Regulation of β1-integrin in autophagy and apoptosis of gastric epithelial cells infected with Helicobacter pylori.

Helicobacter pylori infection is an essential factor in the development of human gastric diseases, but its pathogenic mechanism is still unclear. In this work we have showed that, the LC3II levels were increased and β1-integrin levels were decreased in H. pylori-positive human gastric tissue samples and H.

Polyethylene microplastics affect the distribution of gut microbiota and inflammation development in mice.

Environmental pollution caused by plastics has become a public health problem. However, the effect of microplastics on gut microbiota, inflammation development and their underlying mechanisms are not well characterized. In the present study, we assessed the effect of exposure to different amounts of polyethylene microplastics (6, 60, and 600 μg/day for 5 consecutive weeks) in a C57BL/6 mice model.

Thalassorhabdomicrobium marinisediminis gen. nov., sp. nov., a member of the family Hyphomonadaceae isolated from the Bohai Sea.

A novel Gram-stain-negative bacterium, designated strain BH-SD16, was isolated from a marine sediment sample collected in the Bohai Sea. Cells of strain BH-SD16 are aerobic, non-flagellated oval-shaped rods, showing oxidase- and catalase-positive activities. Growth occurs between 15-45 °C (optimum, 30 °C), at pH 6.

The regulatory role of C1q on Helicobacter pylori-induced inflammatory cytokines secretion in THP-1 cells.

C1q, as a LAIR-1 ligand, maintains monocytes quiescence and possess immunosuppressive properties. To understand the roles and molecular mechanisms, C1q mediated inflammation cytokines and several pivotal proteins in THP-1 cells after H. pylori infection were detected.

Mechanistic Insight Into the Interaction Between Urease Subunit α and Its Molecular Chaperone Hsp60.

is the etiologic agent in a variety of gastroduodenal diseases. As its key pathogenic factors, both urease and Hsp60 play important roles in the pathogenesis of . Previous studies have suggested that there is close relationship between urease and Hsp60, which implied that Hsp60 may act as a chaperone in urease stabilization and assembly.

Application of methylation in improving plasmid transformation into Helicobacter pylori.

Helicobacter pylori is an important gastrointestinal pathogen. Its strains possess different levels of powerful restriction modification systems, which are significant barriers to genetic tools used for studying the role of functional genes in its pathogenesis. Methylating vectors in vitro was reported as an alternative to overcome this barrier in several bacteria.

Application of FLP-FRT System to Construct Unmarked Deletion in and Functional Study of Gene in Pathogenesis.

is a Gram-negative, microaerophilic bacterium associated with human gastric diseases. Further investigations on virulence genes are still required to clarify the pathogenic mechanism of and the heterogeneous problem of infection. In order to develop an efficient and accurate method to study gene functions in pathogenesis, an unmarked deletion method for both a single gene and a large fragment was established based on the FLP-FRT recombination system.

The leukocyte-associated immunoglobulin (Ig)-like receptor-1 modulating cell apoptosis and inflammatory cytokines secretion in THP-1 cells after Helicobacter pylori infection.

Objective: Helicobacter pylori is a Gram-negative, microaerophilic bacteria usually found in the stomach, which may evade its host's immune system and present long-term symptoms in affected individuals. This study aimed to evaluate the functional role of leukocyte-associated immunoglobulin (Ig)-like receptor-1 (LAIR-1) in the strategies and underlying molecular mechanisms by which H. pylori escapes the host's immune responses.