Isolation and identification of the causal agent of gummy stem blight disease in Cucumis sativus caused by a bacterial pathogen in China.

Recently, a new bacterial disease was detected on cucumber stalks. In order to study the pathogenesis of this disease, the pathogenic bacteria were isolated and identified on the basis of morphological and molecular characteristics, and further analyzed for pathogenicity and antagonistic evaluation. Pathogenicity analysis showed that HlJ-3 caused melting decay and cracking in cucumber stems, and the strain reisolated from re-infected cucumber stalks was morphologically identical to HlJ-3 colonies, which is consistent with the Koch's postulates.

The highly allo-autopolyploid modern sugarcane genome and very recent allopolyploidization in Saccharum.

Modern sugarcane, a highly allo-autopolyploid organism, has a very complex genome. In the present study, the karyotype and genome architecture of modern sugarcane were investigated, resulting in a genome assembly of 97 chromosomes (8.84 Gb).

A recombinant L-threonine aldolase with high catalytic efficiency for the asymmetric synthesis of L-threo-phenylserine and L-threo-4-fluorophenylserine.

Objectives: To develop robust variants of L-threonine aldolases (L-TAs), potent catalysts for synthesizing asymmetric β-hydroxy-α-amino acids, it is necessary to identify critical residues beyond the known active site residues.

Results: Through virtual screening, a neglected residue Asn305, was identified as critical for catalytic efficiency. Subsequent site-saturation mutagenesis led to a potent variant N305R which exhibited excellent conversions of 88% (87%) and 80% (94%) for the synthesis of L-threo-phenylserine and L-threo-4-fluorophenylserine respectively.

Reciprocal links between methionine metabolism, DNA repair and therapy resistance in glioblastoma.

Glioblastoma (GBM) is uniformly lethal due to profound treatment resistance. Altered cellular metabolism is a key mediator of GBM treatment resistance. Uptake of the essential sulfur-containing amino acid methionine is drastically elevated in GBMs compared to normal cells, however, it is not known how this methionine is utilized or whether it relates to GBM treatment resistance.

Three-dimensional fluid-structure interaction modelling of the venous valve using immersed boundary/finite element method.

Research on venous hemodynamics is pivotal for unravelling venous diseases, including varicose veins and deep vein thrombosis, essential for clinical management, treatment and artificial valve design. In this study, a three-dimensional (3D) numerical simulation, employing the immersed boundary/finite element method, is constructed to explore the fluid-structure interaction (FSI) between intravenous blood and venous valves. A hyperelastic constitutive model is used to capture the incompressible, nonlinear mechanical response.

The causal effect of serum amino acids on the risk of prostate cancer: a two-sample mendelian randomization study.

Prostate cancer (PCa) is the second most common malignancy affecting men globally. Recent advances in metabolomics have highlighted significant alterations in specific amino acid (AA) metabolism linked to PCa, indicating their potential utility in diagnosis and therapy. However, no direct causal association between serum AA levels and PCa risk has been established.

Silk Fibroin-Derived N-Doped Carbon Cloth Coupling with Molybdenum Carbide Nanoparticles as a Self-Supported Electrode for Hydrogen Evolution Reaction.

Handling tons of waste textiles is a big challenge in the textile and apparel industry. The recycling of waste textiles, especially expensive silk fabrics, is of great significance for resource conservation and green, low-carbon cycle development. In addition, the development of precious metal-free electrocatalysts for the hydrogen evolution reaction (HER) remains a major challenge.

Unraveling the influence of boiling time on aroma generation in ( Maxim.) water during boiling through molecular sensory science.

water (HW) has a wide range of applications in the new food service industry. However, few process and flavor studies have been conducted on HW. The aim of this study was to investigate the effect of boiling process on HW flavor as well as the mechanisms by which important aroma compounds affect the flavor.

Immiscible metamorphic water and methane fluids preserved in carbonated eclogite.

Subduction zones metamorphic fluids are pivotal in geological events such as volcanic eruptions, seismic activity, mineralization, and the deep carbon cycle. However, the mechanisms governing carbon mobility in subduction zones remain largely unresolved. Here we present the first observations of immiscible HO-CH fluids coexisting in retrograde carbonated eclogite from the Western Tianshan subduction zone, China.

Causal Relationship Between Childhood Obesity and Sleep Apnea Syndrome: Bidirectional Two-Sample Mendelian Randomization Analysis.

Background: Childhood obesity has become a global pandemic, leading to a range of diseases. Childhood obesity appears to be associated with an increased prevalence of sleep apnea syndrome. Sleep apnea is an inestimable risk factor for thrombosis, hypertension, cardiomyopathy and many other diseases.

Negative 18F-FDG and Positive 68Ga-FAPI-04 Findings in a Patient With Acute Exacerbation of Chronic Cholecystitis.

