ZntR is a critical regulator for zinc homeostasis and involved in pathogenicity in .

Zinc (Zn) is essential for all bacteria, but excessive Zn levels are toxic. Bacteria maintain zinc homeostasis through regulators, such as Zur, AdcR, and ZntR. is a significant pathogen causing acute serositis in ducks and other birds.

Molecular network strategies combined with MCnebula2 identify potential active compounds from steamed Polygonatum cyrtonema Hua.

Polygonatum cyrtonema Hua (PCH) underwent a series of transforms in its composition and had potential biological activities after steamed processing. In this study, LC-MS/MS datasets of chemical components in raw and steamed PCH were collected, and chemical profiling of PCH showed significant differences after steaming. Global Natural Products Social Molecular Network (GNPS-MN) combined with MCnebula2 were further used to annotate compound structures and data visualization.

Comprehensive analysis of lncRNAs and mRNAs revealed potential participants in the process of avian reovirus infection.

Avian reovirus (ARV), a double-stranded RNA virus, frequently induces immunosuppression in poultry, leading to symptoms such as irregular bleeding and spleen necrosis in infected ducks. Since 2017, the morbidity and mortality rates associated with ARV infection in poultry have been on the rise, progressively emerging as a significant viral disease impacting the duck farming industry in China. In our study, we collected duck embryo fibroblasts 18 h post-infection with ARV and conducted transcriptome sequencing analysis.

The functions of herpesvirus shuttling proteins in the virus lifecycle.

During viral infection, the transport of various proteins between the nucleus and cytoplasm plays an important role in the viral lifecycle. Shuttling proteins are key factors in the transmission of nucleocytoplasmic information within cells and usually contain nuclear localization signals and nuclear export signals to mediate correct positioning for themselves and other proteins. The nucleocytoplasmic transport process is carried out through the nuclear pore complex on the nuclear envelope and is mediated by specific protein carriers.

A Model to Identify Grey-Zone Patients with Chronic Hepatitis B Requiring Antiviral Therapy: A Multicentre, Retrospective Study.

Background: Individuals who do not match any specific stage in the natural history of chronic hepatitis B are classified into the grey-zone, and appropriate management for these patients remains unclear. This study aimed to develop and validate a non-invasive model to identify grey-zone patients requiring antiviral therapy (AVT).

Methods: We retrospectively collected data on 200 grey-zone patients not requiring AVT (according to assessment by non-invasive parameters from 2010 to 2023 in six hospitals) and randomised them into development (n=140) and validation (n=60) cohorts.

Benzalkonium chloride disinfection increases the difficulty of controlling foodborne pathogens identified in aquatic product processing.

Benzalkonium chloride (BAC) has been widely used as disinfectant in food processing, whereas there is still largely unknown concerning its profound and/or reverse effects on the pathogens control. This study was aimed to assess the effects of BAC disinfection on the controlling difficulty of foodborne pathogens, by investigating the growth rate, micromorphology, biofilm characteristics, and antibiotic resistance. The results showed that two pathogenic strains, identified as Proteus vulgaris PV29 and Bacillus cereus BC30, were residually remained on the processing utensils after BAC disinfection.

Exploring the therapeutic potential of small extracellular vesicles derived from induced pluripotent stem cell in periodontal regeneration.

Objectives: To investigate the role of small extracellular vesicles derived from induced pluripotent stem cells (iPSC-sEVs) in periodontal tissue regeneration, elucidate their potential molecular mechanisms, and provide theoretical guidance for the clinical application of iPSC-sEVs as a cell-free therapeutic strategy for periodontal tissue regeneration.

Methods: We investigated the effects of iPSC-sEVs on the proliferation, migration, and osteogenic differentiation of periodontal ligament stem cells (PDLSCs) in vitro. The regenerative potential of iPSC-sEVs was evaluated in vivo, using a periodontal defect model.

Preparation of alkali-activated oil shale residue-slag geopolymer-based binder using calcium carbide slag and sodium carbonate as alkali activator.

Based on the reuse of industrial solid wastes such as calcium carbide slag, slag, and oil shale residue, this work utilizes oil shale residue and slag as precursor materials, calcium carbide slag and sodium carbonate as alkaline activator to prepare a geopolymer-based binder. Mix proportion experiments of the geopolymer-based binder with varying oil shale residue contents were designed. Chemical composition and microstructure of the alkaline-activated oil shale residue-slag geopolymer-based binder system were analyzed using X-ray Fluorescence Spectrometer (XRF), X-ray Powder Diffractometer (XRD), and Scanning Electron Microscope (SEM).

