[Effects of three antigens extracted from Vibrio vulnificus on the immunological protection of Nibea albiflora].

Nibea albiflora was immunized by intraperitoneal injection with either Lipopolysaccharide (LPS) or outer membrane protein (OMP) extracted from Vibrio vulnificus or formalin killed Vibrio vulnificus (FKC). The influence of the three antigens on the immunological function of Nibea albiflora was determined at different time points following the injection by testing the agglutinating antibody titers of the serum, lysozyme activity of the serum, phagocytic activity of the blood and the relative survival percentage. The results showed that the three antigens have higher immunogenicity and antigenicity than the control group(injection with sterile saline).

[Polymorphisms of mitochondrial Cyt b gene and D-loop region in sweetfish (Plecoglossus altivelis Temminck et Schlegel) from Zhejiang and Fujian Provinces].

We reported here the molecular analysis of mitochondrial genomes in sweetfish (Plecoglossus altivelis). Thirty-one specimens were recruited from Ruian, Zhejiang Province, Ningde, Fujian Province, and Dongzhang reservoir, Fujian Province. DNA segments spanning cytochrome b gene and D-loop region derived from these specimens were PCR-amplified and subsequently characterized by direct DNA sequence analysis.