[The effects of B7H4 on human bone marrow mesenchymal stem cell inhibiting proliferation of PHA activated T cells].

Objective: To investigate the effects of B7H4 on human bone marrow mesenchymal stem cells (HBMSC) mediating immune suppression.

Methods: The expression of the negative immunoregulatory factor B7H4 on HBMSC were analyzed by RT-PCR and flow cytometry (FCM), respectively. The blocking experiment was used to detect the effects of B7H4 on HBMSC mediating suppression on PHA induced T cell activation, proliferation and cell cycle.

[The up-regulated expression of LAIR-1 in tumor patients PBMC].

Aim: To investigate the expression of LAIR-1 in patients with tumors and the function of LAIR-1 in anticancer immunity.

Methods: A sandwich ELISA was employed to detect the serum sLAIR-1 from tumor patients and healthy individuals. The expression of LAIR-1 in CD4+ T cells, CD8+ T cells, NK cells and B cells isolated from the peripheral blood of cancer patients was examined by fluorescence staining and flow cytometry.

[Expression and biology identification of the human epididymis-specific gene ESC42 in E. coli].

Objective: To provide materials for the study of the function of ESC42 protein specifically expressed in the human epididymis.

Methods: The ESC42 gene was amplified from the human epididymis cDNA library by PCR and then cloned into prokaryotic expression vector pGEX-4T-1, expressed and purified by recombinant DNA techniques. The specificity of ESC42 protein was identified by Western blot and MALDI-TOF-MS.