Integrative Single-Cell RNA-Seq and ATAC-Seq Analysis of Mouse Corneal Epithelial Cells.

Purpose: Corneal epithelial homeostasis is maintained by coordinated gene expression across distinct cell populations, but the gene regulatory programs underlying this cellular diversity remain to be characterized. Here we applied single-cell multi-omics analysis to delineate the gene regulatory profile of mouse corneal epithelial cells under normal homeostasis.

Methods: Single cells isolated from the cornea epithelium (with marginal conjunctiva) of adult mice were subjected to scRNA-seq and scATAC-seq using the 10×Genomics platform.

Auxiliary ligand-directed synthesis of a series of Cd(II)/Co(II) coordination polymers with methylenebis(3,5-dimethylpyrazole): syntheses, crystal structures, and properties.

Using a flexible methylenebis(3,5-dimethylpyrazole) ligand (H2MDP) with different aromatic carboxylate ligands, six new complexes {[Cd(H2MDP)(OPh)](H2O)}n (1), {[Cd(H2MDP)(MPh)](H2O)(1/10)}n (2), {[Cd(H2MDP)(PPh)](H2O)(3/2)}n (3), {[Co(H2MDP)(OPh)](H2O)2}n (4), {Co(H2MDP)(MPh)}n (5), {Co(H2MDP)(HBTC)}n (6) (H2OPh = phthalic acid, H2MPh = isophthalic acid, H2PPh = terephthalic acid, H3BTC = benzene-1,3,5-tricarboxylic acid) have been isolated under hydrothermal conditions. The crystal structure analyses reveal that complexes 1 and 4 feature two-dimensional undulated sheets with 4(4)·6(2) topology. Both complexes 2 and 3 contain M2L2-type metallocyclic motifs composed of two H2MDP ligands and two metal atoms, while complex 2 features a two-dimensional 4(4)·6(2) network constructed from binuclear cadmium units as four connected nodes, and complex 3 shows a two-dimensional network with 6(3)-hcb topology.

Two hybrid polyoxometalate-pillared metal-organic frameworks.

Two polyoxometalate-pillared 3D compounds, {Cu(5)(2-ptz)(6)(H(2)O)(4)(SiW(12)O(40))}·4H(2)O 1 and {Cu(9)(2-ptz)(12)(H(2)O)(6)(PMo(12)O(40))(2)}·H(2)O 2 (2-ptz = 5-(2-pyridyl)tetrazole) have been constructed based on different polyoxometalate anions, and copper-organic coordination polymer sheets by a hydrothermal method. Magnetic investigations reveal that both 1 and 2 exhibit antiferromagnetic coupling between the Cu(II) ions. Structural studies show the compound 1 exhibits a typical pcu-type net with the Schälfli symbol of {4(12)·6(3)}, and that compound 2 is a (3,4,6)-connected framework with novel {4(4)·6(10)·10}{6(3)}(2){6(5)·8} topology which has not been reported to date.

PH-controlled construction of Cu(I) coordination polymers: in situ transformation of ligand, network topologies, and luminescence and UV-vis-NIR absorption properties.

Two new complexes [Cu(I)(3)(L1)I(3)](n) (1, L1 = 2,5-bis(4-pyridyl)-1,3,4-oxadiazole) and [Cu(I)(3)(L2)I(2)](n) (2, L2 = 2,5-bis(4-pyridyl)-1,2,4-triazolate) are controllably formed by using aqueous ammonia to regulate the pH value of the reaction involving CuI and L1. Interestingly, L2 of 2 is in situ generated from the ring transform of L1 when increase the pH value of the reaction. 1 exhibits 2-D layer, while 2 shows 3-D MOFs with a novel 3-nodal 4,4,5-connected net topology of an unprecedented Point (Schlafli) symbol: (4·5(2)·6(2)·7)(5(4)·8(2))(4(3)·5·6(6)).

Therapeutic effect of transcatheter arterial chemoembolization and percutaneous injection of acetic acids on primary liver cancer.

Background: The resection rate of primary liver tumor in China is only about 20%. A lot of patients with moderate and advanced liver tumor may lose the chance of operation. The objective of present research was to study the efficacy of transcatheter arterial chemoembolization (TACE) combined with percutaneous injection of chemical agents and acetic acids in the treatment of patients with primary liver cancer (PLC).