Publications by authors named "Qiao-Yu Fang"
Carnosol exhibited ameliorating effects on muscle atrophy of mice developed cancer cachexia in our previous research. Here, the ameliorating effects of carnosol on the C2C12 myotube atrophy result from simulated cancer cachexia injury, the conditioned medium of the C26 tumor cells or the LLC tumor cells, were observed. To clarify the mechanisms of carnosol, the possible direct target proteins of carnosol were searched using DARTS (drug affinity responsive target stability) assay and then confirmed using CETSA (cellular thermal shift assay).
View Article and Find Full Text PDFCancer-derived exosomes are involved in the development of cancer cachexia. Carnosol, which exhibited ameliorating effects on cancer cachexia of C26 tumour-bearing mice in our previous study, alleviated atrophy of C2C12 myotubes induced by exosomes of C26 tumour cells in the present study. MiR-183-5p was found to be rich in C26 cells and C26 exosomes, and miR-183-5p mimic could directly induce atrophy of C2C12 myotubes.
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- The study aimed to create RNAi recombinant adenoviral vectors to specifically target and reduce the expression of GSK-3beta, a gene that plays a critical role in cell proliferation, and to analyze the influence of the Wnt/beta-catenin signaling pathway on the growth of human thyroid cells.
- Researchers constructed an adenovirus plasmid with RNAi targeting GSK-3beta, transfected it into HEK293A cells, and then tested its ability to knock down this gene in normal human thyrocytes, assessing its impact on cell proliferation and beta-catenin levels.
- Results showed that the GSK-3beta gene was significantly silenced in infected thyro