Integrated bioinformatics analysis and experimental validation of exosome-related gene signature in steroid-induced osteonecrosis of the femoral head.

Background: Steroid-induced osteonecrosis of the femoral head (SIONFH) is a universal hip articular disease and is very hard to perceive at an early stage. The understanding of the pathogenesis of SIONFH is still limited, and the identification of efficient diagnostic biomarkers is insufficient. This research aims to recognize and validate the latent exosome-related molecular signature in SIONFH diagnosis by employing bioinformatics to investigate exosome-related mechanisms in SIONFH.

Laennec approach for anatomical liver resection assisted by laparoscopy or robotics: a multicenter cohort study.

Introduction: Laennec's capsule serves as a critical anatomical landmark in anatomical liver resection. Despite its potential, a lack of large-scale prospective studies limits the widespread use of the Laennec approach for minimally invasive hepatectomy. This multicenter cohort study aimed to compare the outcomes of the traditional and Laennec approaches in minimally invasive anatomical hepatectomy across multiple centers in China.

Integration of untargeted lipidomics and targeted metabolomics revealed the mechanism of flavor formation in lightly cured sea bass driven via salt.

Salt enhances flavor and salinity in Chinese curing; however, excessive use can pose health risks, while reducing NaCl may harm taste. This study utilized targeted and untargeted metabolomics to investigate the intrinsic molecular mechanisms that drive flavor formation in cured sea bass subjected to salt. Glycine, succinic acid, lactic acid and uridine significantly contributed to the taste profile of the cured sea bass.

Localized In Vivo Prodrug Activation Using Radionuclides.

Radionuclides used for imaging and therapy can show high molecular specificity in the body with appropriate targeting ligands. We hypothesized that local energy delivered by molecularly targeted radionuclides could chemically activate prodrugs at disease sites while avoiding activation in off-target sites of toxicity. As proof of principle, we tested whether this strategy of radionuclide-induced drug engagement for release (RAiDER) could locally deliver combined radiation and chemotherapy to maximize tumor cytotoxicity while minimizing off-target exposure to activated chemotherapy.

Mechanism of differentiated and targeted catalysis in complex lipid system under lipase and lipoxygenase mediation.

The regulation of reaction rate differentiation, catalytic precursor differentiation, and end-product differentiation during enzyme-mediated reactions within complex lipid systems is a key area of research in flavor regulation. A multilayer lipid oxidation model, utilizing Plaice bone oil (PBO), lipase, and lipoxygenase, was employed to investigate oxidation differences between various lipids and corresponding flavor formation patterns. Lipase treatment resulted in higher levels of non‑oxygenated volatile compounds and saturated aldehydes, whereas lipoxygenase treatment increased oxygenated compounds, particularly (E)-2-hexenal, 1-penten-3-one, and 2-pentylfuran.

Carbon dot-based molecularly imprinted polymer fluorescent sensor for the detection of propranolol in plasma.

Propranolol, a medication used to treat cardiovascular diseases, can be harmful when overdosed and hazardous to ecosystems if released into the environment. Here, a new molecularly imprinted fluorescent sensor was developed from carbon dots through a sol-gel method. Carbon dots served as both the fluorescent signal and the carrier, with propranolol as the template molecule and 3-aminopropyltriethoxysilane as the functional monomer to be grafted on carbon dots' surface.

The pathogenesis and targeted therapies of intervertebral disc degeneration induced by cartilage endplate inflammation.

Intervertebral disc degeneration (IVDD) is the leading cause of low back pain, where degeneration and death of nucleus pulposus cells within the intervertebral disc (IVD) can be obviously revealed. This degeneration can result in an imbalance in the extracellular matrix due to the loss of proteoglycans and water content, which can further lead to catabolic and anabolic dysfunction of the IVD. Recently, the dysfunction of cartilage endplate (CEP) during aging has drawn large attention due to its essential functions in contributing nutrient exchange and maintaining IVD homeostasis.

Tea marinating-induced improvement of quality in roasted chicken: The potential relationship between tea, flavor, and hazardous substances.

