Rational design and synthesis of triazene-amonafide derivatives as novel potential antitumor agents causing oxidative damage towards DNA through intercalation mode.

In this work, we rationally designed and synthesized two novel triazene-amonafide derivatives 2-(2-(diisopropylamino)ethyl)-5-(3,3-dimethyltriaz-1-en-1-yl)-1H-benzo[de]isoquinoline-1,3(2H)-dione (D-11) and 5-(3,3-diethyltriaz-1-en-1-yl)-2-(2-(diisopropylamino)ethyl)-1H-benzo[de]isoquinoline-1,3(2H)-dione (D-12) as potential antitumor agents. The DNA damage induced by the intercalation mode of D-11 (D-12) towards DNA was electrochemically detected through the construction of efficient biosensors. The consecutive processes of reversible redox of naphthylimide ring and irreversible oxidation of triazene moiety were elucidated on the surface of glassy carbon electrode (GCE) by CV, SWV, and DPV methods.

Synthesis and biological evaluation of aminobenzamides containing purine moiety as class I histone deacetylases inhibitors.

The aminobenzamide is selective to class I histone deacetylases (HDACs) and displays unique tight-binding/slow-off HDAC-binding mechanism. Herein, we report a series of 9-substituted purine aminobenzamides that selectively inhibit class I HDACs. The activities in vitro showed compound 9d exhibited 12 folds more potent than MS-275 against HDAC1 isoform and showed excellent inhibitory activity on cancer cells, including HCT-116, MDA-MB-231, K562 cell lines.

Research on Treatment of Oily Sludge from the Tank Bottom by Ball Milling Combined with Ozone-Catalyzed Oxidation.

Difficult separation of oil-solid phase and high fine content of the recovered oil were two problems in the treatment of oily sludge from the tank bottom by the hot water-based extraction process. To solve the problems, one technology with "ball milling + ozone-catalyzed oxidation" as the core was studied, and the process parameters of ball milling and ozone-catalyzed oxidation were respectively optimized. After ball milling treatment, the oil content of dry oily sludge decreased from 33.

The control of clonorchiasis in Guangdong province, southern China.

Oriental liver fluke disease (clonorchiasis), caused by Clonorchis sinensis, is endemic in 81 counties in Guangdong province, China. This study was conducted following increasing lack of clonorchiasis awareness and increased consumption of raw freshwater fish and raw fish porridge at home and restaurants, which has been attributed to improved living standards in China, and is considered to have led to a higher C. sinensis infection rate in the country.

Small-scale spatial analysis of intermediate and definitive hosts of Angiostrongylus cantonensis.

Background: Angiostrongyliasis is a food-borne parasitic zoonosis. Human infection is caused by infection with the third-stage larvae of Angiostrongylus cantonensis. The life cycle of A.

[The impurity profiling of paclitaxel and its injection by UPLC-MS/MS].

An ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS) method was developed to elucidate the impurity profiles of paclitaxel and paclitaxel injections from different Chinese pharmaceutical companies. The fragmentation patterns for paclitaxel and the related impurities were analyzed and summarized. To remove the interference from auxiliary materials, such as hydrogenated castor oil, paclitaxel was dissolved in ethanol for acid, base, peroxide, and light induced forced degradation analysis, which could produce all the impurities exist in the paclitaxel injection.

Risk factors for Enterobius vermicularis infection in children in Gaozhou, Guangdong, China.

Background: Enterobius vermicularis infection is a prevalent intestinal parasitic disease in children. In this study, we explored the epidemiological status and risk factors for E. vermicularis infection in children in southern China.

[Distribution and schistosomiasis transmission risks of Biomphalaria straminea in inland China].

Biomphalaria straminea, the intermediary host of Schistosoma mansoni, was found by survey in local areas of Shenzhen City in 1981, which was the first finding of the snail in inland China. By 2013, the snail had spread in large range of Shenzhen City and overspread to the surrounding regions, Dongguan City and Huizhou City. Due to the facts that Shenzhen City has many international communications and is a key area of labor export, with high population mobility, while the reports of S.

[Epidemiological analysis of malaria epidemic situation in Guangdong Province, 2011].

Objective: To analyze the features of malaria epidemic in Guangdong Province in 2011 so as to provide the evidence for the elimination of malaria in Guangdong Province.

Methods: The data from the Disease Reporting Information System of Chinese Center for Disease Control and Prevention were analyzed with the descriptive epidemiological method for the epidemiological characteristics of malaria in Guangdong Province in 2011.

Results: A total of 96 malaria cases were reported in Guangdong Province in 2011, with an annual mean incidence of 0.

[Methods for grain size analysis of nanomedicines].

As nanomedicines are developing fast in both academic and market areas, building up suitable methods for nanomedicine analysis with proper techniques is an important subject, requiring further research. The techniques, which could be employed for grain size analysis of nanomedicines, were reviewed. Several key techniques were discussed with their principles, scope of applications, advantages and defects.

[Analysis and evaluation of the impurity of felodipine and its tablets].

The paper reports the systematic study on felodipine and its impurities in tablets, to improve its quality standards for the control of the related substances. HPLC-DAD, UPLC-MS, IR and NMR methods were used for the isolation of felodipine and its impurities in tablets, their identification and the zebrafish animal model was used for the analysis of the toxic impurities. In felodipine material and its tablets, three impurities are isolated and identified.

High performance liquid chromatographic separation of thirteen drugs collected in Chinese Pharmacopoeia 2010(Ch.P2010) on cellulose ramification chiral stationary phase.

