Corrigendum: Two small-molecule inhibitors of proliferation .

[This corrects the article DOI: 10.3389/fcimb.2023.

Two small-molecule inhibitors of proliferation .

Background: Toxoplasmosis caused by is a globally distributed zoonosis. Most infections appear asymptomatic in immunocompetent individuals, but toxoplasmosis can be fatal in fetuses and immunocompromised adults. There is an urgent need to research and develop effective and low-toxicity anti- drugs because of some defects in current clinical anti- drugs, such as limited efficacy, serious side effects and drug resistance.

[Cloning and expression of Toxoplasma gondii autophagy-related protein 3 gene and preparation of its polyclonal antibody].

Objective: To clone and express autophagy-related protein 3 (TgAtg3) gene of Toxoplasma gondii, and obtain the specific polyclonal antibody against TgAtg3.

Methods: TgAtg3 cDNA was inserted into prokaryotic expression vector pET28a. After identification, the constructed plasmid pET28a-TgAtg3 was transformed into E.

[Preparation and identification of polyclonal antibody against small peptides of beta-tubulin of Toxoplasma gondii].

The amino acid sequences of beta-tubulin from Toxoplasma gondii stains (GT1 and ME49) and human were aligned by ClustalW2 software. Based on the alignment result, the C-terminal peptides of beta-tubulin of T. gondii were artificially synthesized.

[Preparation and antigenicity analysis of recombinant aegyptin-like protein of Aedes albopictus].

Objective: To clone and express the aegyptin-like protein (alALP) encoding gene from Aedes albopictus salivary gland, and analyze its antigenicity.

Methods: The homology, secondary structure and antigen peptides of alALP and aegyptin protein (GenBank No. ABF18122.

[Preparation and application of the polyclonal antibody of Toxoplasma gondii autophagy protein 8 (TgAtg8)].

Objective: To prepare and evaluate specific-TgAtg8 polyclonal antibody.

Methods: The known Saccharomyces cerevisiae Atg protein sequences were used to identify Toxoplasma gondii homologous protein through bioinformatics analysis. TgAtg8 cDNA was amplified and cloned into prokaryotic expression vector pGEX-6p-1.

Development-specific differences in the proteomics of Angiostrongylus cantonensis.

Angiostrongyliasis is an emerging communicable disease. Several different hosts are required to complete the life cycle of Angiostrongylus cantonensis. However, we lack a complete understanding of variability of proteins across different developmental stages and their contribution to parasite survival and progression.

[A diffusion tensor magnetic resonance imaging study of age-related cerebral white matter diffusion anisotropy in normal human adult].

Objective: To investigate the correlation between cerebral white matter fraction anisotropy (FA) in normal human adults using the diffusion tensor magnetic resonance (MR) imaging (DTI).

Methods: Forty-five adults with normal cerebral white matter MRI findings in 3 age groups (n=15), namely 25 approximately 35 years (young), 45 approximately 55 years (middle-aged) and 65 years or above (elderly), underwent conventional MRI and diffusion tensor MR imaging. FA was measured in different regions of interest (ROIs) including the genu and splenium of the corpus callosum, posterior limb and anterior limb of the internal capsule, centrum semiovale, frontal white matter, thalamus and head of the caudate nucleus.