[Experimental research of discordant tracheal xenotransplantation].

Objective: To investigate the immunological rejection mechanism of tracheal xenotransplantation and xenografts as potential sources of trachea.

Methods: On SD rat model, a xenotransplanted tracheal from the guinea pig was established by wrapping it in the cervical muscles in situ. It was divided into cryopreserved group and uncryopreserved group.

[Pathological genomics of keloid fibroblastic cells].

Objective: Keloids result from the abnormal repair of the tissues after skin injuries where the pathological overgrowth of large and active fibroblastic cells expands beyond the boundaries of the initiating wound. Imbalanced expression of genes with an as yet unknown regulatory mechanism seems to result in the hypertrophic development of fibroblastic cells and over-productions of collagen. To get information as to genes which function in the actively growing keloid fibroblasts, we have applied a gene expression DNA-microarray technique by analyzing broad range of genes at once in a systematic fashion.

The earlier clinic experience of the reverse-flow anterolateral thigh island flap.

Since Song first reported the anterolateral thigh flap in 1984, this flap has been applied widely in clinical practice. In 1997, we found that the proximal end of the vascular pedicle of the anterolateral thigh flap showed active bleeding. We therefore believed that this flap could form the reverse-flow flap, which can be used clinically.

[Experimental research of promoting revascularization of tracheal transplantation by gene therapy].

Objective: To investigate methods of promoting revascularization of tracheal transplantation to increase the length of graft.

Methods: Transfer recombinant plasmid pcDNA3.1/myc-His(-)C-bFGF and pCD(2)-VEGF(121) into rabbit cervical muscle by direct injection of plasmid following electric pulses in vivo.