Genome-wide exploration and characterization of the RALFs and analysis of its role in peanut (Arachis hypogaea L.).

Background: Rapid alkalinization factors (RALFs) are small peptides hormones that regulate plant growth and stress responses. Although RALFs have been identified in a broad range of land plant species, their roles in peanuts (Arachis hypogaea L.) remain largely unexplored.

Using network analysis to identify central symptoms of depression and anxiety in different profiles of infertility patients.

Background: Depression and anxiety were not only common but also with serious consequence in infertility patients. The current study endeavors to define distinct depression and anxiety profiles of infertility patients and identify central symptoms within different profiles to facilitate targeted interventions.

Method: The research employed K-means Clustering to delineate the depression and anxiety profiles, followed by a repetition of the analysis using Latent Class Analysis (LCA).

Nanoengineered, ultrasmall and catalytic potassium calcium hexacyanoferrate for neuroprotection and temporal lobe epilepsy treatment.

Hippocampal sclerosis, characterized by significant hippocampal neuronal loss, oxidative stress, glial cell proliferation, and inflammatory responses, constitutes a pivotal component in the pathogenesis of temporal lobe epilepsy (TLE). Traditional treatment strategies, mainly involving anti-epileptic drugs, face challenges including ineffectiveness, drug tolerance, and adverse reactions, complicating management of the condition. Herein, we design and engineer ultrasmall potassium calcium hexacyanoferrate (III) nanoparticles, designated as KCaHNPs, which feature a broad spectrum of enzymatic activities analogous to superoxide dismutase, catalase, peroxidase, and glutathione peroxidase.

Sequential prenatal diagnosis of fetal skeletal dysplasia: A cohort study.

Introduction: Genetic factors are considered to be the main factors leading to fetal skeletal dysplasia (SD), and chromosomal microarray analysis (CMA) has been used clinically for the detection of SD fetuses. At present, whole exome sequencing (WES) has been applied in SD fetuses, but there is still a lack of data accumulation. The aim of this study is to perform sequential prenatal diagnosis for fetuses with SD indicated by ultrasound and to explore the clinical value of CMA followed by WES.

Correlation analysis of serum vitamin C and pelvic organ prolapse in middle-aged and elderly women: based on NHANES database.

Objective: This study aimed to evaluate the association between serum vitamin C and pelvic organ prolapse (POP) in middle-aged and elderly women.

Method: The 2005-2006 National Health and Nutrition Examination Survey (NHANES) database was used for cross-sectional study. The quartile method was employed to stratify the concentration of vitamin C.

Krüppel-like factor 9 alleviates Alzheimer's disease via IDE-mediated Aβ degradation.

The deposition of β-amyloid (Aβ) in the brain is a crucial factor in the pathogenesis of Alzheimer's disease (AD). Insulin-degrading enzyme (IDE) plays a critical role in the balance between Aβ production and degradation. However, the regulatory mechanisms of IDE are not yet fully understood.

Dezocine versus esketamine as postoperative analgesia in women undergoing cesarean section.

The objective of this was to investigate the effect of esketamine as a postoperative analgesia in women undergoing caesarean section. A total of 134 patients were divided into two groups: 112 received esketamine and 22 received dezocine, based on their voluntary choice of analgesic drugs for patient-controlled intravenous analgesia (PCIA). The postoperative analgesic effects were compared between the two groups.

The efficacy and safety of half-dose glucocorticoids combined with rituximab versus high-dose glucocorticoids for initial treatment of minimal change disease: a single-center experience.

Background: Minimal change disease (MCD) is a podocytopathy more commonly seen in children, but it also accounts for 10%-25% of adult nephrotic syndrome. High-dose oral glucocorticoids were recommended for initial treatment of MCD. However, long-term use of systemic corticosteroids is associated with significant adverse events, such as steroid-induced diabetes and infections.

Biallelic variants in SREBF2 cause autosomal recessive spastic paraplegia.

Hereditary spastic paraplegias (HSPs) refer to a genetically and clinically heterogeneous group of neurodegenerative disorders characterized by the degeneration of motor neurons. To date, a significant number of patients still have not received a definite genetic diagnosis. Therefore, identifying unreported causative genes continues to be of great importance.

Heavy gravity combined with light gravity local anesthetic in subarachnoid anesthesia for cesarean section did not reduce the incidence of intraoperative hypotension in maternal women: a prospective cohort study.

Background: Subarachnoid anesthesia is the primary anesthetic method for elective cesarean section surgery, characterized by rapidly taking effect and reliable analgesia. However, subarachnoid anesthesia is prone to cause a high block level, resulting in a high incidence of maternal hypotension. How to reduce the incidence of maternal hypotension under subarachnoid anesthesia is a practical problem that needs to be solved urgently in clinical practice.

Coptisine inhibits esophageal carcinoma growth by modulating pyroptosis via inhibition of HGF/c-Met signaling.

Esophageal carcinoma is a highly prevalent malignancy worldwide. The present study aimed to investigate the mechanism by which the natural compound coptisine affects pyroptosis in esophageal squamous cell carcinoma (ESCC). The expression of c-Met in ESCC patients was assessed by immunohistochemical analysis of tissue microarrays.

Soil aggregation alterations under soil microplastic and biochar addition and aging process.

