Clinical outcomes of doubled-suture Nice knot augmented plate fixation in the treatment of comminuted midshaft clavicle fracture.

Background: Free bone fragments were difficult to be fixed in many comminuted midshaft clavicle fractures, and the absence of cortical alignment in comminuted fractures had direct influence on the stability of fixation. This survey was performed to assess the efficacy of doubled-suture Nice knot augmented plate fixation in the treatment of comminuted midshaft clavicle fractures.

Methods: Between 2013 and 2018, all patients with comminuted midshaft clavicle fractures treated with doubled-suture Nice knot augmented plate fixation were retrospectively reviewed and included in this research.

[Pluripotential differentiation of QY1 bone marrow mesenchymal stem cell line].

Objective: To explore the ability of QY1 bone marrow mesenchymal stem cell (MSCs) line cells to differentiate into adipocytes, chondrocytes, osteoblasts, cardiac myocytes,vascular endothelial cells, and neural cells in vitro.

Methods: The QY1 cells at passage 5 were treated with the adipogenic medium, the chondrogenic medium and the osteogenic medium, 5-azacytidine, vascular endothelial growth factor and neural cell medium (revulsant 1 was 10 mmol/L beta-mercaptoethanol; revulsant 2 was 2%dimethylsulfoxide and 10(-8)mol/L dexamethasone) in culture respectively in vitro. The differentiated cells were identified by staining, immunohistochemistry and RT-PCR.

[Differentiation of QY1 bone marrow pluripotential mesenchymal stem cell line cells into cardiomyocytes and vascular endothelial cells in vitro].

Objective: To explore the differentiation potential of QY1 bone marrow mesenchymal stem cell (MSCs) line cells into cardiacmyocytes and vascular endothelial cells in vitro, to optimize the suitable conditions of MSCs differentiating into cardiomyocytes in vitro, and to examine the potentials of MSCs differentiating into cardiomyogenesis and vasculogenesis.

Methods: Specifically committed differentiation inductive medium was employed, including 5-azacytidine for cardiomyogenesis and vascular endothelial growth factor for vasculogenesis in culture respectively in vitro. The differentiated cells were identified by immunohistochemistry and molecular biology.

[Biological stability analysis of QY1 pluripotential mesenchymal stem cell line from Sprague Dawley rat bone marrow].

Objective: To explore the optimal culture conditions in vitro and the biological characterizations of the QY1 pluripotential mesenchymal stem cell (MSC) line from Sprague-Dawley rat bone marrow and to analyze the biological stability of this MSC line so as to provide an ideal cell model for the further differentiation and actual application.

Methods: The methods and technologies of cell biology and stem cell tissue engineering were used to purify MSCs. We determined the effects of morphology of cell proliferation, the time of change medium, growth curves, doubling time, adhesive rates, chromosome, culture conditions, and repeated frost on the biological characterizations of MSCs.

[Induction of committed differentiation from yolk sac stem cells to CFU-GM].

Objective: To set up a stable technology for the growth of yolk sac CFU-GM.

Methods: The effects of various inductive factors on the growth of yolk sac CFU-GM and the characteristic of colonies were investigated. In vitro agar culture method was used in this study.

[Effects of murine bone marrow endothelial cell conditioned medium on the growth of yolk sac hematopoietic stem cells and progenitors].

Objective: To investigate the effects of murine bone marrow endothelial cell conditioned medium (mBMEC-CM) on the growth of yolk sac hematopoietic progenitors.

Methods: The serum-free mBMEC-CM was obtained from subcultures of murine endothelial cell line derived from bone marrow which was established in our laboratory. The murine yolk sacs were harvested on day 8.

[Committed hematopoeitic differentiation of embryonic stem cell].

Objective: To explore a stable technology for inducing ES cell to committed hematopoietic differentiation.

Methods: The effects of various inductive factors on BLast Colony-Forming Cell (BL-CFC) were investigated. In vitro differentiative system for ES cells was employed in this study.