Development and validation of a nomogram model to predict the risk of strangulated intestinal obstruction.

To develop and validate a nomogram model for discriminating simple intestinal obstruction and strangulated intestinal obstruction, thus providing objective evidence for clinical decision-making. Following pre-established inclusion and exclusion criteria, a retrospective analysis was conducted on the clinical data of 560 patients diagnosed with intestinal obstruction who were admitted to the Emergency Surgery Department of the First Affiliated Hospital of Anhui Medical University between January 1, 2020, and December 31, 2022. The data was subsequently split into a training cohort (n = 393) and a validation cohort (n = 167) using a 7:3 ratio.

Nanoparticle containing recombinant excretory/secretory-24 protein of enhanced the cellular immune responses in mice.

poses a global challenge as a parasite affecting small ruminants, yet the problem of absence of an effective vaccine against infection still exists. This investigation sought to appraise the immunological reaction induced by recombinant excretory/secretory-24 (rHcES-24) in combination with complete Freund's adjuvant (CFA) and bio-polymeric nanoparticles (NPs) within a murine model. In this study, rHcES-24 was encapsulated in poly(d, l-lactide-co-glycolide) (PLGA) and chitosan (CS) NPs, administered subcutaneously to mice.

Pilot-scale production of selenium-enriched Nostoc sphaeroides colonies and polysaccharides using two-phase cultivation strategy.

Nostoc sphaeroides, originally discovered in selenium-rich fields, is a photosynthetic, nitrogen-fixing cyanobacterium that forms edible spherical macrocolonies. However, prolonged selenium supplementation negatively impacts colony quality in culture. To address this, a two-phase cultivation strategy was developed to spatiotemporally separate biomass accumulation from selenium enrichment, resulting in high-quality selenium-enriched N.

Functional dissection of prenyltransferases reveals roles in endocytosis and secretory vacuolar sorting in type 2-ME49 strain of .

Prenyltransferases act essential roles in the prenylation modification, which is significant for proteins, like small GTPases to execute various important activities in (). The structures and partial functions of prenyltransferases (FTase, GGTase-I, and GGTase-II) in prenylation process have been dissected in . However, the cellular effects of prenyltransferases on type 2-ME49 strain of are largely unknown.

Evaluation of protective efficacy of recombinant Toxoplasma gondii DDX39 protein vaccine against acute and chronic T. gondii infection in mice.

Toxoplasma gondii, a pervasive parasite responsible for toxoplasmosis, poses significant health risks to humans and animals. In this study, we investigated the immunogenicity and protective efficacy of the recombinant T. gondii DDX39 protein formulated with ISA201 adjuvant (rTgDDX39) as a candidate vaccine against toxoplasmosis.

Partial Protection of Goats against Achieved with ADP-Ribosylation Factor 1 Encapsulated in PLGA Nanoparticles.

Background: (), a nematode with global prevalence, poses a major threat to the gastrointestinal health of sheep and goats. In an effort to combat this parasite, a nanovaccine was created using a recombinant ADP-ribosylation factor 1 (ARF1) antigen encapsulated within poly lactic-co-glycolic acid (PLGA). This study aimed to assess the effectiveness of this nanovaccine in providing protection against infection.

Lactobacillus helveticus attenuates alcoholic liver injury via regulation of gut microecology in mice.

Previous reports have demonstrated that alcohol consumption significantly reduces the abundance of Lactobacillus in the gut. In this study, we selected five species of the genus Lactobacillus, commonly found in fermented foods, and acknowledged them as safe, edible, and effective in preventing or treating certain diseases, to evaluate their effects on alcoholic liver disease (ALD). By comparing the liver damage indices in each group, we found that the type strain of Lactobacillus helveticus (LH, ATCC 15009) had the most marked alleviating effect on ALD-induced liver injury.

Engineering of Methionine Adenosyltransferase toward Mitigated Product Inhibition for Efficient Production of -Adenosylmethionine.

-Adenosylmethionine (SAM) is a crucial metabolic intermediate playing irreplaceable roles in organismal activities. However, the synthesis of SAM by methionine adenosyltransferase (MAT) is hindered by low conversion due to severe product inhibition. Herein structure-guided semirational engineering was conducted on MAT from (MAT) to mitigate the product inhibitory effect.

and discovery of antioxidant sea cucumber peptides with antineurodegenerative properties.

Since oxidative stress is often associated with neurodegenerative diseases, antioxidants are likely to confer protection against neurodegeneration. Despite an increasing number of food-derived peptides being identified as antioxidants, their antineurodegenerative potentials remain largely unexplored. Here, a sea cucumber peptide preparation - the peptide-rich fraction of <3 kDa (UF<3K) obtained by ultrafiltration from protein hydrolyzate - was found to protect PC12 cells and from neurodegeneration by reducing oxidative stress and apoptosis, demonstrating its and neuroprotective effects.

Separating spatial representations from polarity encoding in the processing of number and sequence stimuli in a four-way classification task.

