Publications by authors named "Qi-ni Gan"
Objective: To investigate the significance of severe acute respiratory syndrome associated coronavirus (SARS-CoV)-X4 protein expression in lungs of patients with SARS.
Methods: Pathological features of the lungs from 4 SARS patients were examined and the expression of SARS-CoV-X4 protein in the lungs was evaluated with immunohistochemical staining using specific antibodies against protein X4.
Results: Microscopically, all lungs from 4 cases showed edema, erythrocyte and fibrin exudates in the alveoli, hyperplasia of alveolar epithelium, necrosis, hyaline membrane formation and fibroblast foci.
Background: The genome of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) includes sequences encoding the putative protein X4 (ORF8, ORF7a), consisting of 122 amino acids. The deduced sequence contains a probable cleaved signal peptide sequence and a C-terminal transmembrane helix, indicating that protein X4 is likely to be a type I membrane protein. This study was conducted to demonstrate whether the protein X4 was expressed and its essential function in the process of SARS-CoV infection.
View Article and Find Full Text PDF[Preparation, identification, and analysis on tissue chips of polyclonal anti-peptide antibody to chemokine-like factor 1].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
October 2004
Article Synopsis
- The study aimed to create a polyclonal anti-peptide antibody against CKLF1 and utilize it for examining CKLF1 expression and function.
- The researchers selected a 16 amino acid sequence from the C-terminal end of CKLF1 and immunized rabbits with this peptide to generate the antibody.
- Results showed that the antibody effectively recognized CKLF1 in various tissues and had significant staining differences between normal and cancerous rectal tissues, indicating its potential as a tool for CKLF1 detection.