Gene expression profiling of mice liver tissue after intragastric administration of Chinese nutgall extract.

Kunming mice (male, weight 20 +/- 2g) were daily intragastric administration of Chinese nutgall extract (0.2 mL/10 g body weight, equal to 8 g Chinese nutgall material/1 kg body weight) for 30 days. Liver tissue mRNA were extracted from normal control group and Chinese nutgall treated group respectively, then were reversely transcribed to cDNA with dUTP labeled by different fluorescence (Cy3, Cy5) as hybridization probes.

Gene expression profile analyses of mice livers injured by Leigongteng.

Aim: To analyze the gene expression profiles of mice livers injured by Leigongteng and explore the relationship between the differentially expressed genes and liver damage.

Methods: The experimental mice were randomly divided into a control group and a liver-injured group in which the mice were administrated 33 mu gamma of triptolide/kg per day for 30 d. Liver mRNAs were extracted from animals in both groups and were reverse-transcribed to cDNA with dUTP labeled by different fluorescence (Cy3, Cy5) as hybridization probes.

[Changes of gene expression profiles in CCl4 injured liver of mice].

Aim: To study the gene expression profiles between the CCl4 injured liver and normal liver in mice, and to screen the differentially expressed genes that relate to liver injury by CCl4 on a large scale using cDNA microarrays.

Methods: Male Kunming strain mice were divided into two groups: one was control group and another was CCl4 injured liver group that was given 0.1% CCI4 oil solution ip at dose of 10 mL x kg(-1) every three days, totally for ten times.