World J Gastroenterol
January 2015
Aim: To determine the role of NOB1, a regulator of cell survival in yeast, in human colorectal cancer cells.
Methods: Lentivirus-mediated small interfering RNA (siRNA) was used to inhibit NOB1 expression in RKO human colorectal cancer cells in vitro and in vivo in a mouse xenograft model. The in vitro and in vivo knockdown efficacy was determined using both Western blot and quantitative reverse transcription polymerase chain reaction (qRT-PCR).