sRNA STnc3020 contributes to the virulence of Salmonella Typhimurium may via modulating the gene expression of prgJ of T3SS needle complex.

As important post-transcriptional regulators of gene expression, sRNAs play important modulatory roles in the environmental adaptation and virulence of bacteria. To investigate the regulatory role of sRNA STnc3020 in the virulence of Salmonella Typhimurium (S. typhimurium).

Long-Term Conservation Agriculture Improves Soil Quality in Sloped Farmland Planting Systems.

Conservation agriculture practices (CAs) are important under the increasingly serious soil quality degradation of sloping farmlands worldwide. However, little is known about how the long-term application of CAs influences soil quality at different slope positions. We conducted field experiments for a watershed sloping farmland's mainstream planting systems in the Three Gorges Reservoir area of China.

Response of Typical Shrubs Growth and Soil Nutrients to Graphene Addition in Impoverished Land of the Ulan Buh Desert.

Graphene can promote plant growth and improve soil conditions, but its effectiveness in enhancing infertile soils in arid regions remains unclear. This study selected three typical shrubs from the Ulan Buh Desert , , and as research subjects. Five graphene addition levels were set: 0 mg/L (C0), 25 mg/L (C1), 50 mg/L (C2), 100 mg/L (C3), and 200 mg/L (C4).

Rbbp6-Mediated Bmal1 Ubiquitination Inhibits YAP1 Signaling Pathway to Promote Ferroptosis in Diabetes-Induced Testicular Damage.

Background: Diabetes-induced testicular damage (DITD) is a common complication of diabetes. We investigated underlying mechanism of retinoblastoma-binding protein 6 (Rbbp6)-mediated brain and muscle ARNT-like 1 (Bmal1) ubiquitination in modulating ferroptosis in DITD.

Methods: Spermatogenic cell apoptosis and viability were measured by flow cytometry and cell counting kit 8 (CCK-8), respectively.

Discovery and characterization of temperature adaptation modules by mining L-glutamate decarboxylase derived from psychrophilic microorganisms.

Directed alterations of the enzyme's temperature adaptations, such as thermostability and optimal catalytic temperature (T), are usually the way to go in order to meet the applications, however they are limited by the understanding between protein structure and function. Glutamate decarboxylase (GADs) is a common enzyme in the food industry. In this study, we identified and characterized three novel L-glutamate decarboxylases from psychrophilic microorganisms.

Identification of novel drug targets for osteoarthritis by integrating genetics and proteomes from blood.

Background: Osteoarthritis (OA) is a degenerative osteoarticular disease, involving genetic predisposition. How the risk variants confer the risk of OA through their effects on proteins remains largely unknown. Therefore, we aimed to discover new and effective drug targets for OA and its subtypes.

Multi-method analysis revealed the mechanism of substrate selectivity in NHase: A gatekeeper residue at the activity center.

Recognizing the critical need to elucidate the molecular determinants of this selectivity offers a pathway to engineer enzymes with broader and more versatile catalytic capabilities. Through integrated methods including phylogenetic analysis, molecular docking, and structural analysis, we identified a pivotal amino acid residue, αTrp116, linking the substrate binding pocket and the active site of a NHase from Pseudonocardia thermophila JCM 3095 (PtNHase). This residue acts as a crucial determinant of substrate specificity within the NHase enzyme.

BRD7 facilitates ferroptosis via modulating clusterin promoter hypermethylation and suppressing AMPK signaling in diabetes-induced testicular damage.

Background: Diabetes mellitus (DM)-induced testicular damage is associated with sexual dysfunction and male infertility in DM patients. However, the pathogenesis of DM-induced testicular damage remains largely undefined.

Methods: A streptozotocin (STZ)-induced diabetic model and high glucose (HG)-treated in vitro diabetic model were established.

Prescription glucocorticoid medication and iridocyclitis are associated with an increased risk of senile cataract occurrence: a Mendelian randomization study.

