Fluorescent probes for and quantification of hydrogen peroxide.

Hydrogen peroxide (HO) plays essential roles in redox signaling and oxidative stress, and its dynamic concentration is critical to human health and diseases. Here we report the design, syntheses, and biological applications of and for quantitative measurement of HO. Both probes were successfully applied to detect endogenous HO fluxes in living cells or zebrafish, and biological effects of multiple stress inducers including rotenone, arsenic trioxide, and starvation were investigated.