Using amino acid waste liquid as functional supplement to change microbial community in up-flow anaerobic sludge blanket treatment of methanolic wastewater.

In this study, amino acid waste liquid was employed as a functional supplement (designated as amino acid-rich FS) in the up-flow anaerobic sludge blanket (UASB) treatment of methanolic wastewater. The effect of amino acid-rich FS was evaluated through repeated batch tests, showing that a 0.5% and 1% dosage increased the maximum methane production rate by 93.

Microbial electron flow promotes naphthalene degradation in anaerobic digestion in the presence of nitrate electron acceptor: Focus on electron flow regulation and microbial interaction succession.

Microbial electron flow (MEF) is produced from microbial degradation of organic compounds. Regulating MEF to promote organic pollutants biodegradation such as naphthalene (Nap) is a potential way but remains a lack of theoretical basis. Here, we regulated MEF by adding electron acceptor NO to achieve 2.

Response of methanogenic system to long-term polycyclic aromatic hydrocarbon exposure: Adsorption and biodegradation, performance variation, and microbial function assessment.

Phenanthrene (PHE) as a typical polycyclic aromatic hydrocarbon (PAH) is prevalent and harmful to organisms in petroleum-polluted sites. The effects of PHE concentration levels on performance, microbial community and functions in methanogenic system were comprehensively investigated by an operation of UASB reactor (198 days) and a series of batch tests. The results found that PHE was prone to accumulate in reactor by sludge adsorption (Final concentration = 12.

Production of struvite by magnesium anode constant voltage electrolytic crystallisation from anaerobically digested chicken manure slurry.

Nitrogen and phosphorus levels in livestock manure and digestive fluid are high, posing a threat to soil and water quality and necessitating nutrient removal and recovery. Phosphorus recovery has the potential to alleviate the global phosphorus resource crisis. This study proposed a magnesium anode constant voltage electrolysis method to crystallise struvite (magnesium ammonium phosphate hexahydrate, MgNHPO·6HO) from anaerobically digested chicken manure slurry using reaction kinetics at variable constant voltages ranging from 2 V to 12 V.

Biochar assisted cellulose anaerobic digestion under the inhibition of dodecyl dimethyl benzyl ammonium chloride: Dose-response kinetic assays, performance variation, potential promotion mechanisms.

This study evaluated the inhibitory effect and mitigation strategy of dodecyl dimethyl benzyl ammonium chloride (DDBAC) suppression on anaerobic digestion. With the 12 h-suppression, only 16.64% of anaerobes were alive, and acetotrophic methanogens were significantly inhibited.

Response of earthworm coelomocytes and catalase to pentanone and hexanone: a revelation of the toxicity of conventional solvents at the cellular and molecular level.

Organic solvents like 2-pentanone and 2-hexanone which are widely used in industrial production make up a large proportion of the source of chemical pollution. What is worrisome is that the cellular and molecular toxicity of 2-pentanone and 2-hexanone has not been reported yet. Based on this, earthworms and catalase (CAT) were chosen as target receptors for the toxicity studies.

Efficient pollutants removal and microbial flexibility under high-salt gradient of an oilfield wastewater treatment system.

The treatment of hypersaline oilfield wastewater (HSOW) is a challenge due to its complex composition and low biodegradability, especially in coastal areas. In this study, an HSOW treatment system of gas flotation and biochemistry technology combined with constructed wetland (CW) was investigated. The combined treatment system could efficiently remove COD, NH-N and oil under high salinity (1.

[Bmim]FeCl mediated inhibition and toxicity during anaerobic digestion: Dose-response kinetics, biochar-dependent detoxification and microbial resistance.

[Bmim]FeCl, or 1‑butyl‑3-methylimidazolium tetrachloroferrate, is a typical ionic liquid (IL). Its recyclable, magnetic, multicomponent, and solvent-free nature makes it a particularly attractive ionic liquid for use in industrial processes. Despite its widespread use, the potential hazards that [Bmim]FeCl might pose to the environment, including productive microorganisms, have not been explored.

Single and simultaneous effects of naphthalene and salinity on anaerobic digestion: Response surface methodology, microbial community analysis and potential functions prediction.

