A375 melanoma-derived lactate controls A375 melanoma phenotypes by inducing macrophage M2 polarization TCA cycle and TGF-β signaling.

Introduction: Macrophage phenotypes have been linked to progression and prognosis of cutaneous melanoma. However, the association between Warburg effect in A375 melanoma and macrophages polarization, as well as the underlying mechanisms, remains less well documented.

Objective: The present study aimed to investigate the effect of lactate derived from A375 melanoma on macrophage polarization, melanoma phenotype responses and the underlying mechanisms.

Applications of interpretable ensemble learning for workplace risk assessment: The Chinese coal industry as an example.

Machine learning has demonstrated potential in addressing complex nonlinear changes in risk assessment. However, further exploration is needed to enhance model interpretability and optimize performance. Therefore, this study aims to develop a novel workplace risk assessment framework.

UDA-seq: universal droplet microfluidics-based combinatorial indexing for massive-scale multimodal single-cell sequencing.

The use of single-cell combinatorial indexing sequencing via droplet microfluidics presents an attractive approach for balancing cost, scalability, robustness and accessibility. However, existing methods often require tailored protocols for individual modalities, limiting their automation potential and clinical applicability. To address this, we introduce UDA-seq, a universal workflow that integrates a post-indexing step to enhance throughput and systematically adapt existing droplet-based single-cell multimodal methods.

Erratum: The potential value of cuprotosis (copper-induced cell death) in the therapy of clear cell renal cell carcinoma.

[This corrects the article on p. 3947 in vol. 12, PMID: 36119838.

Hybrid-Electrolytes System Established by Dual Super-lyophobic Membrane Enabling High-Voltage Aqueous Lithium Metal Batteries.

Aqueous electrolytes and related aqueous rechargeable batteries own unique advantage on safety and environmental friendliness, but coupling high energy density Li-metal batteries with aqueous electrolyte still represent challenging and not yet reported. Here, this work makes a breakthrough in "high-voltage aqueous Li-metal batteries" (HVALMBs) by adopting a brilliant hybrid-electrolytes strategy. Concentrated ternary-salts ether-based electrolyte (CTE) acts as the anolyte to ensure the stability and reversibility of Li-metal plating/stripping.

Frontiers in sarcopenia: Advancements in diagnostics, molecular mechanisms, and therapeutic strategies.

The onset of sarcopenia is intimately linked with aging, posing significant implications not only for individual patient quality of life but also for the broader societal healthcare framework. Early and accurate identification of sarcopenia and a comprehensive understanding of its mechanistic underpinnings and therapeutic targets paramount to addressing this condition effectively. This review endeavors to present a cohesive overview of recent advancements in sarcopenia research and diagnosis.

An enhanced multi-attribute crowd evacuation model incorporating the effects of environmental impact factors.

We propose an enhanced floor field model (FFM) to analyze the behavioral characteristics of crowds with varying attributes proportions during evacuation. This model governs pedestrian movement through the Dynamic Floor Field (DFF) and the Static Floor Field (SFF). The DFF takes into account individual factors such as the gender, familiarity with the environment, and social relationships of evacuees, which influence safe evacuation.

Correlational analysis of occupational accidents and the safety policies in the Chinese coal mining industry from 2008 to 2021.

This study investigates the correlation between previous coal mine safety policies and accidents in China. Data on coal mine accidents and government regulatory information from 2008 to 2021 are collected. The characteristics of coal mine accidents are analyzed, and safety policy indexes are identified.

[Retracted] Isoquercitrin inhibits bladder cancer progression and by regulating the PI3K/Akt and PKC signaling pathways.

Following the publication of this paper, it was drawn to the Editor's attention by a concerned reader that the agarose gel electrophoretic bands shown in Fig. 4A for PKC were strikingly similar to bands that had already appeared in another article written by different authors at different research institutes. Owing to the fact that the contentious data in the above article had already been published prior to its submission to , the Editor has decided that this paper should be retracted from the Journal.

Fate of the second task in dual-task interference is associated with sensory system interactions with default-mode network.

Psychological refractory period (PRP) effect refers to the delay in responding to the second of two tasks occurring in rapid succession. While all the major models of PRP highlight the importance of the frontoparietal control network (FPCN) in prioritizing the neural processing of the first task, the fate of the second task remains poorly understood. Here, we provide novel neural evidence on how the functional connectivity between sensory systems and the default-mode network (DMN) suspends the neural processing of the second task to ensure the efficient completion of the first task in dual-task situation.

The mechanism and clinical application of DNA damage repair inhibitors combined with immune checkpoint inhibitors in the treatment of urologic cancer.

Urologic cancers such as kidney, bladder, prostate, and uroepithelial cancers have recently become a considerable global health burden, and the response to immunotherapy is limited due to immune escape and immune resistance. Therefore, it is crucial to find appropriate and effective combination therapies to improve the sensitivity of patients to immunotherapy. DNA damage repair inhibitors can enhance the immunogenicity of tumor cells by increasing tumor mutational burden and neoantigen expression, activating immune-related signaling pathways, regulating PD-L1 expression, and reversing the immunosuppressive tumor microenvironment to activate the immune system and enhance the efficacy of immunotherapy.

