Isolation of salt-tolerant Vibrio alginolyticus X511 for efficient co-production of 2,3-butanediol and alginate lyase from Laminaria japonica.

In order to establish an efficient microbial transformation platform based on seaweed feedstocks, experiments were performed to isolate a salt-tolerant strain capable of producing alginate lyase and 2,3-butanediol (2,3-BDO). Its physiological and biochemical characteristics, carbon source utilization, and product synthesis capabilities were investigated, and then the process for co-producing alginate lyase and 2,3-BDO from Laminaria japonica was optimized. Results showed that the isolated strain was identified as Vibrio alginolyticus, which was capable of utilizing multiple carbon sources to produce alginate lyase and 2,3-BDO even in the presence of 5 % NaCl.

Green manure combined with reduced nitrogen reduce NH emissions, improves yield and nitrogen use efficiencies of rice.

Background: Green manure is an important source of organic fertilizer. Exploring green fertilizer and nitrogen fertilizer reduction is important for agricultural production. However, few studies have been conducted, especially on the effects of different green fertilizers along with reduced nitrogen fertilizer application on soil ammonia volatilization emissions, rice yield, and nitrogen fertilizer uptake and utilization.

Integrated metabolomics and network pharmacology revealed the key active ingredients for the treatment of ulcerative colitis in the Citrus reticulata 'Dahongpao' peel.

Citrus reticulata pericarpium (CRP), the peel of Citrus reticulata 'Dahongpao' (DHP) is a medicinal herb with significant therapeutic value for treating ulcerative colitis (UC). However, the active therapeutic components of CRP are unclear. This study aims to reveal the metabolites potentially associated with the pharmacological properties of CRP.

Spinning a Liquid Wheel and Driving Surface Thermomagnetic Convection with Light.

A typical Tesla thermomagnetic engine employs a solid magnetic wheel to convert thermal energy into mechanical energy, while thermomagnetic convection in ferrofluid is still challenging to observe because it is a volume convection that occurs in an enclosed space. Using a water-based ferrofluid, a liquid Tesla thermomagnetic engine is demonstrated and reports the observation of thermomagnetic convection on a free surface. Both types of fluid motions are driven by light and observed by simply placing ferrofluid on a cylindrical magnet.

sp. nov., sp. nov. and sp. nov. with the organophosphorus pesticides degrading ability isolated from mangrove sediments.

Members of the genus were frequently isolated from polluted environments and possess great bioremediation potential. Here, three species, designated B2637, B2580 and B1949, were isolated from mangrove sediments and might represent novel species in the genus based on a polyphasic taxonomy study. Phylogenomic analysis revealed that strains B2580, B1949 and B2637 clustered with NBRC 102051, '' F72 and 502str22, respectively.

sp. nov., an algicidal bacterium isolated from seawater.

A taxonomic study was carried out on strain BGMRC 0090, which was isolated from seawater. The isolate was a Gram-negative, aerobic, flagellated, rod-shaped bacterium with algicidal activity. Optimal growth was observed at 30 °C, pH 6.

sp. nov., an endophytic bacterium isolated from the root of .

A novel endophytic bacterium, designated strain BGMRC 0089, was isolated from a surface-sterilized root of . Cells were observed to be Gram-negative, rod-shaped and motile with polar flagella. Strain BGMRC 0089 was found to grow optimally at 28-30 °C, pH 7.

Development of a micro-Raman system for in vivo studying the mechanism of laser biological effects.

The laser irradiation on organism will produce a series of biological effects, which can be used for basic medical research, diagnosis and treatments of diseases. However, the mechanism of this biological effects is still unclear. As a sensitive molecular monitoring technique, Raman spectroscopy has became a very popular detection method in biomedical research especially in vivo study.

sp. nov. and sp. nov., two novel actinobacteria isolated from mangrove sediments.

Two novel actinobacteria with the ability to degrade kerosene, designated as B3033 and Y57, were isolated from mangrove sediments in Tieshan Harbour, South China Sea. Both strains are Gram-staining-positive, non-spore forming, slow-growing, oxidase-positive, non-motile and aerobic. Their major cellular fatty acids were C and Cω9.

sp. nov., with an antibacterial property, isolated from mangrove sediment.

A Gram-stain-negative, aerobic, milky white bacterium, designated B2012, was isolated from mangrove sediment collected at Beibu Gulf, South China Sea. Antimicrobial activity assay revealed that the isolate possesses the capability of producing antibacterial compounds. Strain B2012 shared the highest 16S rRNA gene sequence relatedness (96.

Thermohalobaculum xanthum gen. nov., sp. nov., a moderately thermophilic bacterium isolated from mangrove sediment.

A novel moderately thermophilic and halophilic bacterium, designated strain M0105, was isolated from mangrove sediment collected in the Beibu Gulf, south China. The isolate is Gram-negative, non-motile and rod-shaped bacterium with smooth colonies of pale-yellow appearance. Growth occurs at 15-46 °C (optimum 37-40 °C) and pH range of 6.

gen. nov., sp. nov., a novel bacterium isolated from a sea snail .

