Targeted Depletion of Individual Pathogen by Bacteria-Templated Polymer.

Selective and targeted removal of individual species or strains of bacteria from complex communities can be desirable over traditional and broadly acting antibiotics in several conditions. However, strategies that can detect and ablate bacteria with high specificity are emerging in recent years. Herein, a platform is reported that uses bacteria as a template to synthesize polymers containing guanidinium groups for self-selective depletion of specific pathogenic bacteria without disturbing microbial communities.

Glass Nanopipette-Based Plasmonic SERS Platform for Single-Cell MicroRNA-21 Sensing during Apoptosis.

As one of the most widely distributed microRNAs, microRNA-21 (miRNA-21) significantly regulates target genes' expression levels and participates in many cellular and intercellular activities, and its abnormal expression is always related to some diseases, especially cancer. Hence, detecting miRNA-21, as a biomarker, at the single-cell level helps us to reveal cell heterogeneity and expression level variation during the state change of cells. In this study, we constructed a gold nanoparticles nanomembrane (AuNPs-NM)-modified plasmonic glass nanopipette (P-nanopipette) surface-enhanced Raman scattering (SERS) sensing platform to sensitively detect content variation of the intracellular miRNA-21 during the electrostimulus (ES)-induced apoptosis process.

Temporal-spatial Generation of Astrocytes in the Developing Diencephalon.

Astrocytes are the largest glial population in the mammalian brain. However, we have a minimal understanding of astrocyte development, especially fate specification in different regions of the brain. Through lineage tracing of the progenitors of the third ventricle (3V) wall via in-utero electroporation in the embryonic mouse brain, we show the fate specification and migration pattern of astrocytes derived from radial glia along the 3V wall.

Oil-Soluble Exogenous Catalysts and Reservoir Minerals Synergistically Catalyze the Aquathermolysis of Heavy Oil.

Oil is the "blood" and economic lifeblood of modern industry, but traditional light crude oil has been over-consumed, and it has been difficult to meet human demand for energy, so the exploitation of heavy oil is particularly important. In this paper, an oil-soluble catalyst was synthesized to catalyze the pyrolysis reaction of heavy oil in collaboration with reservoir minerals, so as to achieve efficient viscosity reduction of heavy oil and reduce production costs. The experimental results showed that Zn(II)O + K had the best synergistic viscosity reduction effect after the aquathermolysis of No.

Epidemical trends and risk factors of PTSD in parents of critically ill children: Evidence from both meta-analysis and subgroup analysis.

Background: Post-Traumatic Stress Disorder (PTSD) in parents traumatized by a child's critical illness, while differences in prevalence and risk factors for parental PTSD in previous studies. This study aimed to assess trends in the prevalence and risk factors for PTSD in parents of critically ill children.

Methods: Nine main databases were searched to extract the prevalence, sample size and risk factors from the published literature.

The potential DNA methylation markers of cardiovascular disease in patients with type 2 diabetes.

Background: DNA methylation is associated with cardiovascular (CV) disease. However, in type 2 diabetes (T2D) patients, the role of gene methylation in the development of CV disease is under-studied. We aimed to identify the CV disease-related DNA methylation loci in patients with T2D and to explore the potential pathways underlying the development of CV disease using a two-stage design.

Single-cell allele-specific expression analysis reveals dynamic and cell-type-specific regulatory effects.

Differential allele-specific expression (ASE) is a powerful tool to study context-specific cis-regulation of gene expression. Such effects can reflect the interaction between genetic or epigenetic factors and a measured context or condition. Single-cell RNA sequencing (scRNA-seq) allows the measurement of ASE at individual-cell resolution, but there is a lack of statistical methods to analyze such data.

A Bayesian Federated Learning Framework With Online Laplace Approximation.