β-ecdysone/PLGA composite scaffolds promote skull defect healing in diabetic rat.

Introduction: Diabetes mellitus often leads to bone metabolism disorders, hindering bone regeneration and delaying the healing of bone defects. β-Ecdysone, a plant-derived hormone known for its wide range of physiological activities, possesses hypoglycemic effects and promotes osteogenic differentiation. This study developed a composite PLGA slow-release scaffold loaded with β-ecdysone to enhance its bioavailability through topical administration and to investigate its potential to heal diabetic bone defects.

Pharmacokinetics and pharmacodynamics of ciprofol after continuous infusion in elderly patients.

Background: Ciprofol, a novel intravenous anesthetic, which has primarily been used for the induction and maintenance of general anesthesia in adults, is characterized by rapid onset, short duration of action, and quick and smooth recovery. However, the pharmacokinetic characteristics of continuous infusions and the correlation between the plasma concentration and the bispectral index (BIS) in elderly patients are still unknown.

Method: In this randomized, controlled study, thirty elderly patients (62-78 years old) undergoing elective gastrointestinal tumor resection were treated with propofol (N = 15) or ciprofol (N = 15) as sedatives during anesthesia.

The S-Phase Arrest of Host Cells Caused by an Alpha-Herpesvirus Genome Replication Facilitates Viral Recruitment of RNA Polymerase II to Transcribe Viral Genes.

Herpesviruses rely on host RNA polymerae II (RNA Pol II) for their mRNA transcription, yet the mechanisms of which has been poorly defined, while certain herpesviruses can enhance viral gene transcription by altering the RNA Pol II location, modulating its phosphorylation, or directly interacting with RNA Pol II. However, the influence of herpesviruses on RNA Pol II transcription extends beyond these direct effects. Here, we present a novel mechanism by which the host cell cycle regulates viral gene transcription via RNA Pol II during infection by Anatid Herpesvirus 1 (AnHV-1), an avian alpha-herpesvirus.

Single Rare-Earth Ion Doped Tin-Oxo Nanocluster Photoresists for High-Resolution Extreme Ultraviolet Lithography.

Rare-earth (RE) metals are known as industrial vitamins and show significant regulatory effects in many fields. In this work, we first demonstrated that the vitamin effect of RE metals can also be applied to extreme ultraviolet (EUV) lithography. Using a SnRE oxo cluster as the universal platform, different individual RE metal ions were successfully doped to obtain a series of isomorphic heterometallic clusters (RE = Y, Sm, Eu, Ho, Er).

Isolation and characterization of a broad-spectrum bacteriophage against multi-drug resistant Escherichia coli from waterfowl field.

Escherichia coli (E. coli) is a significant pathogen responsible for intestinal infections and foodborne diseases. The rise of antibiotic resistance poses a significant challenge to global public health.

Autoinducer-2 enhances the defense of against oxidative stress and DNA damage by modulation of c-di-GMP signaling via a two-component system.

As a universal language across the bacterial kingdom, the quorum sensing signal autoinducer-2 (AI-2) can coordinate many bacterial group behaviors. However, unknown AI-2 receptors in bacteria may be more than what has been discovered so far, and there are still many unknown functions for this signal waiting to be explored. Here, we have identified a membrane-bound histidine kinase of the pathogenic bacterium , AsrK, as a receptor that specifically detects AI-2 under low boron conditions.

DPV pUL15 possesses a potential NLS, which is important for the location of the terminase complex and for viral proliferation and genome cleavage.

In herpesvirus, the terminase subunit pUL15 is involved in cleavage of the viral genome concatemers in the nucleus. Previous studies have shown that herpes simplex virus 1 (HSV-1) pUL15 can enter the nucleus without other viral proteins and help other terminase subunits enter the nucleus. However, this study revealed that duck plague virus (DPV) pUL15 cannot localize independently to the nucleus and can only be localized in the nucleus in the presence of pUL28 and pUL33.

The characterization of outer membrane vesicles (OMVs) and their role in mediating antibiotic-resistance gene transfer through natural transformation in Riemerella anatipestifer.

