[Study on the chemical constituents in roots of Gentiana dahurica].

Objective: To systematically study the chemical constituents in the roots of Gentiana dahurica.

Methods: Various column chromatographic techniques were used for isolation and purification. The structures were elucidated on the basis of spectral data (UV, IR, MS, NMR) and identified by comparing with the authentic substance.

Changes of gentiopicroside synthesis during somatic embryogenesis in Gentiana macrophylla.

IN VITRO plant regeneration of Gentiana macrophylla Pall. and determination of gentiopicroside content during somatic embryogenesis are described in the present work. The highest percentage of embryogenic callus formation was observed in Murashige and Skoog (MS) medium supplemented with 1.

[Analysis of volatile constituents from Anemarrhena asphodeloides by GC-MS].

Objective: To study the chemical constituents of the volatile oil from the rhizome of Anemarrhena asphodeloides.

Method: The volatile oil was steam distillation. Chemical constituents were separated and analyzed by GC-MS.

[Studies on the chemical constituents in root of Gentiana macrophylla from Shaanxi].

Objective: To in vestigate the constituents in root of Gentiana macrophylla.

Method: Various column chromatographic techniques were used for isolation and purification of the principles. The structures were elucidated on the basis of spectral data (UV, IR, MS, 1H-, 13C-NMR etc.