Acute cholecystitis typically exhibits intense FDG uptake of the gallbladder wall. Radiolabeled fibroblast activation protein inhibitor (FAPI) is considered as a potential alternative agent for tumor-specific imaging. We report 18F-FDG and 68Ga-FAPI-04 PET/MR findings in a 44-year-old woman with acute exacerbation of chronic cholecystitis.

Suppresses Inflammatory Responses in Host through Its Cholesterol Metabolites.

Cholesterol is a key carbon source for (Mtb) survival and persistence within macrophages. However, little is known about the role of cholesterol metabolism by Mtb in host-Mtb interplay. Here, we report the immune suppression mediated by Mtb's cholesterol metabolites.

MiR-29b inhibits COC expansion and oocyte in vitro maturation via induction of ROS by targeting CYCS.

Cumulus-oocyte complex (COC) expansion and oocyte maturation are crucial processes for embryo development and fertility across species. Although miR-29b has been detected in porcine ovarian granulosa cells, its specific role in regulating oocyte maturation remains largely unknown. In this study, using the pig as a model, we report that over-expression of miR-29b lead to a decrease of COC expansion area and inhibits oocyte maturation (P<0.

The dual role of ATG7: Regulation of autophagy and apoptosis in porcine ovarian follicular granulosa cells.

The regulation of mammalian ovarian development involves the coordinated processes of autophagy and apoptosis. The autophagy-related gene ATG7 plays a pivotal role in mediating crosstalk between these pathways. Despite its recognized importance, the specific function of ATG7 in ovarian follicular granulosa cells remains poorly understood.

Role of TOMM34 on NF-κB activation-related hyperinflammation in severely ill patients with COVID-19 and influenza.

Background: Highly pathogenic respiratory RNA viruses such as SARS-CoV-2 and its associated syndrome COVID-19 pose a tremendous threat to the global public health. Innate immune responses to SARS-CoV-2 depend mainly upon the NF-κB-mediated inflammation. Identifying unknown host factors driving the NF-κB activation and inflammation is crucial for the development of immune intervention strategies.

Clinical performance of deep learning-enhanced ultrafast whole-body scintigraphy in patients with suspected malignancy.

Background: To evaluate the clinical performance of two deep learning methods, one utilizing real clinical pairs and the other utilizing simulated datasets, in enhancing image quality for two-dimensional (2D) fast whole-body scintigraphy (WBS).

Methods: A total of 83 patients with suspected bone metastasis were retrospectively enrolled. All patients underwent single-photon emission computed tomography (SPECT) WBS at speeds of 20 cm/min (1x), 40 cm/min (2x), and 60 cm/min (3x).

CD25FOXP3CD45RA regulatory T-cell infiltration as a prognostic biomarker for endometrial carcinoma.

Background: Regulatory T (Treg) cells reportedly play crucial roles in tumor angiogenesis as well as antitumor immunity. In order to explore their therapeutic potential, we investigated the precise prognostic impact of Treg markers in endometrial carcinoma.

Methods: We performed multiplexed immunofluorescence and quantitative image analyses of CD25, FOXP3, CTLA4, and CD45RA in tumor specimens from 176 consecutive patients treated at our institution for primary endometrial carcinomas.

Initial trajectory design of low-thrust spacecraft considering attitude constraints.

The fuel carried by deep space exploration spacecraft is crucial for the completion of their exploration missions, and the fuel for attitude control engines is even more precious. In order to reduce the control requirements for attitude control systems, this paper proposes a shape-based trajectory optimization algorithm that considers attitude constraints for low-thrust spacecraft. This method obtains a more accurate transfer trajectory by considering the change rate and change range constraints of the propulsion acceleration direction of spacecraft.

Impact of quercetin on autophagy and apoptosis induced by a high concentration of CuSO in porcine ovarian granulosa cells.

Copper is a vital micronutrient necessary for the maintenance of physiological functions. However, excessive amounts can lead to organ damage. Porcine ovarian granulosa cells are damaged by a high concentration of CuSO, which can reduce the reproductive capacity of sows.

Metabolomic Analyses Reveal That IAA from Lkbn100 Promotes Plant Defense during Infection of in Sorghum.

Global sorghum production has been significantly reduced due to the occurrence of sorghum root rot caused by the fungus . The utilization of biocontrol microorganisms has emerged as an effective strategy. However, the underlying mechanisms remain unclear.

The mouse metabolic phenotyping center (MMPC) live consortium: an NIH resource for in vivo characterization of mouse models of diabetes and obesity.

The Mouse Metabolic Phenotyping Center (MMPC)Live Program was established in 2023 by the National Institute for Diabetes, Digestive and Kidney Diseases (NIDDK) at the National Institutes of Health (NIH) to advance biomedical research by providing the scientific community with standardized, high quality phenotyping services for mouse models of diabetes and obesity. Emerging as the next iteration of the MMPC Program which served the biomedical research community for 20 years (2001-2021), MMPCLive is designed as an outwardly-facing consortium of service cores that collaborate to provide reduced-cost consultation and metabolic, physiologic, and behavioral phenotyping tests on live mice for U.S.