Riemerella anatipestifer (R. anatipestifer, RA) is the etiological agent of duck serositis, an acute multisystemic disease in ducks that is globally distributed and causes serious economic losses in the duck industry. Despite exhibiting multidrug resistance, the transmission mechanism of its antibiotic resistance genes (ARGs) remains incompletely identified.

DHAV-1 3C protein promotes viral proliferation by antagonizing type I interferon via upregulating the ANXA2 protein.

The picornavirus 3C protein plays a crucial role in viral infection. One of its functions is inhibiting the immune response by cleaving or degrading innate immune-related proteins to promote viral infection. Annexin A2 (ANXA2) is a multifunctional host protein that plays a key role in various cellular processes, it also participates in viral infection.

Comparative genomics and phylogenetic analysis of six Malvaceae species based on chloroplast genomes.

The Malvaceae family, comprising 9 subfamilies and 4,225 species, includes economically significant taxa, such as Ceiba pentandra, Gossypium ekmanianum, Gossypium stephensii, Kokia drynarioides, Talipariti hamabo, and Durio zibethinus. Chloroplast (cp) genome research is crucial for elucidating the evolutionary divergence and species identification within this family. In this study, we assembled and annotated cp genomes of six Malvaceae species, conducting comprehensive comparative genomic and phylogenomic analyses.

Decoding the enigma: unveiling the transmission characteristics of waterfowl-associated -positive in select regions of China.

() serves as a critical indicator microorganism for assessing the prevalence and dissemination of antibiotic resistance, notably harboring various antibiotic-resistant genes (ARGs). Among these, the emergence of the gene represents a significant threat to public health, especially since carbapenem antibiotics are vital for treating severe infections caused by Gram-negative bacteria. This study aimed to characterize the antibiotic resistance features of -positive strains isolated from waterfowl in several regions of China and elucidate the dissemination patterns of the gene.

tRNA-Ser-UGA efficiently promotes the rapid release of duck hepatitis A virus from infected enterocytes and its remote dissemination to hepatocytes.

Enterocytes are a necessary portal for fecal-oral transmission of viruses, including duck hepatitis A virus (DHAV), that act on the absorption of amino acids (AAs). We note that the rapid death of ducklings caused by DHAV is likely due to its rapid release from enterocytes. However, the underlying mechanism driving the release of DHAV remains poorly understood.

Timing of Iliofemoral Vein Stent Implantation after Mechanical Thrombectomy for Acute Iliofemoral Venous Thrombosis.

Background: Comparison of the treatment effects of immediate stent implantation versus staged stent implantation after AngioJet mechanical thrombectomy in patients with acute iliofemoral venous thrombosis.

Methods: A study included 80 patients with acute iliofemoral venous thrombosis formed between June 2021 and February 2023. They were divided into 2 groups: the direct implantation group (37 patients, 9 males) and the staged implantation group (43 patients, 10 males).

Enhanced multistress tolerance of with the sugar transporter-like protein Stl1 mutation in the presence of glycerol.

Unlabelled: During microbial industrial production, microorganisms often face diverse stressors, including organic solvents, high salinity, and high sugar levels. Enhancing microorganism tolerance to such stresses is crucial for producing high-value-added products. Previous studies on the mechanisms of 2-phenylethanol (2-PE) tolerance in revealed a potential connection between the sugar transporter-like protein (Stl1) mutation (F427L) and increased tolerance to high sugar and salt stress, suggesting a broader role in multistress tolerance.

Identification and subcellular localization of proteins that interact with Duck plague virus pUL14 in infected host cells.

Duck plague (DP), which is caused by duck plague virus (DPV), is an infectious disease that severely harms the waterfowl breeding industry. The UL14 protein (pUL14) is a tegument protein encoded by the UL14 gene, which is located in the unique long (UL) region of the DPV genome. DPV pUL14 plays a crucial role in viral replication, likely by interacting with host and viral proteins that have yet to be identified.

Anti-tembusu virus of capsid-targeted viral inactivation delivered by lentiviral vector in vivo.

Tembusu virus (TMUV) is a member of genus flavivirus, which mainly causes decrease in production in egg ducks and neurological symptom in meatducks, causing serious economic losses to the poultry industry. Recently, the commercialized TMUV vaccines are mainly the WF100 live vaccine and the attenuated live vaccine (FX2010-180P), so it is particularly important to find new methods to combat TMUV. The capsid-targeted viral inactivation (CTVI) strategy is based on a viral core protein and an exogenous factor that can destroy viral DNA or RNA.