Microbiomics and volatile metabolomics-based investigation of changes in quality and flavor of oat ( L.) silage at different stages.

Objective: This study aimed to analyze the fermentation quality, microbial community, and volatile metabolites of oat silage harvested at two different stages, while examining the correlation between microorganisms and volatile metabolites.

Methods: Oats were harvested at two growth stages (pre-heading [PRH] and post-heading [POH] stages), followed by 90 days of natural fermentation, with 6 replicates per treatment. Pre- and post-silage samples were randomly selected for nutrient composition, fermentation parameters, microbial population, and high-throughput sequencing analysis.

Optimization of Extraction Process and Dynamic Changes in Triterpenoids of from Different Medicinal Parts and Growth Periods.

In this study, the triterpenoids in the leaves of L.cv. Mengzao (LIM) were extracted via microwave-assisted ethanol extraction, and the optimum extraction conditions for triterpenoids were determined through single-factor experiments and the Box-Behnken method.

Effects of Different Types of LAB on Dynamic Fermentation Quality and Microbial Community of Native Grass Silage during Anaerobic Fermentation and Aerobic Exposure.

Silage of native grasses can alleviate seasonal forage supply imbalance in pastures and provide additional sources to meet forage demand. The study aimed to investigate the effects of (LP), (LB), and in combination with (PB) on the nutritional quality, fermentation quality, and microbial community of native grass silage at 2, 7, 15, and 60 days after ensiling and at 4 and 8 days after aerobic exposure. The results showed that dry matter content, crude protein content, the number of lactic acid bacteria, and lactic acid and acetic acid content increased and pH and ammonia nitrogen content decreased after lactic acid bacteria (LAB) inoculation compared with the control group (CK).

Influence of Cellulase or on the Ensiling Performance and Bacterial Community in Mixed Silage of Alfalfa and .

The objective of this study was to evaluate the effects of or cellulase on the fermentation characteristics and bacterial community of mixed alfalfa ( L., AF) and (LC) silage. The harvested alfalfa and were cut into 1-2 cm lengths by a crop chopper and they were thoroughly mixed at a ratio of 3/2 (wet weight).