The enantiomers separation of thirteen drugs collected in Ch.P2010 was performed on chiral stationary phase of cellulose ramification (chiralpak OD and chiralpak OJ) by high performance liquid chromatographic (HPLC) methods, which included ibuprofen (C1), ketoprofen (C2), nitrendipine (C3), nimodipine (C4), felodipine (C5), omeprazole (C6), praziquantel (C7), propranolol hydrochloride (C8), atenolol (C9), sulpiride (C10), clenbuterol hydrochloride (C11), verapamil hydrochloride (C12), and chlorphenamine maleate (C13). The mobile phase consisted of isopropanol and n-hexane.

[Endemic situation of schistosomiasis in Guangdong Province from 2004 to 2009].

Objective: To understand the endemic situation of schistosomiasis in Guangdong Province.

Methods: The surveillance of Oncomelania hupensis snails and infectious sources was carried out in the former endemic areas, and the surveillance data were statistically analyzed from 2004 to 2009 according to the national and provincial surveillance protocols.

Results: From 2004 to 2009, an area of 927.

Selecting an appropriate interestingness measure to evaluate the correlation between Chinese medicine syndrome elements and symptoms.

To select the best interestingness measure appropriate for evaluating the correlation between Chinese medicine (CM) syndrome elements and symptoms, 60 objective interestingness measures were selected from different subjects. Firstly, a hypothesis for a good measure was proposed. Based on the hypothesis, an experiment was designed to evaluate the measures.

The TCM pattern of the six-zang and six-fu organs can be simplified into the pattern of five-zang and one-fu organs.

Objective: To simplify the TCM pattern of the zang-fu organs.

Methods: A database of zang-fu syndromes was established. The relationship between the zang-fu syndromes was analyzed by means of frequency analysis, association rule, and phi-coefficient correlation.

[Investigation on Angiostrongylus cantonensis infection in rodents in Guangdong Province].

Objective: To investigate the natural infection of Angiostrongylus cantonensis in Guangdong Province, and to provide the scientific evidence for control measures.

Methods: The investigation was carried out in 56 villages of 28 towns of 28 counties/districts in East Guangdong, West Guangdong, the mountain area of North Guangdong and Peal River Delta of the Province from 2005 to 2010. The rodents were captured with live trap and the species identified.

First provincial survey of Angiostrongylus cantonensis in Guangdong Province, China.

The rat lungworm Angiostrongylus cantonensis is a zoonotic nematode with a wide distribution. We report the first provincial survey of the prevalence of A. cantonensis infection among wild rodents and snails in Guangdong Province, China.

Aluminium-phosphorus interactions in plants growing on acid soils: does phosphorus always alleviate aluminium toxicity?

Aluminium (Al) toxicity and phosphorus (P) deficiency are considered to be the main constraints for crop production in acid soils, which are widely distributed in tropical and subtropical regions. Conventionally, P addition is regarded as capable of alleviating Al toxicity in plants. However, this field is still rife with unsubstantiated theories, especially for different plant species growing on acid soils.

[Fingerprints of Curcuma wenyujin by RP-HPLC].

Objective: To establish a mutual mode fingerprint of Curcuma wenyujin for quality control of C. wenyujin.

Methods: Waters Symmetry C18 column was used; the mobile phase was methanol-water in a linear gradient elution with the flow rate of 1.

Development and validation of HPLC-UV-MS method for the control of four anti-diabetic drugs in suspected counterfeit products.

An HPLC-UV method has been developed for the determination of valibose, miglitol, voglibose and acarbose, the four anti-diabetic drugs. The separation was accomplished successfully by using reversed phase chromatography (Prevail carbohydrate column, 250 mm x 4.6 mm, 5 microm) with a gradient acetonitrile-phosphate buffer solution (pH 8.

Nineteen clinical features of fever in Chinese medicine.

Based on the 4,067 descriptions about fever in the medical records of the well-known Chinese medicine physicians throughout the ages, 19 different clinical features of fever are summarized as follows: chilly fever, high fever, low fever, dull fever, bones fever, alternating chills and fever, tidal fever, head fever, face fever, chest fever, back fever, abdomen fever, waist fever, palms and soles fever, lower extremity fever, afternoon fever, evening fever, postpartum fever, and menstrual fever. Although, some of these fever features have not be paid close attention to in Western medicine, stage-division and classification about fever in Western medicine deserve to be used by Chinese medicine for reference.

[Raman spectroscopy fluorescence background correction and its application in clustering analysis of medicines].

During Raman spectroscopy analysis, the organic molecules and contaminations will obscure or swamp Raman signals. The present study starts from Raman spectra of prednisone acetate tablets and glibenclamide tables, which are acquired from the BWTek i-Raman spectrometer. The background is corrected by R package baselineWavelet.

[Survey on the focus of Angiostrongylus cantonensis in Guangdong Province].

Objective: To understand the distribution of Angiostrongylus cantonensis foci in Guangdong Province for making surveillance program.

Methods: Survey sites were chosen by strata sampling according to different geographic locations. Totally 22 survey sites were selected in four regions: East Guangdong, West Guangdong, North Guangdong and the Pearl River Delta.

[The apoptosis of mouse macrophage J774A.1 induced by oxysterol depend on NF-kappaB activation].

Aim: Investigated the relationship between NF-kappaB activation and cell apoptosis in mouse macrophages treated with 7-ketochesterol (7-KC).

Methods: Cell apoptosis was detected by MTT assay, DNA fragmentation assay and flow cytometric analysis. NF-kappaB activation was detected by western blot and immunohistochemistry.