Per- and polyfluoroalkyl substances exacerbate the prevalence of plasmid-borne antibiotic resistance genes by enhancing natural transformation,in vivo stability, and expression in bacteria.

Per- and polyfluoroalkyl substances (PFAS) as emerging pollutants are ubiquitous and disrupt biological processes across water boundaries. Their coexistence with antibiotic resistance genes (ARGs) in water matrix is associated with the spread of ARGs via conjugative transfer, posing a threat to public health. However, their role in natural transformation-where microorganisms actively take up extracellular ARGs (eARGs)-and the subsequent persistence and expression of ARGs after transformation remains poorly understood.

From technique mastery to scientific thinking: how open-ended experiments shape medical students' perception of laboratory course roles in China.

Background: Basic science education is universally acknowledged as a pivotal component of medical education. In China, medical students undergo significant preclinical training but often lack scientific thinking. As China transitions to a knowledge-intensive nation, addressing this gap is crucial.

Spatio-Temporal Heterogeneity of Ecological Quality in a Typical Dryland of Northern China Driven by Climate Change and Human Activities.

With the intensification of climate change and anthropogenic impacts, the ecological environment in drylands faces serious challenges, underscoring the necessity for regionally adapted ecological quality evaluation. This study evaluates the suitability of the original Remote Sensing Ecological Index (oRSEI), modified RSEI (mRSEI), and adapted RSEI (aRSEI) in a typical dryland region of northern China. Spatio-temporal changes in ecological quality from 2000 to 2022 were analyzed using Theil-Sen median trend analysis, the Mann-Kendall test, and the Hurst exponent.

Pharmacokinetics, pharmacodynamics and bioavailability of dexmedetomidine nasal spray in healthy Chinese adults: A phase I clinical trial.

Background: Intranasal administration is a convenient route for drug delivery that can be applied for procedural sedation. However, there is currently limited exploration into fixed dosing regimens. This study was to investigate the pharmacokinetics (PK), pharmacodynamics (PD), bioavailability (BA) and safety of dexmedetomidine after fixed doses of intranasal and intravenous administration in healthy male and female subjects.

Highly Oriented and Ordered Co-Assembly Monolayers for Inverted Perovskite Solar Cells.

Perovskite solar cells with inverted architecture have remarkable power conversion efficiency (PCE) and operating stability based on self-assembled molecules (SAMs) hole transport layer. Homogeneous distribution and covalent binding mode of SAM monolayers are critical to improving interfacial property and reducing interfacial losses, which can be achieved through molecular design and co-assembly strategy. Here, we propose co-assembly strategy with SAM by employing a novel 2D π-conjugated structure graphdiyne derivative (PAG) with phosphoric acid groups.

Causal relationship between physical activity and scoliosis: A Mendelian randomization study.

Scoliosis, marked by abnormal spinal curvature, is common in adolescents and can lead to chronic pain and reduced quality of life. The relationship between physical activity and scoliosis is debated. In this study, we aim to investigate the causal relationship between physical activity levels and idiopathic scoliosis risk using the Mendelian randomization (MR) approach.

Comparison of Back Tuina and Oral Astragalus Methods to Enhance Immunosuppression in Rabbits and Prediction of Astragalus Targets for Immune Function Improvement.

Back tuina massage and oral administration of astragalus are two methods used in traditional Chinese medicine (TCM) to improve immune function. The aim of this study is to analyze the effects of tuina and orally administered astragalus on immunosuppressed rabbits using proteomics technology. Additionally, we investigate the potential mechanism of action of astragalus components on immunosuppression using an integration of network pharmacology and molecular docking technology.

Epigallocatechin Gallate Combine with Ice Glazing: A Promising Way to Preserve the Quality of Frozen .

The quality of frozen plays an important role in influencing consumer preference. Polyphenol oxidase (PPO) activity changes are commonly used to evaluate melanosis in aquatic products. In this study, we examined the interactions between epigallocatechin gallate (EGCG) and PPO.

Optimized Liquid Medium Formulation for and Biological Activity of the Exopolysaccharides.

Aims: () is a rare medicinal mushroom that is rich in polysaccharides, triterpenes, flavonoids, and other bioactive compounds. It has good potential development value.

Methods And Results: We performed single factor experiments and Box-Behnken response surface methodology to optimize the liquid fermentation medium formulation for with mycelial biomass as the indicator.

Porous Haldane model: topological phase transitions and flat bands.

To investigate the influence of nanoholes on Chern insulators (CIs), we propose a porous Haldane model that considers the nearest-neighbor (NN) hoppings and next-NN (NNN) hoppings with staggered magnetic fluxes. This model supports multiple topological phases with different filling factors. At 2/5 filling, CI phases withC=±1, = 2,C=±3,C=±4and higher-order topological insulator (HOTI) appear.

