Ulp1 Regulates Cell Proliferation Through INO1 in .

Background/objectives: Ulp1 is a vital regulator of the cell cycle, with its absence leading to G2/M phase arrest in . This study aims to investigate the role of Ulp1 in cell cycle regulation in and to elucidate its mechanisms of action, particularly through the modulation of the gene .

Methods: We generated Ulp1 knockout strains in using the FLP-FRT system and performed RNA sequencing (RNA-seq) to analyze gene expression changes.

Purification and Initial Functions of Sex-Specific Storage Protein 2 in Bombyx mori.

In this study, we identified a heat-resistant protein from the chrysalis stage of the silkworm which we named sex-specific storage protein 2 (SSP2). This protein was stable even at 80 °C, and has an amino acid sequence that is 90.65 % homologous to SP2.

Purification and functional characterization of a protein: Bombyx mori human growth hormone like protein in silkworm pupa.

Human growth hormone (hGH) is a peptide hormone secreted by eosinophils of the human anterior pituitary, and a regulatory factor for a variety of metabolic pathways. A 30-kD protein from the pupa stage of silkworm was detected by Western blotting and confirmed by immunoprecipitation based on its ability to bind to anti-hGH antibody. This protein, named BmhGH-like protein, was purified from fresh silkworm pupas through low-temperature homogenization, filtration, and centrifugation to remove large impurity particles.

All-trans retinoic acid affects subcellular localization of a novel BmNIF3l protein: functional deduce and tissue distribution of NIF3l gene from silkworm (Bombyx mori).

A novel cDNA sequence encoding a predicted protein of 271 amino acids containing a conserved NIF3 domain was found from a pupal cDNA library of silkworm. The corresponding gene was named BmNIF3l (Bombyx mori NGG1p interacting factor 3-like). It was found by bioinformatics that BmNIF3l gene consisted of five exons and four introns and BmNIF3l had a high degree of homology to other NIF3-like proteins, especially in the N-terminal and C-terminal regions.

Molecular characterization and immunohistochemical localization of a novel troponin C during silkworm development.

We have cloned and sequenced a novel Bombyx mori gene that encodes a protein having a high degree of homology with other known troponin C (TnC) proteins. The amino acid sequence, DX[DN]X[DSG]X(6)E, a highly conserved putative Ca(2+) -binding motif found in loops within the globular domains of previously identified TnC proteins, is also present in BmTnC. We have expressed and purified to homogeneity a His-tagged BmTnC fusion protein having a molecular weight of approximately 21.