Proximity-enhanced cysteine-histidine crosslinking for elucidating intrinsically disordered and other protein complexes.

Disordered proteins and domains are ubiquitous throughout the proteome of human cell types, yet the biomolecular sciences lack effective tool compounds and chemical strategies to study this class of proteins. In this context, we introduce a novel covalent tool compound approach that combines proximity-enhanced crosslinking with histidine trapping. Utilizing a maleimide-cyclohexenone crosslinker for efficient cysteine-histidine crosslinking, we elucidated the mechanism of this dual-reactive tool compound class.

Screening key genes for intracranial aneurysm rupture using LASSO regression and the SVM-RFE algorithm.

Background: Although an intracranial aneurysm (IA) is widespread and fatal, few drugs can be used to prevent its rupture. This study explored the molecular mechanism and potential targets of IA rupture through bioinformatics methods.

Methods: The gene expression matrices of GSE13353, GSE122897, and GSE15629 were downloaded.

Development and evaluation of the recombinant BP26 protein-based C-ELISA for human brucellosis diagnosis.

Introduction: Timely and accurate diagnosis is crucial for the effective treatment and prevention of brucellosis. Current serological diagnostics, primarily based on lipopolysaccharide (LPS), suffer from cross-reactivity with other Gram-negative bacteria, which limits their specificity. Periplasmic protein 26 (BP26), a highly immunogenic antigen found in , has emerged as a promising alternative for enhancing diagnostic specificity.

Coupling Study of Fracture Propagation-Filtration-Seepage during Hydraulic Fracturing Assisted Oil Displacement in Offshore Low-Permeability Reservoirs.

Offshore low-permeability reservoirs are mainly composed of complex fault-block structures with poor physical properties, which makes establishing an effective displacement relationship particularly challenging. Hydraulic fracturing assisted oil displacement (HFAD) can effectively increase the oil production of a single well by creating fractures to replenish the producing energy. In this study, the Khristianovich-Geertsma-de Klerk (KGD) model is used to calculate the propagation of vertical fractures, and the flow tube method is used to calculate the two-phase oil-water flow in filtration and seepage.

Review of osteokines in spinal cord injury: potential biomarkers during rehabilitation.

After spinal cord injury (SCI), mechanical unloading, denervation, as well as negative changes in blood supply, inflammation state, and hormone levels produce significant negative effects on bone density, leading to a high prevalence of osteoporosis after SCI. It has been recently discovered that skeletal bone also has endocrine functions. Osteokines, secreted from bone tissue, could play multiple roles in regulating bone density, muscle mass, glucose metabolism, and functions of the central nervous system-changes in the osteokine levels after SCI have been detected.

Anion-π Interactions on Functionalized Porous Aromatic Cages for Gold Recovery from Complex Aqueous with High Capacity.

High capacity, selective recovery and separation of precious metals from complex aqueous solutions is essential but remains a challenge in practical applications. Here, we prepared a thiophene-modified aromatic porous organic cage (T-PAC) with high stability for precise recognition and recovery of gold. T-PAC exhibits an outstanding gold uptake capacity of up to 2260 mg/g with fast adsorption kinetics and high adsorption selectivity.

Engineered Microneedle System Enables the Smart Regulation of Nanodynamic Sterilization and Tissue Regeneration for Wound Management.

The healing of bacterial biofilm-infected wounds is a complex process, and the construction of emerging therapeutic modalities that regulate the microenvironment to magnify therapeutic effects and reduce biotoxicity is still highly challenging. Herein, an engineered microneedle (MN) patch is reported to mediate the efficient delivery of black phosphorus nanosheets (BP NSs) and copper peroxide nanodots (CP NDs) for dual nanodynamic sterilization and methicillin-resistant staphylococcus aureus (MRSA)-infected wound healing. Results demonstrate that the system can eliminate biofilm, reduce cytotoxicity, promote angiogenesis and tissue regeneration by the multiple advantages of chemodynamic therapy (CDT), enhanced photodynamic therapy (PDT), and improved degradation process from BP NSs to phosphate for promoting cell proliferation.

