[Raman Spectroscopy Measurement System of Dual Wavelength Laser Module].

Fluorescence interference is one of common interference factors during detection of Raman spectroscopy, while shifted-excitation Raman difference spectroscopy (SERDS) is an effective detection means to reject it. SERDS excites the test substance by two laser with different wavelengths, then difference the obtained Raman spectroscopies. SERDS can eliminate the fluorescence interference effectively, because the fluorescence backgrounds of the two spectroscopies are the same while the Raman peaks are translated.

Surface analysis using shell-isolated nanoparticle-enhanced Raman spectroscopy.

Surface-enhanced Raman scattering (SERS) is a powerful fingerprint vibrational spectroscopy with a single-molecule detection limit, but its applications are generally restricted to 'free-electron-like' metal substrates such as Au, Ag and Cu nanostructures. We have invented a shell-isolated nanoparticle-enhanced Raman spectroscopy (SHINERS) technique, using Au-core silica-shell nanoparticles (Au@SiO(2) NPs), which makes SERS universally applicable to surfaces with any composition and any morphology. This protocol describes how to prepare shell-isolated nanoparticles (SHINs) with different well-controlled core sizes (55 and 120 nm), shapes (nanospheres, nanorods and nanocubes) and shell thicknesses (1-20 nm).

Highly sensitive detection of silybin based on adsorptive stripping analysis at single-sided heated screen-printed carbon electrodes modified with multi-walled carbon nanotubes with direct current heating.

A new disposable multi-walled carbon nanotubes modified single-sided heated screen-printed carbon electrode (MWNT/ss-HSPCE) was fabricated. The electrochemical behavior of silybin was investigated by cyclic voltammetry and the probable electrode reaction mechanism was proposed. A simple and cheap direct current heating supplier was used to heating the electrode for adsorptive accumulation of silybin.

An extremely stable and sensitive end-column electrochemical detector based on heated copper microdisk electrode with direct current for CE and CE-Chip.

A heated copper microdisk electrode (HCME) was fabricated and successfully applied to capillary electrophoresis (CE) and CE-Chip as an electrochemical detector (ECD) for the detection of three carbohydrates and shikimic acid (SA) in Illicium verum Hook F., respectively. The temperature of HCME was heated by twin-wire-wound coil with direct current to reduce the magnetic interference.