A protein fitness predictive framework based on feature combination and intelligent searching.

Machine learning (ML) constructs predictive models by understanding the relationship between protein sequences and their functions, enabling efficient identification of protein sequences with high fitness values without falling into local optima, like directional evolution. However, how to extract the most pertinent functional feature information from a limited number of protein sequences is vital for optimizing the performance of ML models. Here, we propose scut_ProFP (Protein Fitness Predictor), a predictive framework that integrates feature combination and feature selection techniques.

Rare and common species contribute disproportionately to alpine meadow community construction and functional variation.

It is widely accepted that rare species are the first species to become extinct after human-induced disturbances. However, the functional importance of rare species still needs to be better understood, especially in alpine meadow communities with harsher habitats, where the extinction rate of rare species may be higher. This study established a 1.

A pilot randomized clinical trial of γ-tocopherol supplementation on wood smoke-induced neutrophilic and eosinophilic airway inflammation.

Background: Air pollutants, including particulates from wood smoke, are a significant cause of exacerbation of lung disease. γ-Tocopherol is an anti-inflammatory isoform of vitamin E that has been shown to reduce allergen-, ozone-, and endotoxin-induced inflammation.

Objective: The objective of this study was to determine whether γ-tocopherol would prevent experimental wood smoke-induced airway inflammation in humans.

Zinc oxide nanoparticles enhanced rice yield, quality, and zinc content of edible grain fraction synergistically.

Zinc oxide nanoparticles (ZnO NPs) have been widely used in agriculture as a new type of Zn fertilizer, and many studies were conducted to evaluate the effect of ZnO NPs on plant growth. However, there are relatively few studies on the effects of application methods and appropriate dosages of ZnO NPs on rice yield, quality, grain Zn content, and distribution. Therefore, in the 2019 and 2020, field trials were conducted with six ZnO NPs basal application dosages of no ZnO NPs, 3.

Combining gamma-tocopherol and aspirin synergistically suppresses colitis-associated colon tumorigenesis and modulates the gut microbiota in mice, and inhibits the growth of human colon cancer cells.

Despite being shown to be effective for chemoprevention of colorectal cancer, aspirin has limitations including adverse effects and inability to block colitis-associated colon cancer (CAC). γ-Tocopherol (γT), a vitamin E form, has been reported to mitigate experimental colitis and CAC, prolong the anti-inflammatory activity of aspirin and alleviate aspirin-induced adverse effect. We therefore hypothesize that combining γT and aspirin is better than either compound singly for suppressing CAC.

The Role of AQ in the Regioselectivity of Strong Alkyl C-O Bond Activation Catalyzed by Pd(OAc): A Density Functional Theory Mechanistic Study.

A density functional theory method was employed to investigate the mechanism of C-O bond activation of butanoic acid substrates bearing the 8-aminoquinoline (AQ) group catalyzed by Pd(OAc). The whole reaction consists of five fundamental steps: the chelation of substrate , the C-H activation step, the C-N coupling step, the protodepalladation step, and the release of the final product. The calculated results indicated that the protodepalladation step is the rate-determining step with a free energy barrier of 24.

Synthesis of Alkenylboronates from -Tosylhydrazones through Palladium-Catalyzed Carbene Migratory Insertion.

The palladium-catalyzed oxidative borylation reaction of -tosylhydrazones has been developed. The reaction features mild conditions, broad substrate scope, and good functional group tolerance. It thus represents a highly efficient and practical method for the synthesis of di-, tri-, and tetrasubstituted alkenylboronates from readily available -tosylhydrazones.

Shade-induced nuclear localization of PIF7 is regulated by phosphorylation and 14-3-3 proteins in .

Shade avoidance syndrome enables shaded plants to grow and compete effectively against their neighbors. In , the shade-induced de-phosphorylation of the transcription factor PIF7 (PHYTOCHROME-INTERACTING FACTOR 7) is the key event linking light perception to stem elongation. However, the mechanism through which phosphorylation regulates the activity of PIF7 is unclear.