Federated learning (FL) allows multiple clients to collaboratively learn a globally shared model through cycles of model aggregation and local model training, without the need to share data. Most existing FL methods train local models separately on different clients, and then simply average their parameters to obtain a centralized model on the server side. However, these approaches generally suffer from large aggregation errors and severe local forgetting, which are particularly bad in heterogeneous data settings.

Icaritin ameliorates RANKL-mediated osteoclastogenesis and ovariectomy-induced osteoporosis.

A rapidly aging society and longer life expectancy are causing osteoporosis to become a global epidemic. Over the last five decades, a number of drugs aimed at reducing bone resorption or restoring bone mass have been developed, but their efficacy and safety are limited. Icaritin (ICT) is a natural compound extracted from anti-osteoporosis herb spp.

Genome-Wide Analysis of the Amino Acid Permeases Gene Family in Wheat and Enhanced Salt Tolerance by Accumulating Ethylene.

Amino acid permeases (AAPs) are proteins of the integral membrane that play important roles in plant growth, development, and responses to various stresses. The molecular functions of several AAPs were characterized in and rice, but there is still limited information on wheat. Here, we identified 51 genes () in the wheat genome, classified into six groups based on phylogenetic and protein structures.

Binding-induced fibrillogenesis peptide inhibits RANKL-mediated osteoclast activation against osteoporosis.

Osteoporosis is primarily driven by an imbalance between bone resorption and formation, stemming from enhanced osteoclast activity during bone remodeling. At the crux of this mechanism lies the pivotal RANK-RANKL-OPG axis. In our study, we designed two binding-induced fibrillogenesis (BIF) peptides, namely BIFP and BIFY, targeting RANK and RANKL, respectively.

Calcium signaling-mediated transcriptional reprogramming during abiotic stress response in plants.

Calcium (Ca) is a second messenger in plants growth and development, as well as in stress responses. The transient elevation in cytosolic Ca concentration have been reported to be involved in plants response to abiotic and biotic stresses. In plants, Ca-induced transcriptional changes trigger molecular mechanisms by which plants adapt and respond to environment stresses.

Construction of Low-Cost Z-Scheme Heterojunction CuO/PCN-250 Photocatalysts Simultaneously for the Enhanced Photoreduction of CO to Alcohols and Photooxidation of Water.

Solar-driven high-efficiency conversion of CO with water vapor into high-value-added alcohols is a promising approach for reducing CO emissions and achieving carbon neutrality. However, the rapid recombination of photogenerated carriers and low CO adsorption capacity of photocatalysts are usually the factors that limit their applicability. Herein, a series of low-cost Z-scheme heterostructures CuO/PCN-250- are constructed by growth of ultrasmall CuO nanoparticles on PCN-250.

Exploring the Robustness of Human Parsers Toward Common Corruptions.

Human parsing aims to segment each pixel of the human image with fine-grained semantic categories. However, current human parsers trained with clean data are easily confused by numerous image corruptions such as blur and noise. To improve the robustness of human parsers, in this paper, we construct three corruption robustness benchmarks, termed LIP-C, ATR-C, and Pascal-Person-Part-C, to assist us in evaluating the risk tolerance of human parsing models.

KNOTTED1-like homeobox (KNOX) transcription factors - Hubs in a plethora of networks: A review.

KNOX (KNOTTED1-like HOMEOBOX) belongs to a class of important homeobox genes, which encode the homeodomain proteins binding to the specific element of target genes, and widely participate in plant development. Advancements in genetics and molecular biology research generate a large amount of information about KNOX genes in model and non-model plants, and their functions in different developmental backgrounds are gradually becoming clear. In this review, we summarize the known and presumed functions of the KNOX gene in plants, focusing on horticultural plants and crops.

An explainable machine-learning approach for revealing the complex synthesis path-property relationships of nanomaterials.