The levels of flavor compounds and hazardous compounds are important indicators for evaluating high-temperature roasted food. In this paper, the effect of tea pre-marination on non-volatile compounds, volatile compounds, and hazardous compounds in roasted chicken. The results showed that the total content of key umami non-volatile compounds in roasted chicken marinated with green tea, white tea, and black tea increased by 17.

Discovery, Biomanufacture, and Derivatization of Licorice Triterpenoids.

Triterpenoids are the major active constituents of licorice, a well-known traditional medicinal herb. Licorice triterpenoids, represented by glycyrrhizin and glycyrrhetic acid, have a high structural diversity and are excellent lead compounds for the development of potent pharmaceuticals. However, their further application can be limited by insufficient activities, low bioavailability, and the presence of side effects, as well as the inefficiency of traditional plant extraction processes for compound production.

Primary intestinal NK/T-cell lymphoma complicated by intestinal perforation.

We report the case of a 32-year-old female patient who presented with worsening symptoms of bloody diarrhea, abdominal pain, and fever. Based on the clinical presentation, colonoscopy findings, and CT imaging, the patient underwent colectomy for suspected intestinal perforation. Histopathological examination and immunohistochemistry analysis of the resected specimen ultimately led to the diagnosis of primary intestinal NK/T-cell lymphoma.

Temporal variation of soil phosphorus fractions and nutrient stoichiometry during wetland restoration: Implications for phosphorus management.

Wetland restoration can promote the recovery of ecosystem services. However, an increasing number of reports indicate that phosphorus leaching risk occurs in downstream water bodies during the early stages of agricultural utilized wetland restoration, it is unclear that whether this phenomenon depend on temporal dynamics. Therefore, in this study, we used soil phosphorus fractions and stoichiometry as indicators to investigate soil phosphorus leaching and examine their evolution during both short- and long-term wetland restoration, aiming to identify the key driving factors.

The Effects of Non-Pharmacological Therapies for Dysphagia in Parkinson's Disease: A Systematic Review.

Background: Parkinson's disease (PD) is currently the second most common degenerative neurological disorder globally, with aspiration pneumonia caused by difficulty swallowing being the deadliest complication. The patient's subjective experience and the safety of swallowing have been the main focus of previous evaluations and treatment plans. The effectiveness of treatment may be attributed to the brain's ability to adapt and compensate.

Redox-sensitive epigenetic activation of SUV39H1 contributes to liver ischemia-reperfusion injury.

Liver ischemia-reperfusion (I/R) injury is a clinically relevant pathophysiological process that determines the effectiveness of life-saving liver transplantation, to which aberrant ROS accumulation plays a key role. In the present study we investigated the role of SUV39H1, a lysine methyltransferases, in this process focusing on regulatory mechanism and translational potential. We report that SUV39H1 expression was up-regulated in the liver tissues of mice subjected to ischemia-reperfusion and in hepatocytes exposed to hypoxia-reoxygenation (H/R) in a redox-sensitive manner.

Identification of novel drug targets for liver cirrhosis and its potential side-effects by human plasma proteome.

Liver cirrhosis, a common liver disease, currently lacks specific targeted therapies. This study investigates the potential therapeutic effects of serum circulating proteins on cirrhosis from a genetic perspective, and identified six associated plasma proteins (SERPINA1, PSG5, NCAN, APOE, ADH1B, GM2A). To search for therapeutic drugs associated with circulating proteins, databases such as DrugBank and DGIdb are utilized.

KRT80 in hepatocellular carcinoma plays oncogenic role via epithelial-mesenchymal transition and PI3K/AKT pathway.

Hepatocellular carcinoma (HCC), a globally prevalent form of cancer, is featured by aggressive growth and early metastasis. Elucidating the underlying mechanism and identifying the effective therapy are critical for advanced HCC patients. In the study, we detect that KRT80 was upregulated in HCC samples.

Comparative multi-omics analyses uncover the distinct quality characteristics of different fish soup varieties.

Fish soup has become an integral part of global cuisine, appreciated for its rich nutritional benefits and delectable flavor. This study comparatively evaluated the quality characteristics of fish soups derived from three freshwater fish species and three seawater fish species using multi-omics analysis. The primary aroma characteristics of fish soup were meaty and greasy, closely associated with the oxidation of lipids, particularly unsaturated triglycerides.