Polycyclic aromatic hydrocarbons (PAHs) are a persistent and prevalent class of pollutants in petroleum-contaminated saline environment, which pose potential harm to organisms. Researches on anaerobic biodegradation of PAHs are gradually emerging, but the response of anaerobic microorganisms to salinity changes and the co-effects of salinity and PAHs in anaerobic digestion (AD) system have seldom been reported. Thus, we investigated the variations of AD system performance and anaerobic microbial community caused by different concentrations of naphthalene (Nap) or/and NaCl based on Box-Behnken Design (0 mg/L ≤ Nap ≤150 mg/L, 0 g/L ≤ NaCl ≤25 g/L).

The dosage-effect of biochar on anaerobic digestion under the suppression of oily sludge: Performance variation, microbial community succession and potential detoxification mechanisms.

This study investigated the dosage-effect of biochar on the suppressed mesophilic digestion of oily sludge (OS) containing naphthalene (recalcitrant compound) and starch (easily bioavailable substrate). Methanogenesis was inhibited in control with OS, where biomethane yield (63.33 mL/gVS) was obviously lower than theoretical yield (260.

Toxic effect and mechanism of ultrafine carbon black on mouse primary splenocytes and two digestive enzymes.

This paper investigated the toxic effect and mechanism of ultrafine carbon black (UFCB) on splenocytes and enzymes in the digestive system. It was found that the toxicity of UFCB to splenocytes was dose-dependent. UFCB with a low concentration (<15 μg/mL) had no significant effect on splenocytes while UFCB with high concentration (>15 μg/mL) induced significant oxidative damage with increased content of reactive oxygen species (ROS) (134%) and malonaldehyde (MDA) (222.

Anaerobic co-digestion of chicken manure and cardboard waste: Focusing on methane production, microbial community analysis and energy evaluation.

This study aimed to investigate the synergistic effect and microbial community changes between chicken manure (CM) and cardboard (CB) during anaerobic co-digestion. Meanwhile, the energy balance of biogas engineering was extrapolated based on the batch tests. In batch tests, co-digestion system achieved the highest improvement (14.

Investigation of the process stability of different anammox configurations and assessment of the simulation validity of various anammox-based kinetic models.

Over the last 30 years, the successful implementation of the anammox process has attracted research interest from all over the world. Various reactor configurations were investigated for the anammox process. However, the construction of the anammox process is a delicate topic in regards to the high sensitivity of the biological reaction.

Study of the effects of ultrafine carbon black on the structure and function of trypsin.

Due to the rapid development of industrial society, air pollution is becoming a serious problem which has being a huge threat to human health. Ultrafine particles (UFPs), one of the major air pollutants, are often the culprits of human diseases. At present, most of the toxicological studies of UFPs focus on their biological effects on lung cells and tissues, but there are less researches taking aim at the negative effects on functional proteins within the body.

Catalase and superoxide dismutase response and the underlying molecular mechanism for naphthalene.

Naphthalene, a naturally-occurring polyaromatic hydrocarbon, pose potential threats to health for its wide exposures in environment. Naphthalene could disrupt the redox equilibrium resulting in oxidative damage. Antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD) are considered to be the efficient defense barriers to protect organisms from negative impacts of toxicants.

Cytotoxicity of perfluorodecanoic acid on mouse primary nephrocytes through oxidative stress: Combined analysis at cellular and molecular levels.

Long-chain perfluoroalkyl acids (PFAAs) such as perfluorodecanoic acid (PFDA) are toxic, persistent organic pollutants. This study investigated the harmful effect of PFDA on mouse primary nephrocytes and its mechanism at cellular and molecular levels. Cellular results showed that PFDA exhibited nephrotoxicity with decreased cell viability and increased apoptosis.

Enhancement methane fermentation of Enteromorpha prolifera waste by Saccharomyces cerevisiae: batch kinetic investigation, dissolved organic matter characterization, and synergistic mechanism.