Members within the family are morphologically and genetically highly diverse, and originate mostly from coastal marine environments. In this study, a novel species of this family, designated M0103, was isolated from the surface of a sea snail . Strain M0103 is Gram-stain-negative, halophilic, non-motile and non-Bacteriochlorophyll -producing bacterium.

A four-protein metabolon assembled by a small peptide protein creates the pentacyclic carbonate ring of aldgamycins.

Organic carbonates (OCs) are a class of compounds featured by a carbonyl flanked by two alkoxy/aryloxy groups. They exist in either linear or cyclic forms, of which the majority encountered in nature adopt a pentacyclic structure. However, the enzymatic basis for pentacyclic carbonate ring formation remains elusive.

sp. nov., a heavy metal resistant bacterium isolated from coral.

A Gram-stain-negative, aerobic, mesophilic, non-motile bacterium, designated M0104, was isolated from a gorgonian coral collected from Xieyang island, Guangxi Province, PR China. Colonies of the strain were non-motile cocci and pink. The strain grew at 15-34 °C (optimum, 28 °C), pH 4.

Characterization of a bifunctional alginate lyase as a new member of the polysaccharide lyase family 17 from a marine strain BP-2.

Objectives: Bifunctional alginate lyase can efficiently saccharify alginate biomass and prepare functional oligosaccharides of alginate.

Results: A new BP-2 strain that produces alginate lyase was screened and identified from rotted Sargassum. A new alginate lyase, Alg17B, belonging to the polysaccharide lyase family 17, was isolated and purified from BP-2 fermentation broth by freeze-drying, dialysis, and ion exchange chromatography.

DEAD-Box Helicase 5 Interacts With Transcription Factor 12 and Promotes the Progression of Osteosarcoma by Stimulating Cell Cycle Progression.

Osteosarcoma (OS) is a common malignant primary bone tumor. Its mechanism of development and progression is poorly understood. Currently, there is no effective therapeutic regimens available for the treatment of OS.

Biosynthesis of an anti-tuberculosis sesterterpenoid asperterpenoid A.

A putative three-gene cluster for asperterpenoid A was identified. Step-wise reconstitution of this gene cluster in Aspergillus oryzae reveals that astC encodes a sesterterpene cyclase to synthesize preasperterpenoid A, which is dually oxidized by a P450 enzyme AstB to give asperterpenoid A along with a minor product asperterpenoid B, and asperterpenoid A is further oxidized by another P450 eznyme AstA to afford a new sesterterpenoid asperterpenoid C. Unexpectedly, asperterpenoids A and B, but not the final product asperterpenoid C, exhibit potent inhibitory activity against Mycobacterium tuberculosis protein tyrosine phosphatase B with IC50 values of 3-6 μM.

AlgM4: A New Salt-Activated Alginate Lyase of the PL7 Family with Endolytic Activity.

Alginate lyases are a group of enzymes that catalyze the depolymerization of alginates into oligosaccharides or monosaccharides. These enzymes have been widely used for a variety of purposes, such as producing bioactive oligosaccharides, controlling the rheological properties of polysaccharides, and performing structural analyses of polysaccharides. The gene of the marine bacterium M0101 encodes an alginate lyase that belongs to the polysaccharide lyase family 7 (PL7).

DDX3 binding with CK1ε was closely related to motor neuron degeneration of ALS by affecting neurite outgrowth.

Amyotrophic lateral sclerosis (ALS) is a chronic neurodegenerative disease characterized by progressive degeneration of motor neurons. The pathogenesis of ALS remains largely unknown. RNA helicase DDX3 is a multifunctional protein involved in several steps of gene expression.

Involvement of c-Fos in cell proliferation, migration, and invasion in osteosarcoma cells accompanied by altered expression of Wnt2 and Fzd9.

Osteosarcoma (OS) is an aggressive bone tumor, and proto-oncogene c-Fos is involved in this lethal disease. However, the role and molecular mechanism of c-Fos in the development and progression of OS remain enigmatic. As one of the Wnt family members, Wnt2 is closely associated with the development of several malignant tumors.

Characterization of Methyltransferase AlmCII in Chalcomycin Biosynthesis: The First TylF Family O-Methyltransferase Works on a 4'-Deoxysugar.

Sugar O-methylation is a ubiquitous modification in natural products and plays diverse roles. This realization has inspired many attempts to search for novel methyltransferases. Chalcomycins are a group of 16-membered macrolides containing two methylated sugars that require three methyltransferases for their biosynthesis.

The altered autophagy mediated by TFEB in animal and cell models of amyotrophic lateral sclerosis.

Autophagy is an intracellular degradation process that clears away aggregated proteins or aged and damaged organelles. Abnormalities in autophagy result in defects in clearance of these misfolded and aggregate proteins, which have been associated with neurodegenerative disorders. A key neuropathological hallmark of amyotrophic lateral sclerosis (ALS) that contributes to the progressive loss of motor neurons is abnormal protein aggregation of mutant Cu/Zn superoxide dismutase1 (SOD1).