A "pseudo" fluorescence indicator displacement assay based on "light-up" sanguinarine for identifying small ligands targeting HIV-1 RRE RNA.

Developing effective methods to identify drugs that can target HIV-1 Rev response element (RRE) RNA and block the interaction between Rev and RRE has practical significance in the treatment of AIDS. Fluorescence indicator displacement (FID) assay was commonly employed to identify small ligands binding to RNA. In this study, the non-fluorescent sanguinarine (Sang) was used as a novel "pseudo" fluorescence indicator to identify small ligands targeting RRE through its fluorescence "light-up", assisted by β-cyclodextrin (β-CD) or nano-SiO.

Ligand "switching on" fluorescence of HIV-1 RNA-templated copper nanoclusters for ligand-RNA interaction assays.

Ligand-RNA interaction assay provides the basis for developing new RNA-binding small molecules. In this study, fluorescent copper nanoclusters (CuNCs) were first prepared using two kinds of HIV-1 RNA targets, rev-responsive element (RRE) and transactivator response element (TAR) RNA, as new templates, and it was found that the fluorescence of the single RNA-templated CuNCs was negligible. Using neomycin as a model drug, the fluorescence could be augmented (approximately 6 times) for the neomycin/RNA-templated CuNCs.

Fed-batch fermentation of Tuber melanosporum for the hyperproduction of mycelia and bioactive Tuber polysaccharides.

For the first time, a fed-batch fermentation process of Tuber melanosporum was developed for the efficient production of bioactive mycelia and Tuber polysaccharides. Each 1.67 g/L of peptone and 8.