Self-assembled recombinant camel serum albumin nanoparticles-encapsulated hemin with peroxidase-like activity for colorimetric detection of hydrogen peroxide and glucose.

The construction of enzyme mimics using protein protection layers possesses advantages of high biocompatibility and superior catalytic activity, which is desirable for biomedical applications including diseases diagnosis. Here, from E. coli expression system, recombinant protein of camel serum albumin (rCSA) from Camelus bactrianus was successfully obtained to encapsulate hemin via the self-assemble method without additional toxic organic reagents.

A green electrolysis of silver-decorated MoS nanocomposite with an enhanced antibacterial effect and low cytotoxicity.

To tackle the devastating microbial infections for the public health, a continuous search for effective and safe nanobiocides based on their prominent nanoscale effects has been extensively explored during past decades. In this study, a green electrolysis method was employed to synthesize silver-doped molybdenum sulfide (Ag@MoS) composite materials. The obtained nanocomposites exhibited a sheet-like structure with a large specific surface area, which contributed to the efficient loading and refined distribution of silver nanoparticles.

Nanobodies derived from Camelids represent versatile biomolecules for biomedical applications.

Nanobodies are antigen binding variable domains of heavy-chain antibodies without light-chains, and these biomolecules occur naturally in the serum of Camelidae species. Nanobodies have a compact structure and low molecular weight when compared with antibodies, and are the smallest active antigen-binding fragments. Because of their remarkable stability and manipulable characteristics, nanobodies have been incorporated into biomaterials and used as molecular recognition and tracing agents, drug delivery systems, molecular imaging tools and disease therapeutics.

Bactrian camel serum albumins-based nanocomposite as versatile biocargo for drug delivery, biocatalysis and detection of hydrogen peroxide.

In response to extreme environmental conditions, Bactrian camels with largest population in China have evolved with the unique and extraordinary stress-tolerant mechanism in the bodies, in which the most abundantly secreted serum albumins contribute to an important role in diverse physiological activities such as maintaining osmotic pressure and transporting endogenous/exogenous molecules. In this study, we have for the first time purified Chinese Bactrian camel serum albumins (CSA) aimed at exploring their biomedical application. The mass spectrometric as well as structural analysis of CSA have revealed the sequence consensus and alpha-helix abundant structures among its heterologous proteins.

GSH-responsive curcumin/doxorubicin encapsulated Bactrian camel serum albumin nanocomposites with synergistic effect against lung cancer cells.

The aim of this study was to prepare camel serum albumin (CSA) nanoparticles using a self-assembly strategy to co-immobilize curcumin (CCM) and doxorubicin (Dox) which was in favor of combined chemotherapy and biomedical applications of bactrian ( ) CSA. The constructed CSA nanoparticles (CSA-NPs) with the size around 200 nm displayed a high degree of polydispersity and further encapsulation of CCM and Dox caused no apparent morphological changes to the nanocomposite (CCM/Dox CSA-NPs). The synergistic cytotoxic effect of CCM and Dox on cancer cell A549 was observed with the calculated combination index less than 1.

Synthesis and experiments of carcinoma vascular endothelial targeting polymeric nano-micelles combining small particle size and supermagnetic sensitivity.

To construct carcinoma vascular endothelial-targeted polymeric nanomicelles with high magnetic resonance imaging (MRI) sensitivity and to evaluate their biological safety and in vitro tumor-targeting effect, and to monitor their feasibility using clinical MRI scanner. Amphiphilic block copolymer, poly(ethylene glycol)--poly(ε-caprolactone) (PEG-PCL) was synthesized via the ring-opening polymerization of ε-caprolactone (CL) initiated by poly(ethylene glycol) (PEG), in which cyclic pentapeptide Arg-Gly-Asp (cRGD) was conjugated with the terminal of hydrophilic PEG block. During the self-assembly of PEG-PCL micelles, superparamagnetic γ-FeO nanoparticles (11 nm) was loaded into the hydrophobic core.

The prevalence of lacunar infarct decreases with aging in the elderly: a case-controlled analysis.

Background And Purpose: Lacunar infarct (LI) is well known as a heterogeneous primary disorder of cerebral small vessel. Compelling results have demonstrated that age is a risk factor to the prevalence of LI. However, the relationship between age and the prevalence of LI remains obscure.

[A preliminary study of dual-source computed tomography dual energy imaging in the diagnosis of acute traumatic bone marrow lesions in spine].

Objective: To explore the feasibility of virtual noncalcium technique from dual-source computed tomography (DSCT) for the diagnosis of vertebral bone marrow lesions.

Methods: A total of 13 patients with acute vertebral bone marrow lesions underwent both DSCT and MRI within 3 days. And the DSCT dual-energy CT data were postprocessed for generating virtual noncalcium images and color-coded maps.

Clinical, pathological, and imaging characteristics of primitive neuroectodermal tumors of the spine.

Primitive neuroectodermal tumors (PNETs) located in the spine are extremely rare, and information concerning these tumors in the medical literature is limited to single case reports. This pictorial essay presents the clinical, pathological, and imaging characteristics of PNET of the spine.