Mechanistic elucidation of QiJu-DiHuang Wan in management of age-related dry eye through metabolomics and network pharmacology.

Background: QiJu-DiHuang Wan (QJDHW), a frequently employed Chinese herbal formula, is used to treat blurred vision. Even so, it is unclear how it works in treating age-related dry eyes.

Objective: The aim of this research is to explore the potential mechanisms of QJDHW in treating dry eye using UHPLC-QE-MS, metabolomics, and network pharmacology.

Effect of rapid rehabilitation nursing on improvement of quality of life after super-miniPCNL and risk analysis for postoperative complications.

Objective: To investigate the effect of rapid rehabilitation nursing on the improvement of quality of life after super-miniPCNL (SMP) and the risk analysis of postoperative complications.

Methods: The clinical data of 124 SMP patients who were admitted from February 2019 to February 2021 were analyzed retrospectively. Thereinto, 58 cases received routine nursing were regarded as the control group (CG), and 66 received rapid rehabilitation nursing were considered as the observation group (OG).

Highly efficient chloramphenicol degradation by UV and UV/H O processes based on LED light source.

In this study, UV-LED was employed as a novel light source to investigate the degradation of a representative antibiotic compound, chloramphenicol (CAP), in the absence or presence of H O . The UV-LED irradiation showed a higher capability for degradation of CAP than conventional UV-Hg vapor lamps. Effects of the initial CAP concentration, UV wavelength, and light intensity on the degradation of CAP by UV-LED were evaluated.

Detection of Brucellosis in Sika Deer ( Cervus nippon ) through Loop-mediated Isothermal Amplification (LAMP).

Brucellosis (Brucella bovis) in sika deer ( Cervus nippon ) can cause enormous losses to stag breeding, especially in areas in which stag breeding has become an important industry. It also poses a threat to humans because it is a zoonotic disease. Use of the loop-mediated isothermal amplification (LAMP) assay has been poorly described in the diagnosis of brucellosis in deer.

Shallow groundwater surrounding the Likeng landfill, Guangzhou, China - major ions and elements indicating the contamination sources.

An investigation of groundwater contamination around the Likeng landfill, Guangzhou, was carried out. Major ions and elements of 34 groundwater samples were measured, and the Piper trilinear diagram and expanded Durov diagram were used to analyze the chemical types and hydro-geochemical processes of the groundwater. End Member Mixing Analysis was used to find the types and sources of pollutants.

Comparative transcriptome analysis of Brucella melitensis in an acidic environment: Identification of the two-component response regulator involved in the acid resistance and virulence of Brucella.

Brucella melitensis, encounters a very stressful environment in phagosomes, especially low pH levels. So identifying the genes that contribute to the replication and survival within an acidic environment is critical in understanding the pathogenesis of the Brucella bacteria. In our research, comparative transcriptome with RNA-seq were used to analyze the changes of genes in normal-medium culture and in pH4.

Deep sequencing-based expression transcriptional profiling changes during Brucella infection.

Brucellosis is a worldwide zoonotic infectious disease that has significant economic effects on animal production and human health. The host macrophage -Brucella interaction is critical to the establishment of infections. Thus, the kinetic transcriptional profile of gene expression in macrophages infected with the Brucella melitensis strain 16M was investigated in the current study using a technology based on deep sequencing.

Subtractive screening with the Mycobacterium tuberculosis surface protein phage display library.

Surface proteins consist of secreted and membrane proteins and play a central role in the interaction of the pathogen with its environment, especially in the pathogenicity of Mycobacterium tuberculosis (MTB). Research on surface proteins in MTB has focused on 2D electrophoresis of culture filtrate proteins (CFP), extraction of transmembrane proteins with detergent and predicting their properties with a range of available algorithms. However, functional analysis of these secretomes is possible only if many proteins are expressed and purified individually, which limits a large number of studies to the function of the proteome.