Synthesis and in vitro cytotoxicity study of three di-organotin(IV) Schiff base di-acylhydrazone complexes.

Three di-organotin(IV) complexes have been synthesized by the reaction of Schiff base di-acylhydrazone ligands bis(5-chlorosalicylaldehyde) adipoylhydrazone and RSnCl [R = Me (1), Ph (2), n-Bu (3)]. Structures of all complexes were characterized by H, C, Sn NMR, elemental analysis, IR and mass spectrometry. Experimental results showed that the symmetric diacylhydrazone ligands coordinate the tin atom in a hexadentate form, where the tin atom shows a penta-coordination, in a distorted triangular bipyramid geometry.

Multi-pathway inducing ferroptosis by MnO-based nanodrugs for targeted cancer therapy.

A multifunctional nanodrug (Tf-DHA-ASO-MnO) based on manganese dioxide nanosheets was constructed by triple-dressing with transferrin, dihydroartemisinin, and antisense oligonucleotide sequences. Tf-DHA-ASO-MnO shows an effective targeted cancer therapy ability through the ferroptosis caused by the production of excessive lipid peroxides resulting from the combined effect of glutathione exhaustion, reactive oxygen species generation and down-regulation of glutathione peroxidase 4.

Genome-Wide Identification and Characterization of the Potato Family During Development and Stress.

Calmodulin-binding proteins belong to the IQ67 domain (IQD) gene family and play essential roles in plant development and stress responses. However, the role of gene family in potato ( L.) is yet to be known.

Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A.

Base editors (BEs) have been used to create C-to-T substitutions in various organisms. However, editing with rat APOBEC1-based BE3 is limited to a 5-nt sequence editing window and is inefficient in GC contexts. Here, we show that a base editor fusion protein composed of Cas9 nickase and human APOBEC3A (A3A-PBE) converts cytidine to thymidine efficiently in wheat, rice and potato with a 17-nucleotide editing window at all examined sites, independent of sequence context.

Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion.

Nucleotide base editors in plants have been limited to conversion of cytosine to thymine. Here, we describe a new plant adenine base editor based on an evolved tRNA adenosine deaminase fused to the nickase CRISPR/Cas9, enabling A•T to G•C conversion at frequencies up to 7.5% in protoplasts and 59.