Single-cell sequencing identifies inflammation-promoting fibroblast-neutrophil interaction in peri-implantitis.

Aim: To reveal the cellular composition and molecular environment of the periodontal and peri-implant inflammatory infiltrates through a single-cell sequencing technique, which may explain the pathological difference between these two diseases. A special focus was placed on the phenotypes and potential roles of neutrophils and fibroblasts in peri-implant/periodontal tissue immunity.

Materials And Methods: High-throughput single-cell transcriptomic profiling of peri-implant tissues from patients with peri-implantitis as well as periodontal tissues from patients with periodontitis and healthy donors was performed.

[Characteristics of Cadmium Concentration and Transport of in Farmland with Different Cadmium Pollution Degrees].

This study aimed to elucidate the cadmium (Cd) concentration and transport characteristics of in farmland with different Cd pollution degrees, so as to provide a reference basis for phytoremediation of Cd-contaminated farmland. The multi-point experiments in farmland with different Cd pollution degrees[(Cd) 0.32-38.

Effects of electroacupuncture on rats with cognitive impairment: An iTRAQ-based proteomics analysis.

Objective: The study explores the effects of electroacupuncture (EA) at the governing vessel (GV) on proteomic changes in the hippocampus of rats with cognitive impairment.

Methods: Healthy male rats were randomly divided into 3 groups: sham, model and EA. Cognitive impairment was induced by left middle cerebral artery occlusion in the model and EA groups.

Single-Cell RNA Sequencing Identifies New Inflammation-Promoting Cell Subsets in Asian Patients With Chronic Periodontitis.

Periodontitis is a highly prevalent chronic inflammatory disease leading to periodontal tissue breakdown and subsequent tooth loss, in which excessive host immune response accounts for most of the tissue damage and disease progression. Despite of the imperative need to develop host modulation therapy, the inflammatory responses and cell population dynamics which are finely tuned by the pathological microenvironment in periodontitis remained unclear. To investigate the local microenvironment of the inflammatory response in periodontitis, 10 periodontitis patients and 10 healthy volunteers were involved in this study.

[Remediation of Cadmium Contaminated Paddy Fields Using Soil Conditioners].

Four soil conditioners, SAMMNS, CCT01, Mineral, and Tebeigai were selected for this study. The effects of the four conditioners on soil pH, bulk density, organic matter, available nutrients, texture, microaggregates, Cd available in soil, and Cd content in brown rice were investigated using field-controlled cadmium tests conducted in cadmium-contaminated paddy fields in Pingxiang. The results showed that compared to the control, soil conditioners could increase pH, bulk density, and cation exchange capacity in soil.

[Analysis on the specificity of the relevant points along the governor vessel in chronic gastritis based on tenderness reaction and tenderness threshold].

Objective: To explore the specificity of the relevant points along the governor vessel in chronic gastritis through the synchronous observation of the tenderness reaction and tenderness threshold on the back along the governor vessel in the volunteers with chronic gastritis and make the comparison with healthy volunteers.

Methods: A chronic gastritis group and a healthy volunteer group were prepared, 30 cases in each one. Using synchronous comparison, the tenderness reaction at the point inferior to each of the spinous processes from T to L along the governor vessel was observed.

[Involvement of hippocampal miR-664-3p in electroacupuncture induced improvement of learning-memory ability of cerebral ischemia-reperfusion rats].

Objective: To explore the effect of electroacupuncture (EA) on learning-memory ability and its correlation with miR-664-3p levels in the hippocampus in cerebral ischemia-reperfusion injury (CI/RI) rats.

Methods: A total of 27 male SD rats were randomly divided into sham operation, CI/RI model and EA groups (＝9 in each group). The CI/RI model was established by occlusion of the middle cerebral artery (MACO) according to the modified Longa methods.

[Accumulation of Heavy Metals in Different Rice Varieties].

Field experiments were conducted on the heavy metal contents (Cd, Pb, Cr, inorganic As, and total Hg) of the 129 main cultivated rice varieties in southern China. We analyzed the effects of different varieties and types of rice on the absorption capacities of these heavy metals. The results showed that the Cd content in 35 brown rice of early rice was 0.

[Nitrification Activity and Autotrophic Nitrifiers in Long-term Fertilized Acidic Upland Soils].

Soil microcosm incubation, molecular ecology techniques including denaturing gradient gel electrophoresis and Illumina MiSeq high-throughput sequencing, and bioinformatics analysis were carried out to investigate the effect of long-term fertilization with chemical fertilizers (NPK) and organic manure (OM) on soil nitrification activity and the autotrophic nitrifying communities in acidic upland soils. No fertilization soil (CK) was the control. Relationships between soil nitrification activities, autotrophic nitrifying communities, and soil characteristics were further evaluated.

[Influences of biochar and nitrogen fertilizer on soil nematode assemblage of upland red soil].

The use of biochar as soil remediation amendment has received more and more concerns, but little attention has been paid to its effect on soil fauna. Based on the field experiment in an upland red soil, we studied the influences of different application rates of biochar (0, 10, 20, 30, 40 t · hm⁻²) and nitrogen fertilizer (60, 90, 120 kg N · hm⁻²) on soil basic properties and nematode assemblages during drought and wet periods. Our results showed that the biochar amendment significantly affect soil moisture and pH regardless of drought or wet period.

[Hydrogen sulfide removal by the combination of non-thermal plasma and biological process].

A bench scale system integrating a non-thermal plasma (NTP) unit with a biotricking filtration (BTF) unit for the treatment of gases containing hydrogen sulfide (H2S) was investigated. The additional use of the biotrickling filter to NTP reactor not only leads to the enhancement of hydrogen sulfide removal efficiency up from 83.4% to 90.

[Gaseous phenol removal in a bio-trickling filter].

The performance of a bio-trickling filter (BTF) for treatment of phenol, a model pollutant, was presented. Influences of factors on phenol removal efficiency were studied. The BTF exhibited a high removal efficiency for phenol.

[Mechanism and performance of styrene oxidation by O3/H2O2].

It can produce a large number of free radicals in O3/H2O2, system, ozone and free radical coupling oxidation can improve the styrene removal efficiency. Styrene oxidation by O3/H2O2 was investigated. Ozone dosage, residence time, H2o2 volume fraction, spray density and molar ratio of O3/C8H8 on styrene removal were evaluated.

[Effects of combined application of biochar and inorganic fertilizers on the available phosphorus content of upland red soil].

Aiming at the low content of available phosphorus in upland red soil of Southern China, this paper studied the effects of combined application of biochar and inorganic fertilizers on the available phosphorus and organic carbon contents and the pH of this soil. With the combined application of biochar and inorganic fertilizers, the soil physical and chemical properties improved to different degrees. As compared with the control, the soil pH and the soil organic carbon and available phosphorus contents at different growth stages of oil rape after the combined application of biochar and inorganic fertilizers all had an improvement, with the increments at bolting stage, flowering stage, and ripening stage being 16%, 24% and 26%, 23%, 34% and 38%, and 100%, 191% and 317% , respectively.