RfxCas13d-mediated inhibition of alleviates renal fibrosis via PI3K/AKT signaling pathway.

Background: Circular RNAs () have been shown to be involved in the development of chronic kidney disease (CKD). This study aimed to investigate the role of in renal fibrosis, which is a hallmark of CKD.

Methods: In this study, we established a unilateral ureteral obstruction (UUO) model and delivered RfxCas13d knockdown plasmid into renal parenchymal cells retrograde injection through the ureter followed by electroporation.

Kaempferitrin attenuates unilateral ureteral obstruction-induced renal inflammation and fibrosis in mice by inhibiting NOX4-mediated tubular ferroptosis.

Tubular ferroptosis significantly contributes to renal inflammation and fibrosis, critical factors in chronic kidney disease (CKD). This study aims to investigate Kaempferitrin, a potent flavonoid glycoside from Bauhinia forficata leaves, renowned for its anti-inflammatory and antitumor effects, and to elucidate its potential mechanisms in mitigating inflammation and fibrosis induced by tubular ferroptosis. The study investigated Kaempferitrin's impact on tubular ferroptosis using a unilateral ureteral obstruction (UUO) model-induced renal inflammation and fibrosis.