NSUN5 Facilitates Hepatocellular Carcinoma Progression by Increasing SMAD3 Expression.

Hepatocellular carcinoma (HCC) is characterized by frequent intrahepatic and distant metastases, resulting in a poor prognosis for patients. Epithelial-mesenchymal transition (EMT) plays a pivotal role in this process. However, the expression of NOP2/Sun RNA methyltransferase 5 (NSUN5) in HCC and its role in mediating EMT remain poorly understood.

A Hollowed-Out Heterometallic Cluster for Catalytic Knoevenagel Condensation.

Lanthanide-containing clusters are synthetically challenging and with significant chemical and materials applications. Herein, two isostructural heterometallic clusters of the formula (NO)@[LnNi(OH)(IDA)(CHCOO)(NO)(HO)]Cl⋅xHO⋅yCHOH (IDA=iminodiacetate; Ln=Gd 1, x=110, y=0; Ln=Eu 2, x=95, y=40) were obtained via co-hydrolysis of Ln (Gd or Eu) and Ni in the presence of iminodiacetate (IDA). Crystallographic studies show that each features a truncated tetrahedral core of LnNi within which a void of 1.

An improved invagination pancreaticojejunostomy technique associated with a lower incidence of pancreatic fistula: A single-center study.

Purpose: This study compared the incidence of postoperative pancreatic fistula (POPF) between standard invagination pancreaticojejunostomy (PJ) and an improved PJ technique after pancreaticoduodenectomy and evaluated the clinical utility of the improved PJ procedure.

Methods: Clinical and postoperative data of 363 patients who underwent pancreaticoduodenectomy at the First Affiliated Hospital of Soochow University from February 2018 to October 2021 were analyzed retrospectively. In our cohort, 155 patients underwent the improved PJ technique (group A), and 208 underwent standard invagination PJ (group B).

Tim-1-mediated extracellular matrix promotes the development of hepatocellular carcinoma.

Tim-1 (T-cell immunoglobulin and mucin domain 1), also known as Kim-1 (kidney injury molecule 1) or hepatitis A virus cellular receptor 1 (HAVCR1), is a transmembrane protein expressed on various immune and epithelial cells. It plays a role in modulating inflammatory and immune responses. In this study, we find that Tim-1 is overexpressed in hepatocellular carcinoma (HCC) samples and that its expression is significantly correlated with postoperative survival.

The role of short-chain fatty acid metabolism in the pathogenesis, diagnosis and treatment of cancer.

Short-chain fatty acids (SCFAs), which are saturated fatty acids consisting of six or fewer carbon atoms, have been found to be closely associated with the biological behavior of malignant tumors. This manuscript provides a comprehensive review on the role of SCFAs in regulating cell cycle, apoptosis, tumor angiogenesis, epithelial-mesenchymal transition, protein regulatory pathways, and histone regulation in promoting the development of malignant tumors. Furthermore, we discuss the potential therapeutic strategies targeting SCFAs for treating malignant tumors.

Application of the IMB Model in the Vision of Zero Harm Caused by Magnetic Resonance Ferromagnetic Projection Accidents.

Objective: The aim of the study is to explore the application of safety education based on the IMB model to prevent harm caused by magnetic resonance ferromagnetic projection accidents.

Methods: One hundred ninety-six patients undergoing magnetic resonance imaging were divided into a control group of 90 cases and an observation group of 106 cases. The control group received routine safety education, while the observation group received safety education based on the IMB model in addition to routine education.

Evaluation of the performance of nursing students taught using online and offline learning models in emergency and critical care nursing during the COVID-19 pandemic.

Aim: The study investigated the outcomes of employing this hybrid method among nursing students engaged in emergency and critical care curricula during the COVID-19 pandemic.

Methods: A retrospective study examined nursing students enrolled in the nursing program in China between January 2019 and January 2021. Students were divided into two groups: a study group that engaged in hybrid learning and a control group that participated in traditional classroom learning.