Selenium-sensitive histone deacetylase 2 is required for forkhead box O3A and regulates extracellular matrix metabolism in cartilage.

Introduction: Selenium (Se) as well as selenoproteins are vital for osteochondral system development. Se deficiency (SeD) has a definite impact on the expression and activity of histone deacetylases (HDACs). Abnormal expression of some HDACs affects cartilage development.

Evaluation and perception of online teaching of molecular biology using DingTalk for international medical students during the COVID-19 pandemic.

The COVID-19 pandemic has greatly impacted the education of international students. The authors taught a molecular biology course using the DingTalk platform for international medical students (IMS) in the autumn semester of 2020. We assessed the effect of this online teaching based on an online questionnaire and by analysis of the final examination scores.

Identification of a cartilage specific novel miRNA which directly targets PRMT3 in rats.

Unlabelled: Through experiments to testify a candidate novel miRNA previously discovered by us is a real miRNA and involved in cartilage development.

Design: The and the newly hairpin miRNA transcribed sequence () was verified as a genuinely existing miRNA by northern blotting. The predicted secondary structure, sequence alignment and targets of were performed by "RNAstructure 5.

The impact and evaluation of COVID-19 pandemic on the teaching model of medical molecular biology course for undergraduates major in pharmacy.

Molecular biology is a very important basic course for undergraduates major in pharmacy. During the novel coronavirus epidemic, we first adopted an online teaching of molecular biology course with rain class and tencent meeting for undergraduates major in pharmacy, following a blended teaching mode. Finally, we evaluated the effect of this special-time teaching by analyzing the anonymous questionnaire and final examination scores.

Intervening upregulated SLC7A5 could mitigate inflammatory mediator by mTOR-P70S6K signal in rheumatoid arthritis synoviocytes.

Objective: The disruption of metabolic events and changes to nutrient and oxygen availability due to sustained inflammation in RA increases the demand of bioenergetic and biosynthetic processes within the damaged tissue. The current study aimed to understand the molecular mechanisms of SLC7A5 (amino acid transporter) in synoviocytes of RA patients.

Methods: Synovial tissues were obtained from OA and RA patients.

Upregulated PKM2 in Macrophages Exacerbates Experimental Arthritis via STAT1 Signaling.

Recent studies indicate that glucose metabolism is altered in rheumatoid arthritis. We hypothesize that Pkm2, as a key regulatory enzyme of glycolysis pathway, triggers the activation of macrophages (Mφ), which results in proinflammatory cytokine production during the arthritis progress. In this study, Pkm2 was found to be overexpressed in ED1-positive Mφ in spleens and synovial tissues from arthritic rats via immunofluorescence, Western blotting, and quantitative RT-PCR.

The mechanisms of lncRNA GAS5 in cardiovascular cells and its potential as novel therapeutic target.

Long noncoding RNAs (lncRNAs) are a large class of non (protein)-coding RNAs, which are longer beyond 200 nucleotides. LncRNA GAS5 is widely considered as a tumour suppressor in cell proliferation, apoptosis, cell migration and invasion of tumour cells. Recently, a growing body of evidences indicated that GAS5 was also widely involved in the pathologic process of cardiovascular cells, including regulation of apoptosis and inflammatory injury of cardiomyocytes; proliferation, apoptosis, autophagy and angiogenesis of endothelial cells; and proliferation, migration, apoptosis and differentiation of VSMCs.

The mechanism of lncRNA H19 in fibrosis and its potential as novel therapeutic target.

Fibrosis is widely observed in multiple organs, which is generally associated with aging-related diseases, including liver fibrosis, lung fibrosis, cardiac fibrosis and renal fibrosis. The excess deposition of extracellular matrix ultimately leads to the disruption of organ architecture and loss of function. H19 is paternally imprinted maternally expressed lncRNA, which is highly expressed during the development of embryo but quickly downregulated after birth.

The Human Novel Gene LNC-HC Inhibits Hepatocellular Carcinoma Cell Proliferation by Sequestering hsa-miR-183-5p.

Hepatocellular carcinoma (HCC) is the most commonly diagnosed cancer and the leading cause of cancer mortality. Several lines of evidence have demonstrated the aberrant expression of long noncoding RNAs (lncRNAs) in carcinogenesis and their universal regulatory properties. A thorough understanding of lncRNA regulatory roles in HCC pathology would contribute to HCC prevention and treatment.

Long noncoding RNAs as novel players in the pathogenesis of hypertension.

Long noncoding RNAs (lncRNAs) are non-(protein)-coding RNAs longer than ~200 nucleotides and have been reported to be involved in multiple human diseases by regulating gene expression. A growing body of evidence has demonstrated that lncRNAs are also widely implicated in mechanisms of hypertension, including regulation of the proliferation, migration, and apoptosis of VSMCs; the production of iNOS and NO; and the angiogenic function of endothelial cells. Several lncRNAs were also differentially expressed in the renal and cardiac tissues of hypertensive rats and even in placental samples from preeclampsia patients.

Long Noncoding RNA lnc-HC Regulates PPARγ-Mediated Hepatic Lipid Metabolism through miR-130b-3p.

Nonalcoholic fatty liver disease (NAFLD) is due to the excessive lipid accumulation within hepatocytes. Metabolic nuclear receptors (MNRs) play great roles in lipid homeostasis. We have identified a novel long noncoding RNA (lncRNA), lnc-HC, which regulates hepatocytic cholesterol metabolism through reducing Cyp7a1 and Abca1 expression.

Aberrant expression of long noncoding RNAs in the serum and myocardium of spontaneous hypertensive rats.

