[The effect of puerarin on prevention of oxidative damage of cultured lens capsule epithelial cells].

Objective: To investigate the peroxynitrite damage to the lens epithelial cells (LEC) and the prevention of this damage by puerarin in vitro.

Methods: This paper was experimental study. Rabbit LEC were isolated and cultured and the third or forth passage LEC were used in this experiment The experiment groups included: (1) CONTROL GROUP: Heat-pathogen free saline (NS) 200 microl was added to the medium; (2) ONOO- group: ONOO- 200 microl was added to obtain the terminal concentration at 0.

Puerarin decreases lens epithelium cell apoptosis induced partly by peroxynitrite in diabetic rats.

The present study was designed to observe if puerarin decreases lens epithelium cell (LEC) apoptosis induced partly by peroxynitrite (ONOO(-)). One hundred and eight rats were randomly divided into control group (n=36), streptozotocin (STZ) group (n=36) and STZ + puerarin group (n=36). The rats in the control group intraperitoneally (i.

The antagonism of cholecystokinin octapeptide-8 to the peroxynitrite oxidation on a diabetic cataractal rat model.

Background: Cataracts is considered be formed because of an abnormal glucose metabolic pathway or oxidative stress. We explored the damaging role of ONOO- and antagonism of cholecystokinin octapeptide-8 (CCK-8) in diabetic cataractal rat lenses.

Methods: A diabetic cataractal animal model was established by peritoneal injection of streptozotocine (STZ).

[Peroxynitrite-induced formation of diabetic cataract and its prevention by puerarin in rat].

Objective: To investigate the effect of peroxynitrite on the formation of diabetic cataract and its reversal by puerarin. in rat.

Methods: Normal Sprague-Dawley (SD) rats were equally divided into control group, streptozotocin group (STZ) and treatment group.