Chromosome-level genome assembly of tetraploid Chinese cherry (Prunus pseudocerasus).

Chinese cherry belongs to the family Rosaceae, genus Prunus, and has high nutritional and economic value. 'Duiying' is a Chinese cherry variety local to Beijing, and has better performance than sweet cherry in terms of disease resistance. However, disease resistance resources of 'Duiying' have not been fully exploited partially due to the lack of a high-quality genome.

Dual RNA-Seq Reveals Temperature-Mediated Gene Reprogramming and Molecular Crosstalk between Grapevine and .

High temperatures associated with a fluctuating climate profoundly accelerate the occurrence of a myriad of plant diseases around the world. A comprehensive insight into how plants respond to pathogenic microorganisms under high-temperature stress is required for plant disease management, whereas the underlying mechanisms behind temperature-mediated plant immunity and pathogen pathogenicity are still unclear. Here, we evaluated the effect of high temperature on the development of grapevine canker disease and quantified the contribution of temperature variation to the gene transcription reprogramming of grapevine and its pathogenic agent using a dual RNA-seq approach.

The woody plant-degrading pathogen Lasiodiplodia theobromae effector LtCre1 targets the grapevine sugar-signaling protein VvRHIP1 to suppress host immunity.

Lasiodiplodia theobromae is a causal agent of Botryosphaeria dieback, which seriously threatens grapevine production worldwide. Plant pathogens secrete diverse effectors to suppress host immune responses and promote the progression of infection, but the mechanisms underlying the manipulation of host immunity by L. theobromae effectors are poorly understood.

An NmrA-Like Protein, Lws1, Is Important for Pathogenesis in the Woody Plant Pathogen .

The NmrA-like proteins have been reported to be important nitrogen metabolism regulators and virulence factors in herbaceous plant pathogens. However, their role in the woody plant pathogen is less clear. In the current study, we identified a putative NmrA-like protein, Lws1, in and investigated its pathogenic role via gene silencing and overexpression experiments.

A Putative Effector LtCSEP1 from Inhibits BAX-Triggered Cell Death and Suppresses Immunity Responses in .

is a causal agent of grapevine trunk disease, and it poses a significant threat to the grape industry worldwide. Fungal effectors play an essential role in the interaction between plants and pathogens. However, few studies have been conducted to understand the functions of individual effectors in .

Systemic Identification and Functional Characterization of Common in Fungal Extracellular Membrane Proteins in .

Plant pathogenic fungi deploy secreted proteins into apoplastic space or intracellular lumen to promote successful infections during plant-pathogen interactions. In the present study, fourteen CFEM domain-containing proteins were systemically identified in and eight of them were functionally characterized. All eight proteins were confirmed to be secreted into extracellular space by a yeast signal peptide trapping system.

LtEPG1, a Secretory Endopolygalacturonase Protein, Regulates the Virulence of in and Is Recognized as a Microbe-Associated Molecular Patterns.

The genome encodes numerous glycoside hydrolases involved in organic matter degradation and conducive to pathogen infection, whereas their molecular mechanisms are still largely unknown. Here, we identified the glycoside hydrolase family 28 endopolygalacturonase LtEPG1 in and characterized its function in detail. LtEPG1 acts as a virulence factor during infection.

Genome-Wide Identification of Long Non-coding RNAs Responsive to Infection in Grapevine.

Long non-coding RNAs (lncRNAs) refer to a class of RNA molecules that are longer than 200 nucleotides and do not encode proteins. Numerous lncRNAs have recently emerged as important regulators of many biological processes in animals and plants, including responses to environmental stress and pathogens. dieback is one of the more severe grapevine trunk diseases worldwide.

Transcriptional response of grapevine to infection with the fungal pathogen Lasiodiplodia theobromae.

Botryosphaeria dieback on the grapevine is caused by Botryosphaeriaceae fungi, which threatens the yield and quality of grapes. At present, chemical control strategies are often observed to be ineffective in controlling the disease worldwide. Improving our understanding of the molecular mechanisms that confer resistance to pathogens would facilitate the development of more pathogen-tolerant grape varieties.

Dlf1, a WRKY transcription factor, is involved in the control of flowering time and plant height in rice.

Flowering time and plant height are important agronomic traits for crop production. In this study, we characterized a semi-dwarf and late flowering (dlf1) mutation of rice that has pleiotropic effects on these traits. The dlf1 mutation was caused by a T-DNA insertion and the cloned Dlf1 gene was found to encode a WRKY transcription factor (OsWRKY11).