With the invasion of green tide, there were millions of tons of Enteromorpha prolifera (Enteromorpha) that need to be disposed of. An efficient microecological system for Enteromorpha fermentation was constructed using Saccharomyces cerevisiae (S. cerevisiae) and granular sludge at mesophilic condition (35 °C).

Biodegradable organic matter-containing ammonium wastewater treatment through simultaneous partial nitritation, anammox, denitrification and COD oxidization process.

For both nitrogen and COD removal from biodegradable organic matter (BOM)-containing ammonium wastewater, the simultaneous partial nitritation, anammox, denitrification and COD oxidization (SNADCO) process is a promising solution. In this study, with the stable influent ammonium concentration of 250.0 mg/L (nitrogen loading rate of 0.

The toxic effects of alizarin red S on catalase at the molecular level.

Alizarin red S (ARS) is a widespread mordant dye derived from alizarin. However, it was reported to be mutagenic and carcinogenic probably because it could induce oxidative damages in organisms. Catalase (CAT) is an important antioxidant enzyme defensing oxidative damages induced by xenobiotics.

Perfluorodecanoic acid-induced oxidative stress and DNA damage investigated at the cellular and molecular levels.

Perfluorodecanoic acid (PFDA) has been widely used in production of many daily necessities because of its special nature. Althoughtoxic effects of PFDA to organisms have been reported, there is little research on the genotoxicity induced by oxidative stress of PFDA on the cellular and molecular levels simultaneously. Thus, we investigated the DNA oxidative damage caused by PFDA in mouse hepatocytes.

Exploring the effects of operational mode and microbial interactions on bacterial community assembly in a one-stage partial-nitritation anammox reactor using integrated multi-omics.

Background: The metabolic capacities of anammox bacteria and associated microbial community interactions in partial-nitritation anammox (PNA) reactors have received considerable attention for their crucial roles in energy-efficient nitrogen removal from wastewater. However, a comprehensive understanding of how abiotic and biotic factors shape bacterial community assembly in PNA reactors is not well reported.

Results: Here, we used integrated multi-omics (i.

Accelerated bio-methane production rate in thermophilic digestion of cardboard with appropriate biochar: Dose-response kinetic assays, hybrid synergistic mechanism, and microbial networks analysis.

The effect of biochar on the thermophilic digestion of mono-cardboard was investigated. Compared with control group (T0), the maximum rate of biomethane production was significantly improved after the addition of biochar, especially, it has been improved by 40.6% in T1 (0.

Enhanced anaerobic performance and SMD process in treatment of sulfate and organic S-rich TMBA manufacturing wastewater by micro-electric field-zero valent iron-UASB.

This study focused on investigating reactor performance, simultaneous methanogeneis and denitrifiction (SMD) process for treatment of a sulfate plus organic sulfur - rich 3,4,5-Triethoxybenzaldehyde (TMBA) manufacturing wastewater with variable COD/TSO (total sulfate) ratio by micro-electric field- zero-valent-iron (ZVI) UASB for 390 days. The initial COD/TSO was set as 1.42, 0.

PFOA and PFOS interact with superoxide dismutase and induce cytotoxicity in mouse primary hepatocytes: A combined cellular and molecular methods.

This study investigated the adverse effects of perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS) on mouse primary hepatocytes by conducting cell viability, apoptosis, intracellular oxidative stress level, superoxide dismutase (SOD), catalase (CAT) activity and glutathione level assays. It was shown that PFOA and PFOS altered antioxidant enzymes activities and triggered oxidative stress, and thus exhibited cytotoxicity to the hepatocytes. Molecular mechanisms of SOD activities were measured and structural changes were explored by isothermal titration calorimetry and multiple spectroscopy.

Revealing the correlation of biomethane generation, DOM fluorescence, and microbial community in the mesophilic co-digestion of chicken manure and sheep manure at different mixture ratio.

Batch co-digestion tests of chicken manure (CM) and sheep manure (SM) at different ratio (R) were conducted under mesophilic condition (35 °C). Batch kinetic analysis of bioCH production, excitation-emission matrix (EEM) fluorescence of dissolved organic matter (DOM), and microbial community were investigated. The well-fitted modified Gompertz model (R, 0.