SUMOylation and deacetylation affect NF-κB p65 activity induced by high glucose in human lens epithelial cells.

Aim: To explore the effects of IκBα SUMOylation and NF-κB p65 deacetylation on NF-κB p65 activity induced by high glucose in cultured human lens epithelial cells (HLECs).

Methods: HLECs (SRA01/04) were cultured with 5.5, 25, and 50 mmol/L glucose media for 24h, and with 50 mmol/L glucose media for 0, 12, and 24h respectively.

Comparison of 25 MHz and 50 MHz ultrasound biomicroscopy for imaging of the lens and its related diseases.

Aim: To compare the results of 25 MHz and 50 MHz ultrasound biomicroscopy (UBM) regarding the image characteristics of the lens and its related diseases and to discuss the application value of 25 MHz UBM in ophthalmology.

Methods: A total of 302 patients (455 eyes) were included in this study from November 2014 to May 2015. Patient ages ranged from 5 to 89y (mean±SD: 61.

miR-211 promotes lens epithelial cells apoptosis by targeting silent mating-type information regulation 2 homolog 1 in age-related cataracts.

Aim: To detect the expression of miR-211 in age-related cataract tissue, explore the effects of miR-211 on lens epithelial cell proliferation and apoptosis, and identify its target gene.

Methods: This study used real-time quantitative polymerase chain reaction (RT-qPCR) to measure the expression of miR-211 and its predicted target gene [silent mating-type information regulation 2 homolog 1 (SIRT1)] in 46 anterior lens capsules collected from age-related cataract patients. Human lens epithelial cell line (SRA01/04) cells were transfected with either miR-211 mimics, mimic controls, miR-211 inhibitors or inhibitor controls, 72h after transfection, miRNA and protein expression of SIRT1 were measured using RT-qPCR and Western blotting; then cells were exposed to 200 µmol/L HO for 1h, whereupon cell viability was measured by MTS assay, caspase-3 assay was performed.

miR-24-p53 pathway evoked by oxidative stress promotes lens epithelial cell apoptosis in age-related cataracts.

MicroRNA-24 (miR-24) serves an important role in cell proliferation, migration and inflammation in various types of disease. In the present study, the biological function and molecular mechanism of miR‑24 was investigated in association with the progression of age‑associated cataracts. To the best of our knowledge the present study is the first to report that the expression of miR‑24 was significantly increased in human anterior lens capsules affected by age‑associated cataracts as well as lens epithelial cells (LECs) exposed to oxidative stress.

Prevalence and risk factors of lens opacities in rural populations living at two different altitudes in China.

Aim: To investigate the prevalence of and risk factors for lens opacities in populations living at two different altitudes in China.

Methods: A total of 813 subjects aged ≥40y in Lhasa (Tibet Autonomous Region, China. Altitude: 3658 m) and Shaoxing (Zhejiang Province, China.

Sensitivity and Specificity of Diffusion-Weighted Magnetic Resonance Imaging in Diagnosis of Bladder Cancers.

Purpose: Our study is designed to examine the diagnostic performance of diffusion-weighted magnetic resonance imaging (DW-MRI) for bladder cancers (BC), and to determine whether DW-MRI can differentiate muscle invasive bladder cancer (MIBC) from non-MIBC (NMIBC).

Methods: A meta-analysis was performed of published studies that investigated the performance of DW-MRI for BC. These studies were retrieved from scientific literature databases using sensitive electronic search strategies.

HIF-1α SUMOylation affects the stability and transcriptional activity of HIF-1α in human lens epithelial cells.

Purpose: High blood glucose can induce oxidative damage and result in diabetic cataract. Oxidative stress induces various signal pathways including HIF-1α transcriptional signal to attenuate the damage of lenses. Whether HIF-1α SUMOylation can increase the activation of HIF-1α or if high glucose can affect the SUMOylation of HIF-1α in cultured human lens epithelial cells (HLECs) is still unknown, as well as the function of HIF-1α SUMOylation in oxidative damage induced by high glucose in HLECs.

AGEs Promote Oxidative Stress and Induce Apoptosis in Retinal Pigmented Epithelium Cells RAGE-dependently.

Advanced glycation end products (AGEs) are extremely accumulated in diabetes mellitus, particularly in retinal vascular and epithelium cells, and are confirmed to contribute to diabetic retinopathy (DR). In the present study, we determined the promotion by AGEs to the oxidative stress and mitochondrial dysfunction in retinal pigmented epithelium ARPE-19 cells and investigated the influence by the knockdown or the overexpression of receptor for AGEs (RAGE) on the AGE-promoted oxidative stress and mitochondrial dysfunction. Furthermore, we determined the induction by AGEs to the cell apoptosis and to the activation of B-cell lymphoma 2 (Bcl-2) families in the AGE-BSA-induced apoptosis, and examined the RAGE-dependence in such induction.

[Pathogens distribution and drug sensitivity of infantile dacryocystitis].

Objective: To investigate the pathogens and drug tolerance of infantile dacryocystitis in order to provide evidence for clinical drug use.

Methods: 230 cases of infant dacryocystitis (aged from 2 to 10 months, average 90 days) were analyzed for bacterial culture and drug sensitivity tests. These tests were performed following National Guide to Clinical Laboratory Procedures.

Measurement of retinal nerve fiber layer thickness in optic atrophy eyes of patients with optic neuritis using optical coherence tomography.

Objective: To measure the retinal nerve fiber layer (RNFL) thickness using optical coherence tomography (OCT) in optic atrophy eyes of patients with optic neuritis and investigate the correlation between the RNFL thickness and the visual function.

Methods: To compare the RNFL thickness using StratusOCT, three groups of the subjects were enrolled, including 72 patients with optic atrophy with definite demyelinating optic neuritis history (the neuritis group), 47 patients with advanced POAG atrophic neuropathy (the POAG group), and 47 healthy subjects (the control group). The correlation between the RNFL thickness and visual function parameters were investigated in the neuritis group, including the best-corrected visual acuity (BCVA), the visual field mean deviation (MD), pattern standard deviation (PSD), and P(100) latency of visual evoked potentials (VEP).

Fibronectin and focal adhesion kinase small interfering RNA modulate rat retinal Müller cells adhesion and migration.

Retinal Müller cells (RMCs) hypertrophy and proliferation play a crucial role in epiretinal membrane formation. This study was designed to analyze the effects of Fibronectin and specific FAK siRNA in cell adhesion and migration in rat Müller cells. RMCs were cultured and identified by GFAP, Vimentin, and GLAST mAb, respectively.

[UV-induced DNA damage and protective effects of antioxidants on DNA damage in human lens epithelial cells studied with comet assay].

Objective: To investigate the mechanism of UV-induced DNA damage and repair and the protective effects of antioxidants on DNA damage in human lens epithelial cells.

Methods: Human lens epithelial cells were irradiated at UV-doses 0.0 (control group), 2.

[Relationship between pterygium onset and ultraviolet rays exposure time].

Objective: To investigate the dose-effect relationship between the radiation of ultraviolet ray and the onset of pterygium quantitatively.

Methods: Interrogation was conducted to 95 patients with pterygium in Yacheng district, Sanya City, Hainan Province, 37 males and 58 females, aged 55.7 +/- 13.