Soil microplastics (MPs) are a substantial threat to soil health, particularly by disrupting soil aggregation. Additionally, MPs undergo aging processes in the soil, which may significantly alter their long-term impacts on soil structure. To investigate these effects, we conducted an eight-month soil incubation experiment, examining the influence of MPs and their aging on soil aggregation.

Intensifying Interfacial Reverse Hydrogen Spillover for Boosted Electrocatalytic Nitrate Reduction to Ammonia.

Rational regulation of active hydrogen (*H) behavior is crucial for advancing electrocatalytic nitrate reduction reaction (NORR) to ammonia (NH), yet in-depth understanding of the *H generation, transfer, and utilization remains ambiguous, and explorations for *H dynamic optimization are urgently needed. Herein we engineer a NiN nanosheet array intimately decorated with Cu nanoclusters (NF/NiN-Cu) for remarkably boosted NORR. From comprehensive experimental and theoretical investigations, the NiN moieties favors water dissociation to generate *H, and then *H can rapidly transfer to the Cu via unique reverse hydrogen spillover mediating interfacial Ni-N-Cu bridge bond, thus increasing *H coverage on the Cu site for subsequent deoxygenation/hydrogenation.

Fluorinated Porcine Bone-Derived Hydroxyapatite Promotes Vascularized Osteogenesis by Coordinating Human Bone Marrow Mesenchymal Stem Cell/Human Umbilical Vein Endothelial Cell Complexes.

Biogenic hydroxyapatite is known for its osteoinductive potential due to its similarity to human bone and biocompatibility, but insufficient vascularization compared to autogenous bone during early implantation limits bone integration and osteogenesis. Fluorine has been shown to improve hydroxyapatite's mechanical properties and the coupling of osteogenic and angiogenic cells. In this study, fluorine-modified biogenic hydroxyapatite (FPHA) with varying fluorine concentrations was prepared and tested for its ability to promote vascularized osteogenesis.

Optical sectioning methods in three-dimensional bioimaging.

In recent advancements in life sciences, optical microscopy has played a crucial role in acquiring high-quality three-dimensional structural and functional information. However, the quality of 3D images is often compromised due to the intense scattering effect in biological tissues, compounded by several issues such as limited spatiotemporal resolution, low signal-to-noise ratio, inadequate depth of penetration, and high phototoxicity. Although various optical sectioning techniques have been developed to address these challenges, each method adheres to distinct imaging principles for specific applications.

Advanced Al Hydrogel Propellants: Preparation, Thermal Stability, and Self-Sustainable Combustion Characteristics.

A new group of Al-based hydrogel propellants has been prepared and evaluated, aiming to replace currently used Al-ice propellants. The theoretical specific impulse () of these formulations is around 235 s. To enhance the ignition and combustion performances of these propellants, a 2% metastable intermixed composite Al@PVDF-CuO and minor ammonium perchlorate (AP) were introduced.

Network analysis of stress and stigma symptoms and their associations with quality of life among Chinese female infertility patients.

Background: This study has employed network analysis while investigating the interrelationships among stress and stigma symptoms and their associations with quality of life among Chinese female infertility patients.

Methods: In this cross-sectional study, 428 female patients who visited the Department of Reproductive Center of Dalian Women and Children's Medical Group with chief complaints of infertility symptoms were recruited using convenience sampling from November 2022 to December 2023. Fertility-related stress and stigma status were examined by the Fertility Problem Inventory (FPI) and Infertility Stigma Scale (ISS), respectively.

Application of botulinum toxin A in tissue repair and regeneration.

Tissue repair and regeneration present significant clinical challenges. Despite the array of treatments currently available in this domain, the urgent demand for innovative therapies persists, with the goal of enhancing patient quality of life. Recently, the application of botulinum neurotoxin type A (BoNT/A) has expanded within the realm of tissue repair and regeneration.

Clinical, pathological, and computed tomography morphological features of lung cancer with spread through air spaces.

Background: Spread through air spaces (STAS) is significantly associated with decreased overall survival (OS) and reduced recurrence-free survival. However, there are no reliable methods to confirm the presence of STAS before surgery. The sensitivity and specificity of the intraoperative frozen section diagnosis of STAS are not satisfactory.

Validation of the 2018 (New) ENMC Classification Criteria for Dermatomyositis in Chinese Patients with Idiopathic Inflammatory Myopathies.

Objectives: To validate the 2018 European Neuromuscular Centre classification (ENMC) criteria, compare its performance to the 1975 Bohan & Peter (B&P) and 2017 European League Against Rheumatism/American College of Rheumatology (EULAR/ACR) criteria for dermatomyositis (DM), and describe characteristics of different myositis-specific autoantibody (MSA)-positive patients defined by the ENMC-DM criteria.

Methods: Medical records and data on MSAs and muscle biopsies were retrospectively obtained from 1370 Chinese patients with idiopathic inflammatory myopathy (IIM) between 2008 and 2020. Patients were diagnosed with DM by at least two rheumatologists and classified according to the ENMC-DM, EULAR/ACR, and B&P criteria.

Electrically-driven drug delivery into deep cutaneous tissue by conductive microneedles for fungal infection eradication and protective immunity.

Fungal infections affect over 13 million people worldwide and are responsible for 1.5 million deaths annually. Some deep cutaneous fungal infections may extend the dermal barriers to cause systemic infection, resulting in substantial morbidity and mortality.