Biological effect abundance analysis of hemolytic pathogens based on engineered biomimetic sensor.

Conventional pathogen detection strategies based on the molecular structure or chemical characteristics of biomarkers can only provide the "physical abundance" of microorganisms, but cannot reflect the "biological effect abundance" in the true sense. To address this issue, we report an erythrocyte membrane-encapsulated biomimetic sensor cascaded with CRISPR-Cas12a (EMSCC). Taking hemolytic pathogens as the target model, we first constructed an erythrocyte membrane-encapsulated biomimetic sensor (EMS).

Lantern-shaped flexible RNA origami for Smad4 mRNA delivery and growth suppression of colorectal cancer.

mRNA delivery has shown high application value in the treatment of various diseases, but its effective delivery is still a major challenge at present. Herein, we propose a lantern-shaped flexible RNA origami for mRNA delivery. The origami is composed of a target mRNA scaffold and only two customized RGD-modified circular RNA staples, which can compress the mRNA into nanoscale and facilitate its endocytosis by cells.

Surveillance of the Antimicrobial Susceptibility and Molecular Characteristics of Neisseria gonorrhoeae Isolates Collected in Changsha, China from 2016 to 2021.

Antibiotic treatment is critical for individuals infected with gonorrhea and preventing disease transmission. This study aimed to analyze the antimicrobial susceptibility and molecular epidemiological characteristics of Neisseria gonorrhoeae isolates in Changsha, China. A total of 271 N.

Surface environment complication makes Ag nanoclusters more robust and leads to their unique packing in the supracrystal lattice.

Silver nanoclusters have received unprecedented attention in cluster science owing to their promising functionalities and intriguing physical/chemical properties. However, essential instability significantly impedes their extensive applications. We herein propose a strategy termed "surface environment complication" to endow Ag nanoclusters with high robustness.

Polymorphism of Au(PR)Cl clusters: understanding C-H⋯π interaction and C-H⋯Cl-C van der Waals interaction on cluster assembly by surface modification.

The C-H⋯π interaction and the C-H⋯Cl-C van der Waals interaction play a crucial role in the crystallization of nanoclusters. In this paper, we present an example of a crystal system transformation of Au(PR)Cl from monoclinic (M) to trigonal (T) by surface modification. Atomically-resolved gold nanoclusters containing tris(4-chlorophenyl)phosphine and chloride ligands were synthesized and determined to be Au(-ClPPh)Cl (-ClPPh = tris(4-chlorophenyl)phosphine) by X-ray crystallography.

Emergence of ceftriaxone-resistant Neisseria gonorrhoeae strains harbouring a novel mosaic penA gene in China.

Objectives: The continuous emergence of ceftriaxone-resistant Neisseria gonorrhoeae strains threatens the effectiveness of current treatment regimens for gonorrhoea. The objective of the present study was to characterize three ceftriaxone-resistant N. gonorrhoeae strains with a novel mosaic penA allele isolated in China.

Identification of multidrug-resistant isolates with combined resistance to both ceftriaxone and azithromycin, China, 2017-2018.

The growing multidrug-resistant is a serious global threat to gonococcal therapy. During 2017-2018, we identified a rare multidrug-resistant (ceftriaxone and azithromycin) strain (GC250) and four strains (GC185, GC195, GC196 and GC249) with both resistance to ceftriaxone and decreased susceptibility to azithromycin. All strains belonged to NG-STAR ST1143, including the mosaic -60.

Rational construction of a library of M nanoclusters from monometallic to tetrametallic.

Exploring intermetallic synergy has allowed a series of alloy nanoparticles with prominent chemical-physical properties to be produced. However, precise alloying based on a maintained template has long been a challenging pursuit, and little has been achieved for manipulation at the atomic level. Here, a nanosystem based on M(S-Adm)(PPh) (where S-Adm is the adamantane mercaptan and M is Ag/Cu/Au/Pt/Pd) has been established, which leads to the atomically precise operation on each site in this M template.

Metal synergistic effect on cluster optical properties: based on Ag series nanoclusters.

Due to their high biological stability and strong fluorescence, thiolated metal nanoclusters have shown great potential as a new generation of bio-nano-materials. However, the ambiguous mechanism of fluorescence impedes the design and synthesis of highly fluorescent nanoclusters. In this work, Ag nanocluster and its dopants were chosen as a model to study the effect of metal synergy on the optical properties.

Total structural determination of [AuAg(Dppm)(SR)] comprising an open icosahedral AuAg core with six free valence electrons.

Herein, we report the first silver-rich nanocluster containing an open icosahedral Au1Ag12 core. This nanocluster is determined to be [Au1Ag24(Dppm)3(SR)17]2+ (where Dppm is short for bis-(diphenylphosphino)methane and SR is short for cyclohexyl mercaptan) by single-crystal X-ray diffraction and electrospray ionization mass spectrometry (ESI-MS). The Au1Ag24 consists of an open icosahedral Au1Ag12, which contains six free valence electrons surrounded by a big ring motif Ag12(Dppm)3(SR)15 and two SR groups.