[Effect of seasonal distribution in precipitation on soil nitrogen mineralization in a subtropical forest].

Soil N mineralization is a key process of nutrient cycling in ecosystems. The mechanism of the seasonal distribution of precipitation on soil N mineralization remains unclear. We conducted a precipitation manipulation experiment in a subtropical forest in the middle and lower reaches of the Yangtze River in China from 2020 to 2022, with three treatments, including control (CK), decreased precipitation in the dry season with extremely increased precipitation in the wet season (T), and decreased precipitation in the dry season with proportionally increased precipitation in the wet season (T).

Germline FOXJ2 overexpression causes male infertility via aberrant autophagy activation by LAMP2A upregulation.

Spermatogenesis is a complex biological process that produces haploid spermatozoa and requires precise regulation by many tissue-specific factors. In this study, we explored the role and mechanism of Fork head box J2 (FOXJ2, which is highly expressed in spermatocytes) in the regulation of spermatogenesis using a germline-specific conditional Foxj2 knock-in mouse model (Stra8-Cre; Foxj2 mouse). Foxj2 overexpression in mouse testes led to spermatogenesis failure, which started at the initiation of meiosis, and resulted in male infertility.

[Impact of overexpressed FOXJ2 on mouse spermatogenesis and its action mechanism].

Objective: To analyze the phenotype of the male reproductive system in the germline-specific conditional Foxj2 knock-in mouse model (Stra8-cre; Foxj2tg/+), identify a target gene of the transcription factor FOXJ2, and investigate the effect of the overexpression of Foxj2 on mouse spermatogenesis and its action mechanism.

Methods: Based on the Cre-loxP recombination system, we generated a germline-specific conditional Foxj2 knock-in mouse model (Stra8-cre; Foxj2tg/+). We determined male fertility by counting the number of pups per litter and the fertilization rate after intracytoplasmic sperm injection (ICSI), observed the morphology of the testes and epididymides by HE staining, examined the sperm quality by computer assisted sperm analysis (CASA), detected the expression and localization of Cx43 in the testis by RT-qPCR, Western blot and immunohistochemistry, and verified the binding site of FOXJ2 to the Cx43 promoter using ChIP-PCR and dual luciferase reporter assay.

[Influence of snow depth changes on leaf litter decomposition of Fraxinus mandshurica and Larix gmelinii].

Changes in snowpack induced by climate change can profoundly affect forest litter decomposition. A snow depth manipulation experiment with three treatments (i.e.

[Effects of changes in seasonal snow-cover on litter decomposition and soil nitrogen dynamics in forests.].

Changes in snow-cover patterns induced by global climate change profoundly influence ecological processes in terrestrial ecosystems, including litter decomposition and soil nutrient cycling. Forest, a major terrestrial ecosystem, plays a crucial role in global biogeochemical cycling. Here, we reviewed the effects of changes in seasonal snow-cover on litter decomposition and soil nitrogen (N) cycling in forests.