Circulating long noncoding RNAs as biomarkers of diseases have attracted increasing attention recently. However, circulating lncRNAs in hypertension is still unexplored niche. The levels of lncRNAs GAS5, NR024118, MRAK134679, AX765700 and MRNR026574 were measured in the serum and myocardium of hypertensive rats and normal controls with real time PCR.

Abnormal Expression of Leads to Dysregulation of Inflammatory Effectors in Human Synoviocytes.

Dysregulation of multiple microRNAs widely takes place during rheumatoid arthritis (RA) and experimental arthritides. This study is performed to explore the possible mechanism underlying deficiency-mediated inflammation in human synoviocytes SW982. Firstly, RNAi of led to increased COX2, MMP3, and MMP13 protein production, while overexpression could reduce MMP13 expression.

Circular RNAs as novel regulators, biomarkers and potential therapies in fibrosis.

Fibrosis is the excess deposition of extracellular matrix components which ultimately leads to the disruption of organ architecture and loss of function. Circular RNAs (circRNAs) are a newly discovered type of long noncoding RNAs with single-stranded covalently closed loops. It is known that circRNAs are novel regulators of gene expression via various ways, including miRNA sponge, protein sponge, regulation of transcription and post transcription.

Selenium-sensitive miRNA-181a-5p targeting SBP2 regulates selenoproteins expression in cartilage.

Selenium (Se) deficiency brings about defects in the biosynthesis of several selenoproteins and has been associated with aberrant chondrogenesis. Selenocysteine (Sec) Insertion Sequence (SECIS) and SECIS binding protein 2 (SBP2) interaction is a very critical node for the metabolic balance between Se and selenoproteins. The Gpx1, Gpx4 and SelS have different binding affinities with SBP2 in cells.

Pristane induces autophagy in macrophages, promoting a STAT1-IRF1-TLR3 pathway and arthritis.

Autophagy is involved in both innate and adaptive immune regulation. We propose that autophagy regulates activation of TLR3 in macrophages and is thereby essential for development of pristane-induced arthritis. We found that pristane treatment induced autophagy in macrophages in vitro and in vivo, in spleen cells from pristane injected rats.

Circulating long noncoding RNAs as novel biomarkers of human diseases.

Long noncoding RNAs (lncRNAs) are a kind of noncoding RNAs which are longer than ˜200 nucleotides, lacking of protein-encoding capacity and are implicated in the pathogenesis of various diseases. Recently, it was demonstrated that lncRNAs could be released into the circulation and be stable in blood. Circulating lncRNAs have been reported to have potential in distinguishing patients from healthy individuals.

Comparison of the efficacy of tenofovir monotherapy versus tenofovir-based combination therapy in adefovir-experienced chronic hepatitis B patients: a systematic review and meta-analysis.

Background: Chronic hepatitis B virus (HBV) infection remains a major public health problem worldwide. Tenofovir monotherapy or tenofovir-based combination therapy have achieved promising results in the treatment of chronic hepatitis B patients who failed adefovir therapy.

Objective: The goal of this study was to assess the efficacy of tenofovir monotherapy compared with tenofovir-based combination therapy for treatment of adefovir-experienced chronic hepatitis B (CHB) patients.

Efficacy of tenofovir-based rescue therapy in patients with lamivudine-resistant hepatitis B virus: A systematic review and meta-analysis.

Background: Currently, there are no conclusive results on the efficacy of Tenofovir disoproxil fumarate (TDF) monotherapy in chronic hepatitis B (CHB) patients with lamivudine-resistant (LAM-R).

Objective: The aim of this study was to compare the efficacy between TDF and TDF-based combination therapy against LAM-R HBV in CHB patients.

Methods: Randomized and non-randomized control trials directly comparing TDF and TDF-based therapy for treatment of LAM-R CHB patients, were searched in Pubmed, Medline, EMBASE, database up to June 15, 2015.

Abnormality of epiphyseal plate induced by selenium deficiency diet in two generation DA rats.

This study aimed to observe the effects of Se deficiency on epiphyseal plates of two generation DA rats fed with artificial total synthetic low Se diet. All F0 and F1 DA rats were fed with synthetic low Se diet (SeD group) and low Se diet supplied with Se (SeS group). The levels of selenium and enzyme activities of GPx were detected in plasma of the rats.

The emerging roles of long noncoding RNAs in common cardiovascular diseases.

Long noncoding RNAs (lncRNAs) are defined as noncoding RNAs that are longer than ~200 nucleotides and lack protein-encoding capacity. It has been shown that lncRNAs are involved in multiple human diseases by regulating gene expression at various levels. However, studies of lncRNAs in the cardiovascular system are still in their infancy.

Expression profiling of long noncoding RNAs and the dynamic changes of lncRNA-NR024118 and Cdkn1c in angiotensin II-treated cardiac fibroblasts.

A growing body of evidence shows that long non-coding RNAs (lncRNAs) are involved in multiple human diseases than previously realized. However, no information is available now about lncRNAs in cardiac fibroblasts. The expression profile of lncRNAs was analyzed in Ang II-treated cardiac fibroblasts using lncRNAs arrays.

Angiotensin II upregulated the expression of microRNA-224 but not microRNA-21 in adult rat cardiac fibroblasts.

The role of microRNA-21 (miRNA-21, miR-21) in cardiac fibrosis remains controversial, while the role of microRNA-224 (miRNA-224, miR-224) in cardiac fibroblasts has not been reported. Angiotensin II (Ang II) is known to play a pivotal role in the pathogenesis of cardiac fibrosis. The aim of this study was to confirm whether the expression of miR-21 and miR-224 is regulated by Ang II in adult rat cardiac fibroblasts.