[Identification of oligopeptides mimicking the virus attachment protein of hantaan virus].

Objective: To identify and characterize the epitope associated with the virus attachment protein (VAP) of hantaan virus.

Methods: The monoclonal antibody 3G1 was used as the ligand to biospan from a phage-displayed 12-amino acid peptide library, then the positive phage clones were chosen and sequenced. The amino acid sequences of them were compared with that of hantaan virus G2 in homology.

[Purification and characterization of the fusion protein TGF-betaR II/Fc].

Aim: To express the fusion protein TGF-betaR II/Fc in large amounts by using recombinant Bac-TR II baculovirus expression system constructed by our laboratory and to purify and characterize it. Then, to verify whether the fusion protein TGF-betaR II/Fc can be able to block the biological activity of cytokine TGF-beta1.

Methods: The viral titer was determined by plaque forming test.

[Analysis on patial amino acid sequence of a novel glycoprotein binding to antiherpes simplex virus monoclonal antibody CHA9].

Aim: To acquire the characteristic amino acid sequence of a novel glycoprotein of herpes simplex virus (HSV), so as to localize gene encoding the novel glycoprotein accurately.

Methods: A 12-mer phage peptide library was screened for 3 rounds by using biotinylated mAb CHA9 against new glycoprotein g30k of HSV-2. Positive phage clones were detected by ELISA.

[Development and application of an indirect immunofluorescence assay for the detection of serum immunoglobulin G to human herpesvirus 6].

Aim: To establish an indirect immunofluorescence assay(IFA) for detection of serum IgG antibody against human herpesvirus 6(HHV-6).

Methods: Cord blood mononuclear cells infected by HHV-6 strain CN(5) were used to prepare cell antigen smear, so as to establish an IFA and make an epidemical investigation on serum specimens of child-bearing age, women.

Results: The specificity of the IFA for HHV-6 was confirmed by absorption assay(test).