Reoviruses infect many mammals and are widely studied as a model system for enteric viruses. However, most of our reovirus knowledge comes from laboratory strains maintained on immortalized L929 cells. Herein, we asked whether naturally circulating reoviruses possess the same genetic and phenotypic characteristics as laboratory strains.
View Article and Find Full Text PDFMammalian orthoreovirus serotype 3 Dearing is an oncolytic virus currently undergoing multiple clinical trials as a potential cancer therapy. Previous clinical trials have emphasized the importance of prescreening patients for prognostic markers to improve therapeutic success. However, only generic cancer markers such as epidermal growth factor receptor (EGFR), Hras, Kras, Nras, Braf, and p53 are currently utilized, with limited benefit in predicting therapeutic efficacy.
View Article and Find Full Text PDFRNAs with methylated cap structures are present throughout multiple domains of life. Given that cap structures play a myriad of important roles beyond translation, such as stability and immune recognition, it is not surprising that viruses have adopted RNA capping processes for their own benefit throughout co-evolution with their hosts. In fact, that RNAs are capped was first discovered in a member of the family, , before these findings were translated to other domains of life.
View Article and Find Full Text PDFVirion assembly is an important step in the life cycle of all viruses. For viruses of the Flavivirus genus, a group of enveloped positive-sense RNA viruses, the assembly step represents one of the least understood processes in the viral life cycle. While assembly is primarily driven by the viral structural proteins, recent studies suggest that several nonstructural proteins also play key roles in coordinating the assembly and packaging of the viral genome.
View Article and Find Full Text PDFThe aim of this study was to determine the effect of varying excipient content on the formation and physical properties of 3 D printed tablets. Fifteen different excipient preparations were formed into tablets with radii of 5 mm and thickness of 2 mm, using binder jetting (BJ). The tablets were analyzed by assessing visual and microstructural appearance, friability, hardness, and disintegration time.
View Article and Find Full Text PDFDrug Dev Ind Pharm
December 2018
Individualized medicine is a new direction in the field of modern pharmacy. In this study, we assessed the feasibility and accuracy of 3D printing techniques for the preparation of individualized doses of mouth-disintegrating tablets of warfarin. Warfarin sodium, D-sucrose, pregelatinized starch, povidone K30, microcrystalline cellulose, and silicon dioxide (at a ratio of 1:42.
View Article and Find Full Text PDFShi Yan Sheng Wu Xue Bao
June 2003
Two cDNA fragments, named for SRGKC2 and SRGKC3, encoding cyclophilin in Kandelia candel were isolated by Representational Difference Analysis of cDNA. The two cDNA fragments were 282 bp and 160 bp, respectively. Sequence analysis shows that both of the SRGKC2 and SRGKC3 come from the same gene region, and SRGKC3 is a part of SRGKC2.
View Article and Find Full Text PDFIdentifying changes in gene expression is of prime interest in molecular biology. A variety of methods have been developed for analyzing differentially expressed genes. Among these techniques, differential display reverse transcription PCR (DDRT-PCR), representational difference analysis (RDA) and suppression subtractive hybridization (SSH), which were based on differentially screening and subtractive hybridization technology, are applied most widely.
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