Review and prospect of immune checkpoint blockade therapy represented by PD-1/PD-L1 in the treatment of clear cell renal cell carcinoma.

As a common tumor of the urinary system, the morbidity and mortality related to renal carcinoma, are increasing annually. Clear cell renal cell carcinoma (CCRCC) is the most common subtype of renal cell carcinoma, accounting for approximately 75% of the total number of patients with renal cell carcinoma. Currently, the clinical treatment of ccRCC involves targeted therapy, immunotherapy, and a combination of the two.

Numerical Analysis of Fracture Behaviour for Cracked Joints in Corrugated Plate Girders Repaired by Stop-Holes.

The efficient crack eliminated stop-hole measure was proposed to repair and reduce the stress concentration associated fracture risk of the corrugated plate girders by setting it at the critical joint of flange plate with tightened bolts and gaskets under preloading. To investigate the fracture behaviour of these repaired girders, parametric finite element analysis was conducted, focusing on the mechanical feature and stress intensity factor of crack stop-hole in this paper. The numerical model was verified against experimental results first, and then the stress characteristics due to the presence of crack open-hole were analysed.

Coaxial 3D Printing of Zeolite-Based Core-Shell Monolithic Cu-SSZ-13@SiO Catalysts for Diesel Exhaust Treatment.

Core-shell catalysts with functional shells can increase the activity and stability of the catalysts in selective catalytic reduction of NO with ammonia. However, the conventional approaches based on multistep fabrication for core-shell structures encounter persistent restrictions regarding strict synthesis conditions and limited design flexibility. Herein, a facile coaxial 3D printing strategy is for the first time developed to construct zeolite-based core-shell monolithic catalysts with interconnected honeycomb structures, in which the hydrophilic noncompact silica serves as shell and Cu-SSZ-13 zeolite acts as core.

Chemical Synthesis of Innovative Silver Nanohybrids with Synergistically Improved Antimicrobial Properties.

Background: The wide use of antibiotics has created challenges related to antibiotic-resistant bacteria, which have been increasingly found in recent decades. Antibiotic resistance has led to limited choices of antibiotics. Multiple old antimicrobial agents have high antimicrobial properties toward bacteria, but they unfortunately also possess high toxicity toward humans.

FIPRESCI: droplet microfluidics based combinatorial indexing for massive-scale 5'-end single-cell RNA sequencing.

Single-cell RNA sequencing methods focusing on the 5'-end of transcripts can reveal promoter and enhancer activity and efficiently profile immune receptor repertoire. However, ultra-high-throughput 5'-end single-cell RNA sequencing methods have not been described. We introduce FIPRESCI, 5'-end single-cell combinatorial indexing RNA-Seq, enabling massive sample multiplexing and increasing the throughput of the droplet microfluidics system by over tenfold.

Research on the Characteristics of Safety Culture and Obstacle Factors among Residents under the Influence of COVID-19 in China.

This study established a comprehensive evaluation indicator model for the safety culture among residents during COVID-19 and an obstacle degree model for the identification of the major factors affecting the residents' safety culture. The results show that the overall level of the safety culture among residents was 0.6059.

Targeting the Glycolytic Enzyme PGK1 to Inhibit the Warburg Effect: A New Strategy for Keloid Therapy.

Background: Aerobic glycolysis (the Warburg effect) may play an important role in keloid pathogenesis, which may be aggravated by the hypoxic microenvironment in keloids. Phosphoglycerate kinase 1 (PGK1), a key glycolytic enzyme, is essential for cellular aerobic glycolysis, but its role in keloid formation remains unknown. This study aimed to detect PGK1 expression in keloid tissue and investigate the effects of inhibiting PGK1 expression on keloid fibroblasts (KFbs) under hypoxia and normoxia.

Anti-Inflammatory Effects of the 35kDa Hyaluronic Acid Fragment (B-HA/HA35).

Background: Hyaluronic acid (HA) and HA fragments interact with a variety of human body receptors and are involved in the regulation of various physiological functions and leukocyte trafficking in the body. Accordingly, the development of an injectable HA fragment with good tissue permeability, the identification of its indications, and molecular mechanisms are of great significance for its clinical application. The previous studies showed that the clinical effects of injectable 35kDa B-HA result from B-HA binding to multiple receptors in different cells, tissues, and organs.

PI3K/AKT pathway promotes keloid fibroblasts proliferation by enhancing glycolysis under hypoxia.

Our previous study demonstrated altered glucose metabolism and enhanced phosphorylation of the PI3K/AKT pathway in keloid fibroblasts (KFb) under hypoxic conditions. However, whether the PI3K/AKT pathway influences KFb cell function by regulating glucose metabolism under hypoxic conditions remains unclear. Here, we show that when PI3K/AKT pathway was inactivated with LY294002, the protein expression of glycolytic enzymes decreased, while the amount of mitochondria and mitochondrial membrane potential increased.