The predictive value of pan-immune inflammatory index for early recurrence of atrial fibrillation after cryoablation.

Objective: The pan-immune inflammatory (PIV) index holds prognostic value for cardiovascular diseases. This study aimed to investigate the predictive value of the PIV index regarding recurrence of atrial fibrillation (AF) after cryoballoon ablation (CBA).

Methods: The study included 307 patients with AF.

Boosting pulse characterization precision for Ho: YAP double pulse laser with back propagation neural network.

The precise energy and temporal control advantages of the 2 µm double-pulse laser have diverse applications in laser processing, biomedicine, and communications. The Ho: YAP Q-switched double pulse laser, a complex system, demands comprehensive theoretical analysis and precise experimental operations, especially when managing pulse overlap and ensuring output stability. Traditional design methods, time-consuming and labor-intensive, pose challenges in error elimination and susceptibility to environmental and device instabilities.

Proteomic Signature of Body Mass Index and Risk of Type 2 Diabetes.

The obesity diagnosis by body mass index (BMI) exhibits considerable interindividual heterogeneity in metabolic phenotypes and risk of developing type 2 diabetes (T2D). We investigated the association of proteomic signature of BMI and T2D and examined whether the proteomic signature of BMI improves prediction of T2D risk. This study included 41,427 adults in the UK Biobank who were free of T2D at baseline and had complete data on proteomics metrics assessed by antibody based Olink assay.

Plasma proteomics-based brain aging signature and incident dementia risk.

Investigating brain-enriched proteins with machine learning methods may enable a brain-specific understanding of brain aging and provide insights into the molecular mechanisms and pathological pathways of dementia. The study aims to analyze associations of brain-specific plasma proteomic aging signature with risks of incident dementia. In 45,429 dementia-free UK Biobank participants at baseline, we generated a brain-specific biological age using 63 brain-enriched plasma proteins with machine learning methods.

Enhancing SO Sensing Performance of a Fuel Cell-Type Sensor with N-Doped Cu/CNT Aerogel Electrode and COF/Nafion Electrolyte.

Sulfur dioxide (SO) is a common environmental pollutant with significant hazards. However, sensors for SO real-time monitoring at room temperature often face problems such as a poor response and sluggish recovery. In this work, a fuel cell-type gas sensor based on nitrogen-doped carbon nanotube (CNT) aerogels loaded with Cu particle electrode material and COF/Nafion composite electrolyte was developed, which exhibited excellent SO sensitivity and fast response/recovery.

Electrochemical splitting of methane in melts: Producing and tuning high-value carbon materials with controllable morphology.

Catalytic decomposition of methane offers a viable solution for producing pure hydrogen and nanocarbon without emitting carbon dioxide. However, conventional thermal catalytic processes and catalysts have limitations in terms of poor carbon quality and catalyst deactivation due to carbon deposition. The newly developed electrochemical splitting of methane (ESM) in molten salt has emerged as a promising alternative that allows for the separate production of hydrogen at the anode and carbon deposition at the cathode.

Protective effect of water-soluble nervonic acid micro-powder coated with chitosan oligosaccharide and silk fibroin on hippocampal neuronal HT22 cells.

Nervonic acid (NA) is an extremely long chain monounsaturated fatty acid that plays a crucial biological role in brain development and repair. However, its low solubility reduced bioavailability and limited its applications. In this study, spherical water-soluble nervonic acid composite micro-powder (NA-WM) was constructed by layer-by-layer self-assembly technology under electrostatic interaction and hydrogen bond, in which electronegative NA was used as the core material, and electropositive COS (Chitosan oligosaccharide) with neuroprotective properties and electronegative SF (Silk fibroin) with biocompatibility and anti-inflammatory synergism were used as the wall material.

Ultra-processed food consumption, plasma metabolite profile, and risk of all-cause and cause-specific mortality in a population-based cohort.

Background & Aims: Epidemiological evidence on ultra-processed food (UPF) and cause-specific mortality remains limited and mixed. Molecular mechanisms underlying UPF intake and mortality remain unexplored. This study aimed to evaluate the associations between UPF consumption, metabolic signatures, and all-cause, premature, and cause-specific mortality.

Sports-ACtrans Net: research on multimodal robotic sports action recognition driven via ST-GCN.

Introduction: Accurately recognizing and understanding human motion actions presents a key challenge in the development of intelligent sports robots. Traditional methods often encounter significant drawbacks, such as high computational resource requirements and suboptimal real-time performance. To address these limitations, this study proposes a novel approach called Sports-ACtrans Net.