Electrochemical tuning of the activity and structure of a copper-cobalt micro-nano film on a gold electrode, and its application to the determination of glucose and of Chemical Oxygen Demand.

Micro-nano structured Cu-Co was in situ fabricated on the surface of a gold electrode via electrochemical reduction of CuCl and Co(NO). It is shown that the shape of the particles can be controlled by variation of deposition current, deposition time, pH value and the ratio of Cu(II) and Co(II) ions. If prepared under current of -200 μA in 0.

Rapid and selective determination of urinary lysozyme based on magnetic molecularly imprinted polymers extraction followed by chemiluminescence detection.

A rapid, low cost and selective chemiluminescence method coupled with magnetic molecularly imprinted polymers extraction was developed to detect lysozyme in human urine samples. Compared with traditional solid-phase extraction, this method could achieve selective extraction for the lysozyme, avoid the time consuming elution from a column or centrifugation steps, and then showed great potential in the high-throughput screening of clinical samples. The parameters affecting the performance of extraction and chemiluminescence were investigated.

[Acclimating, screening of dominant bacteria for degrading pentachlorophenol and its degradation condition study].

Objective: To acclimate, screen the dominant bacteria for degrading pentachlorophenol and its growth characteristics and degradation conditions study.

Methods: Active sludge aeration and method of density gradient were used to acclimate the sludge. Using pentachlorophenol as a sole carbon source, it was increased step by step in continuous eight weeks.

[Preparation and evaluation of Sudan Red I molecularly imprinted polymer].

The molecularly imprinted polymer (MIP) was prepared by precipitation polymerization using Sudan Red I as the template. To investigate the influence of porogenic solvent and the amount of template on recognition property, selective chromatographic evaluation and frontal chromatography were performed. The results indicated that the obtained MIP had the best affinity and selectivity to the template when the molar ratio of template to functional monomer was 1:8 and a mixture of 30 mL methanol and 10 mL acetonitrile was used as the porogenic solvent.

delta-Aminolevulinic acid dehydratase activity, urinary delta-aminolevulinic acid concentration and zinc protoporphyrin level among people with low level of lead exposure.

To evaluate the relationship of delta-aminolevulinic acid dehydratase (ALAD) activity, urinary delta-aminolevulinic acid (ALAU) level and blood zinc protoporphyrin (ZPP) concentration to low blood lead (PbB) levels, these biomarkers were determined for all subjects enrolled from a rural area of southeast China where people had low levels of exposure to lead. The mean values of PbB, ALAD, ALAU and ZPP were 67.11 microg/L (SD: 1.

A stable and sensitive testing system for potential carcinogens based on DNA damage-induced gene expression in human HepG2 cell.

In order to analyze potential carcinogenic and genotoxic responses caused by exposure to pollutants existing in environment, a screening method has been established in our laboratory that uses a stably transfected HepG2 cell lines containing gadd153 promoter regions which drive a luciferase reporter gene. Activation of the exogenous gadd153 promoter was quantified using the luciferase activity following drug exposure. Twenty four agents were used to evaluate this screening assay.

A quantitative DNA methylation assay using mismatch hybridization and chemiluminescence.

Objective: To develop a quantitative method for methylation analysis of the p16 gene based on mismatch hybridization and chemiluminescence.

Methods: Genomic DNA was modified by sodium bisulfite to convert all unmethylated but not methylated cytosines to uracil, and subsequently a pair of primer having no CpG sites was designed for amplification target DNA containing methylated or unmethylated CpG sites. The PCR product spanning CpG sites were hybridized with two oligonucleotide probes which perfectly matched the methylated and unmethylated CpG sequences respectively, and the hybrids were detected by chemiluminescent method.

[Expression of DNA excision repair enzymes and lung cancer prognosis].

Objective: To study the expression levels of three DNA damage excision repair enzymes: excision repair cross-complementing rodent repair deficiency gene 2 (ERCC2), uracil DNA glycosylase (UDG), and proliferating cell nuclear antigen (PCNA), in different lung tissues and their relationship with lung cancer prognosis.

Methods: The expression levels of ERCC2, UDG, and PCNA protein were detected using immunohistochemistry method. Cancer tissues and normal tissues adjacent to the cancer from 61 cases of lung carcinoma, and tissue samples from 18 cases of benign lung disease were studied.

[Effect of benzo[a]pyrene on DNA damage and expression of genes involved in nucleotide excision repair in lung cancer cells].

Objective: To investigate the effect of benzo[a] pyrene(BaP) on DNA damage and expression of genes involved in nucleotide excision repair[xeroderma pigmentosum group B, C, G(XPB, XPC, XPG) and excision repair cross-complementing 1 (ERCC1)] in lung cancer A549 cells.

Methods: Cell survival was measured using MIT metabolic viability assay. Single cell gel assay was applied to determine the DNA damage and repair.

[Gene polymorphism of myeloperoxidase and genetic susceptibility to lung cancer].

Background & Objective: A-463 bp G/A polymorphism is located in the promoter region of myeloperoxidase (MPO) gene was found to be associated with lung cancer susceptibility; however, this association in Chinese population remained unclear. The aim of this study was designed to explore this association in Chinese population.

Methods: MPO genotypes in 98 cases of primary lung cancer and 112 persons of healthy control were detected using PCR-restriction fragment length polymorphism assay(PCR-RFLP) in a case-control molecular epidemiology study.

[Construction of nucleotide excision repair gene XPB antisense RNA expression plasmid and its functions].

Background & Objective: Nucleotide excision repair is an important pathway for cellular DNA damage repair. The drug resistance of tumor cell is often companied with the enhanced expression of DNA repair genes. Down-regulation of DNA repair capacity by antisense strategy can increase the drug sensitivity of tumor cells.

[Antisense ERCC1 RNA decreases the repair capability of damaged DNA in lung cancer cells induced by benzo[a]pyrene].

Objective: To investigate the effect of ERCC1 gene on the repair capability of damaged DNA in lung cancer A549 cells induced by benzo[a]pyrene.

Methods: Recombinant plasmid expressing ERCC1 antisense RNA was constructed and transfected into A549 cells by Lipofectin reagent. The stable-transfected cell colonies were selected by hygromycin.

[Study on the expression of DNA excision repair biomarkers in cispatin-treated lung cancer cell line].

Objective: To study the expression levels of ERCC2, UDG, and PCNA in cisplatin-treated A549 cell line.

Method: Comet assay, RT-PCR, and western blot were used to study the mRNA and protein expression levels of ERCC2, UDG, and PCNA.

Results: When treated with IC(20) cisplatin, the DNA damage level increased as the cisplatin treated time increased within 24 h of cisplatin treatment.