Enhanced second harmonic generation in a 2 polymorph of CHNOZn for UV nonlinear optical applications.

The quest for novel nonlinear optical (NLO) polymorphs is pivotal for advancing laser technology and frequency conversion applications. We present a detailed study on the synthesis and properties of the 2 polymorph of CHNOZn, which exhibits an enhanced second harmonic generation (SHG) effect, 0.3 times that of KDP, nearly 8-fold higher than the 422 phase.

Advancement of 3D biofabrication in repairing and regeneration of cartilage defects.

Three-dimensional (3D) bioprinting, an additive manufacturing technology, fabricates biomimetic tissues that possess natural structure and function. It involves precise deposition of bioinks, including cells, and bioactive factors, on basis of computer-aided 3D models. Articular cartilage injurie, a common orthopedic issue.

Improving sulforaphane content in broccoli sprouts by applying Se: transcriptome profiling and coexpression network analysis provide insights into the mechanistic response.

Sulforaphane (SF) is a sulfur (S)-containing isothiocyanate found in cruciferous vegetables and is known for its potent anticancer properties. Broccoli sprouts, in particular, are considered safe and healthy dietary choices due to their high SF content and other beneficial biological activities, such as enhanced metabolite ingestion. The application of selenium (Se) is an excellent approach to enhance the abundance of SF.

Data-model interactive Rul prediction of stochastic degradation devices with multiple uncertainty quantification and multi-sensor information fusion.

This paper proposes an improved remaining useful life (RUL) prediction method for stochastic degradation devices monitored by multi-source sensors under data-model interactive framework. Firstly, the interrelationships among sensors are established using k-nearest neighbor (KNN), and the composite health index (CHI) is constructed by aggregating the multi-source sensor information through the graph convolutional network (GCN). Secondly, a stochastic degradation model with triple uncertainty at any initial degradation level is established to improve the matching degree between the stochastic degradation model and the actual degradation process.

The MYB-bHLH-NRAMP module modulates the cadmium sensitivity of quinoa by regulating cadmium transport and absorption.

Cadmium (Cd) is one of the most dangerous environmental pollutants and is easily absorbed by food crops. Quinoa is a kind of coarse grain crop with rich nutrition and strong stress resistance, which is easy to accumulate Cd. The increasingly serious soil Cd pollution poses a serious threat to the food safety of quinoa.

Efficacy and safety of anticoagulation in asymptomatic cirrhotic patients with portal vein thrombosis: a systematic review and meta-analysis.

Background: The role of anticoagulation in asymptomatic cirrhotic patients with portal vein thrombosis (PVT) remains unclear. This study aims to evaluate the efficacy and safety of anticoagulation in this patient population.

Methods: We systematically searched PubMed, Web of Science, Cochrane Library, and Embase up to August 2024.

Size Effect on Shear Behaviors of 3D-Printed Soft Rock-like Materials Under Different Shear Velocities.

The shear failure of rock masses is one of the primary causes of underground engineering instability. The shear mechanical behavior of rocks at different sizes is of great significance for studying the shear failure pattern of engineering rock masses. However, due to the presence of various joints and defects in natural rocks, the obtained rock specimens exhibit significant discreteness, making it difficult to customize specimen sizes for size effect studies.

Two novel lanthanide metal-organic frameworks based on tetraphenylethylene for ultra-high proton conduction.

Two novel isostructural anionic lanthanide metal-organic frameworks, (MeNH)[Ln(HTCBPE-F)·(HCOO)·DMF]·4.5DMF·2HO (Eu-MOF and Dy-MOF), based on tetraphenylethylene carboxylate ligands were successfully constructed and characterized. These two MOFs possess porous structures and water stabilities with uncoordinated carboxylate groups and dimethyl ammonium cations, which allow for high proton conductivities (5.

