theme parks.

A daily exercise prescription when work gets tough: The moderating effect of work demands on the relationship between daily physical exercise and next-day well-being and job performance.

Physical exercise is widely recognized for its benefits to individuals' general health, yet its implications for in-role and extrarole job performance, especially on demanding workdays, have rarely been explored. This oversight is concerning as high work demands can deter employees from exercising when they are unaware that exercise can improve their job performance on demanding workdays. In this research, we draw on the effort-recovery model to propose that daily physical exercise not only promotes next-day well-being but also enhances next-day in-role job performance and extrarole organizational citizenship behavior (OCB) by fostering positive affect and work engagement the following day.

The AAV2.7m8 capsid packages a higher degree of heterogeneous vector genomes than AAV2.

Recombinant adeno-associated virus (rAAV) vectors are currently the only proven vehicles for treating ophthalmological diseases through gene therapy. A wide range of gene therapy programs that target ocular diseases are currently being pursued. Nearly 20 years of research have gone into enhancing the efficacy of targeting retinal tissues and improving transgene delivery to specific cell types.

Comparative Proteomics and Metabonomics Analysis of Different Diapause Stages Revealed a New Regulation Mechanism of Diapause in (Lepidoptera: Pyralidae).

Histone acetylation is an important epigenetic mechanism that has been shown to play a role in diapause regulation. To explore the physiological and molecular mechanisms of histone deacetylase in the diapause process, LC-MS/MS analysis was used to perform TMT proteomic and metabolomic analysis on non-diapause (ND), pre-diapause (PreD), diapause (D), cold treatment (CT), and post-diapause (RD) stages of the meadow moth. A total of 5367 proteins were identified by proteomics, including 1179 differentially expressed proteins.

Skin locations inference and body fluid identification from skin microbial patterns for forensic applications.

Given that microbiological analysis can be an alternative method that overcomes the shortcomings of traditional forensic technology, and skin samples may be the most common source of cases, the analysis of skin microbiome was investigated in this study. High-throughput sequencing targeting the V3-V4 region of 16S rRNA gene was performed to reveal the skin microbiome of healthy individuals in Guangdong Han. The bacterial diversity of the palm, navel, groin and plantar of the same individual was analyzed.

The efficacy of sacubitril/valsartan in patients receiving peritoneal dialysis with diabetes and heart failure with preserved ejection fraction.

Objective: To investigate the efficacy of sacubitril/valsartan in the treatment of peritoneal dialysis (PD) in patients with diabetes and heart failure with preserved ejection fraction (HFpEF).

Materials And Methods: Patients with diabetes who underwent PD and had HFpEF (n = 64) were divided into two groups: the experimental group (n = 31), which was administered sacubitril/valsartan, and the control group (n = 33), administered valsartan alone. Data were collected before and after treatment to compare the inter-group changes in cardiac function indexes, residual renal function (RRF), and PD adequacy indexes.

Circ-USP9X accelerates deep vein thrombosis after fracture by acting as a miR-148b-3p sponge and upregulates SRC kinase signaling inhibitor 1.

Objectives: This study aims to elucidate the role of circUSP9X (Circular RNA Ubiquitin Specific Peptidase 9 X-Linked) in the development of venous thrombosis in the lower extremities.

Methods: An animal model of Deep Vein Thrombosis (DVT) and a hypoxic model of Human Umbilical Vein Endothelial Cells (HUVECs) treated with Cobalt (II) Chloride (CoCl) were developed. The expression levels of circUSP9X, microRNA-148b-3p (miR-148b-3p), and SRC Kinase Signaling Inhibitor 1 (SRCIN1) were quantified using quantitative reverse transcription Polymerase Chain Reaction and Western blot analysis.