Although the SNARC effect in the processing of most magnitude stimuli and sequence stimuli has been reported for the past 30 years, it remains unclear whether this effect is caused by the spatial representation or polarity encoding of stimuli. In the present study, we designed five experiments using a four-way classification task to evaluate the ability of spatial representation theory and polarity encoding theory to explain the SNARC effect in the processing of number and sequence stimuli. In all five experiments in the present study, stimuli (Experiments 1 and 4: four different Arabic numbers, Experiment 2: sequence stimuli, Experiment 3: ordinal sequences relevant to working memory, Experiment 5: Chinese characters without any implicit spatial information) were centrally presented.

Interfacial charge transfer in sheet NiP-FeP heterojunction to promote the study of electrocatalytic oxygen evolution.

The substantial expense associated with catalysts significantly hampers the progress of electrolytic water-based hydrogen production technology. There is an urgent need to find non-precious metal catalysts that are both cost-effective and highly efficient. Here, the porous NiP-FeP nanomaterials were successfully prepared by hydrothermal method, nickel foam as the base, iron nitrate solution as the caustic agent and iron source, and finally phosphating at low temperature.

The plant-like protein phosphatase PPKL regulates parasite replication and morphology in Toxoplasma gondii.

Background: The protozoan parasite Toxoplasma gondii encodes dozens of phosphatases, among which a plant-like phosphatase absent from mammalian genomes named PPKL, which is involved in regulating brassinosteroid signaling in Arabidopsis, was identified in the genome. Among the Apicomplexa parasites, T. gondii is an important and representative pathogen in humans and animals.

Dual-task interference: Bottleneck constraint or capacity sharing? Evidence from automatic and controlled processes.

This study investigated whether the interference between two tasks in dual-task processing stems from bottleneck limitations or insufficient cognitive resources due to resource sharing. Experiment 1 used tone discrimination as Task 1 and word or pseudoword classification as Task 2 to evaluate the effect of automatic versus controlled processing on dual-task interference under different SOA conditions. Experiment 2 reversed the task order.

Longitudinal gut fungal alterations and potential fungal biomarkers for the progression of primary liver disease.

Liver disease, a major health concern worldwide, is a serious and progressive disorder. Herein, we not only established a mouse model of DEN+CCl-induced primary liver disease but also collected clinical human samples to investigate longitudinal alterations in the gut mycobiome. As liver disease advanced, gut integrity was disrupted, and the mycobiota was disturbed in the mouse models.

Current Status and Prospects of Artificial Intelligence Technology Application in Oil and Gas Field Development.

Artificial intelligence technology will be increasingly applied in the oil and gas industry. The rapid development of artificial intelligence technology can solve problems such as high environmental sensitivity and complex production processes in the oil and gas industry. In recent years, emerging technologies represented by artificial intelligence have developed rapidly, assisting petroleum enterprises in digital transformation and intelligent upgrading.

Guest-Facilitated Heteroleptic Assembly of Helical Anionocages Enables Reversible Chirality Modulation.

We report a novel strategy for reversible modulation of the supramolecular chirality based on guest-facilitated heteroleptic assembly of helical anionocages. Two triple-stranded helical anionocages including a chiral cage 1 (A L ) and a crown ether functionalized achiral cage 2 (A L ) were synthesized by anion coordination of bis-monourea-based ligands and PhPO . Both cages exhibited favorable binding with tetraethylammonium TEA and cobaltocenium Cob (endo-guest, bound in the cavity).

PLGA-Encapsulated Antigen ES-15 Augments Immune Responses in a Murine Model.

is a gastrointestinal parasite that adversely impacts small ruminants, resulting in a notable reduction in animal productivity. In the current investigation, we developed a nanovaccine by encapsulating the recombinant protein rHcES-15, sourced from the excretory/secretory products of , within biodegradable poly (D, L-lactide-co-glycolide) (PLGA) nanoparticles (NPs). The development of this nanovaccine involved the formulation of PLGA NPs using a modified double emulsion solvent evaporation technique.

Abalone peptide increases stress resilience and cost-free longevity via SKN-1-governed transcriptional metabolic reprogramming in C. elegans.

A major goal of healthy aging is to prevent declining resilience and increasing frailty, which are associated with many chronic diseases and deterioration of stress response. Here, we propose a loss-or-gain survival model, represented by the ratio of cumulative stress span to life span, to quantify stress resilience at organismal level. As a proof of concept, this is demonstrated by reduced survival resilience in Caenorhabditis elegans exposed to exogenous oxidative stress induced by paraquat or with endogenous proteotoxic stress caused by polyglutamine or amyloid-β aggregation.

attenuated lipopolysaccharide-induced acute lung injury by modulating the gut microbiota and SCFAs in mice.

Gut microbiota are closely related to lipopolysaccharide (LPS)-induced acute lung injury (ALI). () maintains the intestinal barrier function and regulates the balance of reduced glutathione/oxidized glutathione. However, it may be useful as a treatment strategy for LPS-induced lung injury.

Transcriptomic and metabolomic analyses reveal the essential nature of Rab1B in Toxoplasma gondii.

Background: The protozoan parasite Toxoplasma gondii encodes a dozen Rab proteins, which are parts of the small GTPase superfamily and regulate intracellular membrane trafficking. Our previous study showed that depletion of Rab1B caused severe defects regarding parasite growth and morphological structure, yet early defects of endocytic trafficking and vesicle sorting to the rhoptry in T. gondii are not expected to have a strong effect.