Machine learning (ML) models have recently shown important advantages in predicting nanomaterial properties, which avoids many trial-and-error explorations. However, complex variables that control the formation of nanomaterials exhibiting the desired properties still need to be better understood owing to the low interpretability of ML models and the lack of detailed mechanism information on nanomaterial properties. In this study, we developed a methodology for accurately predicting multiple synthesis parameter-property relationships of nanomaterials to improve the interpretability of the nanomaterial property mechanism.

Evaluation of polymyxin B AUC/MIC ratio for dose optimization in patients with carbapenem-resistant infection.

Polymyxin B has been used as a last-line therapy for the treatment of carbapenem-resistant gram-negative bacterial infection. The pharmacokinetic/pharmacodynamic index (AUC/MIC) of polymyxin B has not been clinically evaluated, given that the broth microdilution method for polymyxin susceptibility testing is rarely used in hospitals. This study analyzed data from 77 patients with carbapenem-resistant infections.

Efficient capture and highly sensitive analysis of okadaic acid by three-dimensional covalent organic frameworks with hydroxyl surface engineering.

A novel three-dimensional covalent organic framework (3D-COF) with content-tunable and active hydroxyl groups (OH) on the pore walls was developed and adopted for the high-performance capture of okadaic acid (OA) marine toxins. Using pore-surface engineering, the integration of linear building blocks (4,4'-diamino-3,3'-biphenyldiol, BD(OH) and benzidine, BD) with the 3D structural building block backbone (4,4',4'',4'''-methane-tetrayltetrabenzaldehyde, TFPM) was achieved. By adjusting the ratio of BD(OH), functional multicomponent-COFs [OH]-BD-TFPM COFs (X = 25%) were synthesized, which offered ideal access to convert a conventional COF into a functional platform with multiple-mode interactions of hydrophobic and hydrophilic groups for OA capture.

Molecular mechanisms of low-temperature sensitivity in tropical/subtropical plants: a case study of .

Low temperature is a limiting factor affecting plant growth and development. , a typical tropical and subtropical tree important for the ecological restoration of coastal beaches, is sensitive to cold stress. By comparing cold tolerance between and , we investigated the molecular basis underlying the cold sensitivity of .

Three dimensional high-performance micro-supercapacitors with switchable high power density and high energy density.

In the field of microscale energy storage, the fabrication of micro-supercapacitors (MSCs) with high power density and high energy density has always been a focus of research. In this work, laser-induced porous graphene and chemically deposited manganese dioxide nanoparticles are used as electrode materials, and a switchable MSC with two energy storage principles is obtained by designing symmetric interdigitated and square electrode structures. The aim is to overcome the preparation challenge of supercapacitors with high energy density and high power density by switching between two modes.

Ultrasound-mediated multifunctional magnetic microbubbles for drug delivery of celastrol in VX2 liver transplant tumors.

Celastrol (CST) has positive pharmacological effects on various cancers, but clinical application is limited because of poor water solubility and systemic toxicity. Ferric oxide (FeO) has a large specific surface area and can be functionalized by inorganic modification to form complex magnetic drug delivery systems. Herein, FeO was surface-modified with citric acid and polyethylene glycol (PEG) (via) the Mitsunobu reaction and then covalently bound to CST.

cRGD-Conjugated GdIO Nanoclusters for the Theranostics of Pancreatic Cancer through the Combination of T-T Dual-Modal MRI and DTX Delivery.

Clinically, magnetic resonance imaging (MRI) often uses contrast agents (CAs) to improve image contrast, but single-signal MRI CAs are often susceptible to calcification, hemorrhage, and magnetic sensitivity. Herein, iron acetylacetone and gadolinium acetylacetone were used as raw materials to synthesize a T-T dual-mode imaging gadolinium-doped iron oxide (GdIO) nanocluster. Moreover, to endow the nanoclusters with targeting properties and achieve antitumor effects, the cyclic Arg-Gly-Asp (cRGD) peptide and docetaxel (DTX) were attached to the nanocluster surface, and the efficacy of the decorated nanoclusters against pancreatic cancer was evaluated.