Iridocyclitis and the use of glucocorticoid medication have been widely studied as susceptibility factors for cataracts. However, the causal relationship between them remains unclear. This study aimed to investigate the causal relationship between the development of iridocyclitis and the genetic liability of glucocorticoid medication use on the risk of senile cataracts occurrence by performing Two-sample Mendelian randomization (MR) analyses.

STM1863, a Member of the DUFs Protein Family, Is Involved in Environmental Adaptation, Biofilm Formation, and Virulence in Typhimurium.

Typhimurium (STM) is an important zoonotic Gram-negative pathogen that can cause infection in a variety of livestock and poultry. Meanwhile, as an important foodborne pathogen, the bacterium can survive in various stressful environments and transmits through the fecal-oral route, posing a serious threat to global food safety. To investigate the roles of STM1863, a member of the DUFs protein family, involved in STM environmental adaptation, biofilm formation, and virulence.

Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus in Xinjiang, China, from 2020 to 2022.

In recent years, the pig industry in Xinjiang, China, has been severely impacted by outbreaks of porcine epidemic diarrhea (PED), despite vaccination efforts. In this study, we investigated the genetic characteristics of currently prevalent porcine epidemic diarrhea virus (PEDV) strains in the region. We collected 548 samples from animals with suspected PED on large-scale pig farms in Xinjiang.

Preparation of TiCT modified rare earth doped PbO electrodes for efficient removal of sulfamethoxazole.

In this study, we deposited TiCT-modified, rare-earth-doped PbO on the surface of a carbon fabric via electrodeposition. The surface morphology and electronic structure of the electrode were characterized with SEM, XRD and XPS. The layered TiCT did not change the structure of β-PbO, and at the same time, it improved the crystallinity of the material and reduced the grains of PbO.

Luminescence properties and energy transfer of broadband NIR phosphor LiMgZrO: 1.0%Cr, y%Yb.

The discovery of high thermal stability, broad-band near-infrared (NIR) fluorescent phosphors holds significant potential in applications such as non-destructive testing, promoting plant growth, and night vision devices. In this study, a novel broad-band NIR phosphors LiMgZrO (LMZ): 1.0 %Cr, y%Yb were synthesized via a high-temperature solid-state reaction method, with the optimal doping concentration found to be y = 1.

Research on the Low-Temperature Impact Toughness of a New 100-mm Ultra-Thick Offshore Steel Fabricated Using the Narrow-Gap Laser Wire Filling Welding Process.

Ultra-thick offshore steel, known for its high strength, high toughness, and corrosion resistance, is commonly used in marine platforms and ship components. However, when offshore steel is in service for an extended period under conditions of high pressure, extreme cold, and high-frequency impact loads, the weld joints are prone to fatigue failure or even fractures. Addressing these issues, this study designed a narrow-gap laser wire filling welding process and successfully welded a 100-mm new type of ultra-thick offshore steel.

Graphene promotes the growth of Vigna angularis by regulating the nitrogen metabolism and photosynthesis.

Graphene has promising applications in agriculture and forestry. In the current study, six different concentrations of graphene (0mg/L, 0.01mg/L, 0.

, a trans-coding sRNA is involved in virulence modulation targeting mRNA in Typhimurium.

Typhimurium (STM) is a food-borne Gram-negative bacterium, which can infect humans and a wide range of livestock and poultry, causing a variety of diseases such as septicaemia, enteritis and abortion. We will decipher the impacts of sRNA on STM virulence and provide a theoretical basis to reveal the regulatory role and molecular mechanism of . The main objective of this study was to clarify whether sRNA exerts regulatory roles on STM pathogenicity.

Treatment outcomes and cognitive function following electroconvulsive therapy in patients with severe depression.

Background: Traditional treatments for major depressive disorder (MDD), including medication and therapy, often fail and have undesirable side effects. Electroconvulsive therapy (ECT) uses electrical currents to induce brief seizures in the brain, resulting in rapid and potent antidepressant effects. However, owing to misconceptions and controversies, ECT is not as widely used as it could and often faces stigmatization.