[In vitro effect of seven anthelmintic agents against adult Clonorchis sinensis].

Objective: To observe the in vitro effect of praziquantel, tribendimidine, levamisole, artemether, artesunate, albendazole and mebendazole against adult Clonorchis sinensis.

Methods: Seventy rats infected with 50-100 C. sinensis metacercariae for 5-7 weeks were euthanized, and adult C.

[Effect of six anthelmintics in oral treatment of mice infected with Armillifer agkistrodontis nymphs].

Objective: To observe the effect of praziquantel, mebendazole, tribendimidine, ivermectin, artemether and dihydroartemisinin against Armillifer agkistrodontis nymphs harbored in mice.

Method: Thirty-five mice infected each with 40 eggs of Armillifer agkistrodontis for 25-37 weeks were divided into 10 groups (2-8 mice per group). Among them, nine groups were treated orally with praziquantel 500 mg/(kg x d) x 5 d or 500 my/(kg x d) x 14 d, mebendazole 300 mg/(kg x d)x 5 d, tribendimidine 300 mg/(kg x d) x 5 d, ivermectin 8-10 mg/(kg x d) x 3 d or 15 mg/(kg x d) x l4 d, artemether 400 mg/(kg x d) x 5 d and dihydroartemisinin 200 mg/(kg x d) x 5 d, respectively.

Comparative effect of mebendazole, albendazole, tribendimidine, and praziquantel in treatment of rats infected with Clonorchis sinensis.

The aim of the study is to understand the anti-Clonorchis sinensis properties of mebendazole and albendazole, and compare to praziquantel and tribendimidine. Two hundred and thirty rats were divided into five batches for experimental treatment. In four batches, each rat was infected orally with 50 or 100 C.

[Treatment of rats infected with Clonorchis sinensis using clinical administration regimens of tribendimidine, praziquantel and artesunate].

Objective: To evaluate the efficacy in treatment of Clonorchis sinensis-infected rats using the administration regimens of tribendimidine, artesunate and praziquantel applied in clinical treatment of clonorchiasis.

Methods: The doses of tribendimidine, artesunate and praziquantel used in clinical treatment of clonorchiasis were converted to the doses used in rats by the method of equal effective dose conversion among different animals, while the administration regimens of the drugs were designed basing on the regimens used in clinical trials. Thus, the following dose schedules were set up, i.

[Efficacy of tribendimidine and albendazole in treating mice infected with Trichinella spiralis].

Objective: To observe the efficacy of tribendimidine and albendazole against Trichinella spiralis in mice.

Methods: A total of 85 Kunming strain mice, infected orally with 100 T. spiralis larvae, was divided into 3 groups: group A (adult stage, 7 d after infection), group B (migrating larva stage, 15 d after infection), and group C (encapsulated larva stage, 35 d after infection).

[Therapeutic effect of tribendimidine, artesunate and praziquantel administered to hamsters infected with Clonorchis sinensis].

Objective: To observe the effect of tribendimidine, artesunate and praziquantel in treatment of hamsters (Mesocricetus auratus) infected with Clonorchis sinensis.

Methods: A total of 93 hamsters, each infected with 30 C. sinensis metacercariae, were treated intragastrically with above-mentioned drugs at a single dose.

The in vitro and in vivo effect of tribendimidine and its metabolites against Clonorchis sinensis.

The purpose of the study was to understand the in vitro and in vivo effect of tribendimidine (TBD) and its metabolites of p-(1-dimethylamino ethylimino)aniline (aminoamidine, deacylated amidantel, BAY d 9216, dADT), acetylated dADT (AdADT), terephthalaldehyde (TPAL), and terephthalic acid (TPAC) against adult Clonorchis sinensis. In in vitro test, the adults of C. sinensis were placed to each of the 24 wells of a Falcon plate and maintained in Hanks' balanced salt solution-20% calf serum.

[Effect of tribendimidine, artesunate, artemether and praziquantel, administered intragastrically at single, multiple or combined doses, to rats infected with Clonorchis sinensis].

Objective: To assess the efficacy of single, multiple or combined oral doses of tribendimidine, artesunate, artemether and praziquantel against Clonorchis sinensis in rats.

Methods: A total of 147 rats, each infected with 50 C. sinensis metacercariae, were used in experimental chemotherapy.

Comparison of mitochondrial cytochrome oxidase 1 DNA sequences from Necator americanus hookworms maintained for 100 generations in golden hamsters (Mesocricetus auratus) and hookworms from natural human infections.

The human hookworm Necator americanus was maintained through one hundred generations in the golden hamsters. The strain is now routinely maintained in laboratory hamsters through serial passage, and is the laboratory strain of choice for vaccine studies. Comparison of the mitochondrial cytochrome oxidase 1 (cox-1) sequences was shown previously to be useful for comparing the genetic structure of populations of N.

Necator americanus: maintenance through one hundred generations in golden hamsters (Mesocricetus auratus). I. Host sex-associated differences in hookworm burden and fecundity.

The human hookworm Necator americanus was maintained through one hundred generations in the golden hamster (Mesocricetus auratus). The parasite strain employed here was ultimately adapted to hamsters without the requirement for exogenous steroids or other immunosuppressive agents. Moreover, there was no requirement to use neonatal hamsters--successful infections were obtained in 9- to 10-week-old hamsters infected subcutaneously with 250 hookworm larvae.