Providing an e-cigarette starter kit for smoking cessation and reduction as adjunct to usual care to smokers with a mental health condition: findings from the ESCAPE feasibility study.

Background: Smoking rates in the UK have declined steadily over the past decades, masking considerable inequalities, as little change has been observed among people with a mental health condition. This trial sought to assess the feasibility and acceptability of supplying an electronic cigarette (e-cigarette) starter kit for smoking cessation as an adjunct to usual care for smoking cessation, to smokers with a mental health condition treated in the community, to inform a future effectiveness trial.

Methods: This randomised controlled feasibility trial, conducted March-December 2022, compared the intervention (e-cigarette starter kit with a corresponding information leaflet and demonstration with Very Brief Advice) with a 'usual care' control at 1-month follow-up.

Efficacy of Ulinastatin in the Treatment of COVID-19: A Retrospective Study.

Purpose: This retrospective study aimed to evaluate the efficacy of ulinastatin in the treatment of COVID-19 patients compared to conventional therapy.

Patients And Methods: A total of 437 COVID-19 patients admitted to the Respiratory Oncology Department of our hospital between December 31, 2022, and July 8, 2023, were included in the study. Patients were classified into the observation group (n=62) receiving ulinastatin in addition to standard treatment and the control group (n=347) receiving standard treatment only.

Correction: Zhang et al. Astaxanthin Alleviates Early Brain Injury Following Subarachnoid Hemorrhage in Rats: Possible Involvement of Akt/Bad Signaling. 2014, , 4291.

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In-Situ Growth of Metallocluster Inside Heterometal-Organic Cage to Switch Electron Transfer for Targeted CO Photoreduction.

Construction of metal-organic cages (MOCs) with internal modifications is a promising avenue to build enzyme-like cavities and unlocking the mystery of highly catalytic activity and selectivity of enzymes. However, current interests are mainly focused on single-metal-node cages, little achievement has been expended to metallocluster-based architectures, and the in situ endogenous generation of metal clusters. Herein, based on the hard-soft-acids-bases (HSAB), the metallocluster-based heterometallic MOC (CuVMOP) constructed of [CuOPz] and [VO(OCH)(SO)(CO)] clusters was obtained by one-pot method.

Osthole ameliorates wear particle-induced osteogenic impairment by mitigating endoplasmic reticulum stress via PERK signaling cascade.

Background: Periprosthetic osteolysis and subsequent aseptic loosening are the leading causes of failure following total joint arthroplasty. Osteogenic impairment induced by wear particles is regarded as a crucial contributing factor in the development of osteolysis, with endoplasmic reticulum (ER) stress identified as a key underlying mechanism. Therefore, identifying potential therapeutic targets and agents that can regulate ER stress adaption in osteoblasts is necessary for arresting aseptic loosening.

Engineered Magneto-Piezoelectric Nanoparticles-Enhanced Scaffolds Disrupt Biofilms and Activate Oxidative Phosphorylation in Icam1 Macrophages for Infectious Bone Defect Regeneration.

Infectious bone defects pose significant clinical challenges due to persistent infection and impaired bone healing. Icam1 macrophages were identified as crucial and previously unrecognized regulators in the repair of bone defects, where impaired oxidative phosphorylation within this macrophage subset represents a significant barrier to effective bone regeneration. To address this challenge, dual-responsive iron-doped barium titanate (BFTO) nanoparticles were synthesized with magnetic and ultrasonic properties.

Senescence Reprogramming by MTHFD2 Deficiency Facilitates Tumor Progression.

Age is a critical risk factor for cancer, as its incidence and mortality increase with age. However, there is limited understanding of the molecular changes aging induces in tumors. We explored demographic differences between young and old cancer patients and identified age sixty and above as pivotal in cancer prognosis.