Melatonin inhibits osteosarcoma stem cells by suppressing SOX9-mediated signaling.

Aims: Melatonin (N-acetyl-5-methoxytryptamine) has been reported to suppress epithelial-mesenchymal transition and cancer stem cells in some types of cancer. However, the effects of melatonin on the osteosarcoma stem cells, epithelial-mesenchymal transition and metastasis of osteosarcoma are still not clear. The present study was conducted to dissect the activity of melatonin on the osteosarcoma stem cells and the underlying mechanisms.

Downregulation of microRNA 99a in oral squamous cell carcinomas contributes to the growth and survival of oral cancer cells.

MicroRNAs (miRNAs) are small non-coding RNA molecules that regulate gene expression by either translational inhibition or mRNA degradation. miRNAs play pivotal roles in physiological functioning and pathological progression. The function of microRNA-99a (miR-99a) in oral squamous cell carcinoma (OSCC) remains unclear.

Piperine suppresses tumor growth and metastasis in vitro and in vivo in a 4T1 murine breast cancer model.

Aim: To investigate the effects of piperine, a major pungent alkaloid present in Piper nigrum and Piper longum, on the tumor growth and metastasis of mouse 4T1 mammary carcinoma in vitro and in vivo, and elucidate the underlying mechanisms.

Methods: Growth of 4T1 cells was assessed using MTT assay. Apoptosis and cell cycle of 4T1 cells were evaluated with flow cytometry, and the related proteins were examined using Western blotting.

IFN-γ producing T cells contribute to the increase of myeloid derived suppressor cells in tumor-bearing mice after cyclophosphamide treatment.

It has been reported that treatment with cyclophosphamide (CTX) as a chemotherapeutic drug in cancer patients or tumor-bearing mice can result in an increase in the proportion of myeloid derived suppressor cells (MDSCs) in blood and lymphoid organs. Here we sought to clarify the possible mechanism of this unwanted increase in proportion of MDSCs in tumor-bearing mice after CTX treatment. We found that both CD4(+) T cells and CD8(+) T cells underwent an expansion and activation before the increase of MDSCs in the early period of CTX treatment in 4T1 breast tumor-bearing mice.

[Clinical study of carbon dioxide laser tonsillectomy].

Objective: To investigate the therapeutic effect of carbon dioxide laser tonsillectomy.

Method: In this prospective, randomized study, One hundred and two patients were divided into laser group or control group. Patients of laser group were cured with carbon dioxide laser tonsillectomy, and the control group was cured with routine method.

[Oral-facial-digits syndrome I: case report].

To report a case of oral-facial-digits syndrome I who underwent plastic surgery of tongue, palate and digital abnormalities. The tongue of the patient restored normal mobility approximately but the speech function could not recover completely. The function recovery of the patient depended on the degree of mental retardation, its possible mechanism was the mutation of OFD I gene.

[The use of self-reinforced P(L/D)LA bioabsorbable internal fixation system in oral maxillofacial fractures].

Purpose: The purpose of this study was to evaluate the effectiveness of self-reinforced P(L/D)LA internal fixation system for oral maxillofacial fractures.

Methods: The system was used in 43 patients. The reduction situation during and after operation, postoperative complications and degradation time were recorded.

[Antiangiogenesis effect of linomide in treatment of transplanted human oral carcinoma in nude mice and its relations to regulation on cytokine secretion of macrophage].

OBJECTIVE: To investigate the antiangiogenesis effect of linomide in treatment of transplanted human squamous cell carcinoma of tongue in BALB/C nude mice and to study its relations to regulation on cytokine secretion of macrophages. METHODS: An animal model was established by inoculating the human squamous cell carcinoma cell line Tca8113 into the BALB/c nu/nu nude mice. The mice were randomly divided into three groups and received linomide therapy.