Influence of Heat- and Cold-Stressed Raw Milk on the Stability of UHT Milk.

This study investigated the variations and alterations in the concentrations of plasmin system components in raw and UHT (ultra-high-temperature) milk under cold stress (WCT ≤ -25 °C), heat stress (THI ≥ 80), and normal (THI < 70 and WCT ≥ -10 °C) circumstances. The findings indicated elevated amounts of plasmin system components in cold-stressed raw milk. While storing UHT milk at 25 °C, the concentrations and activity of plasmin in the milk exhibited an initial increase followed by a decrease, peaking around the 30th day.

Bioinformatics and Expression Profiling of the DHHC-CRD S-Acyltransferases Reveal Their Roles in Growth and Stress Response in Woodland Strawberry ().

Protein S-acyl transferases (PATs) are a family of enzymes that catalyze protein S-acylation, a post-translational lipid modification involved in protein membrane targeting, trafficking, stability, and protein-protein interaction. S-acylation plays important roles in plant growth, development, and stress responses. Here, we report the genome-wide analysis of the family genes in the woodland strawberry (), a model plant for studying the economically important Rosaceae family.

ERBB3-related gene PBX1 is associated with prognosis in patients with HER2-positive breast cancer.

Background: HER2-positive breast cancer (BC) is a subtype of breast cancer. Increased ERBB3 expression has been implicated as a potential cause of resistance to other HER-targeted therapies. Our study aimed to screen and validate prognostic markers associated with ERBB3 expression by bioinformatics and affecting the prognosis of HER2 staging.

Co-application of polyethylene oxide (PEO), biochar, and seaweed fertilizer improves desert soil properties.

Improving water retention, erosion resistance and nutrients in desert areas is essential for ecological sustainability. This study evaluated the effects of biochar, polyethylene oxide (PEO), and seaweed fertilizer on the properties of desert sandy soil, focusing on water retention, erosion resistance, and soil nutrients. The sandy soil used in the study was taken from the Tengger Desert in Gansu, China, and an orthogonal experimental design was used to select three different proportions of biochar, PEO, and seaweed fertilizer.

Heparan sulfate acts in synergy with tight junction through STAT3 signaling to maintain the endothelial barrier and prevent lung injury development.

Damage to glycocalyx and tight junction are key determinants of endothelial permeability, which is the main pathological feature of acute respiratory distress syndrome (ARDS). However, the effect of glycocalyx heparan sulfate (HS) on tight junction proteins occludin and ZO-1 has not been revealed. In this study, the mice exposed to LPS results showed that FITC-albumin infiltration, HS shedding, and tight junction protein impairment were most severe at 6 h of LPS treatment compared with those in other treatment times.

Machine Learning for Automated Identification of Anatomical Landmarks in Ultrasound Periodontal Imaging.

Objectives: To identify landmarks in ultrasound periodontal images and automate the image-based measurements of gingival recession (iGR), gingival height (iGH), and alveolar bone level (iABL) using machine learning.

Methods: We imaged 184 teeth from 29 human subjects. The dataset included 1580 frames for training and validating the U-Net CNN machine learning model, and 250 frames from new teeth that were not used in training for testing the generalization performance.

Metagenomic Insight into the Associated Microbiome in Plasmodia of Myxomycetes.

During the trophic period of myxomycetes, the plasmodia of myxomycetes can perform crawling feeding and phagocytosis of bacteria, fungi, and organic matter. Culture-based studies have suggested that plasmodia are associated with one or several species of bacteria; however, by amplicon sequencing, it was shown that up to 31-52 bacteria species could be detected in one myxomycete, suggesting that the bacterial diversity associated with myxomycetes was likely to be underestimated. To fill this gap and characterize myxomycetes' microbiota and functional traits, the diversity and functional characteristics of microbiota associated with the plasmodia of six myxomycetes species were investigated by metagenomic sequencing.

[Next-generation sequencing-based minimal residual disease detection reveals clonal evolution in pediatric acute B-lymphoblastic leukemia: a case report and literature review].

Minimal residual disease (MRD), a crucial biomarker for assessing efficacy and predicting recurrence, refers to residual tumor cells remaining in the body of patients with hematological malignancies who achieved complete remission after treatment. This study aimed to conduct a retrospective analysis of the clinical diagnosis, treatment, and MRD monitoring of a pediatric patient with multiple acute B-lymphocytic leukemia relapses, alongside a review of relevant literature. In this case, Ig rearrangement based on next-generation sequencing (NGS) was more accurate in assessing the MRD level, compared with the traditional method of MRD detection, indicating the risk of earlier relapse and guided interventions in time.

[Mitoxantrone hydrochloride liposome combined with cytarabine for treating pediatric acute myeloid leukemia with RUNX1∷MTG16 fusion gene: a case report and literature review].

This case report presents a patient with pediatric acute myeloid leukemia (AML) with RUNX1∷MTG16, admitted to the Blood Disease Hospital of the Chinese Academy of Medical Sciences in October 2023. He was 13 years old, with a chief complaint of fatigue for 20 days. Bone marrow smear revealed 17.

Elbow rotation affects the accuracy of rotational formulas: validation of a modified method.

Background: Supracondylar humerus fractures (SCHFs) are the most common elbow fractures in children and are typically treated with closed reduction and Kirschner pin fixation. However, varying degrees of residual rotational displacement may remain after closed reduction. Several methods exist to assess rotational displacement, but none account for the effect of elbow rotation on the results.

Effects of low-volume court-based sprint interval training on aerobic capacity and sport-specific endurance performance in competitive tennis players.

Sprint interval training (SIT) is a potent exercise strategy to enhance athletes' aerobic capacity in a time-efficient manner. The purpose of this study was to examine the effect of a low-volume court-based SIT program on aerobic capacity and sport-specific endurance performance for competitive tennis players. Sixteen competitive collegiate tennis players were randomly assigned to the SIT (court-based repeated-sprint training) and traditional endurance training (ET; 45-min continuous treadmill running) groups for a 6-week intervention (3 sessions/week).

Preparation, structural characterisation, absorption and calcium transport studies of walnut peptide calcium chelate.

In this study, a walnut peptide (WP) with calcium-binding capacity was prepared using a combination of alkalase and neutrase. The conditions for the preparation of walnut peptide calcium chelate (WP-Ca) were optimised (a peptide/calcium chloride ratio of 1 : 4 for 70 min at 50 °C and pH 9.5).

Design and test of steam-injected continuous scrambled egg device.

To solve the existing problems of low yield, uneven quality, and single form of industrially scrambled eggs, we have developed a continuous high-output steam scrambled egg device based on the principle of steam injected. By establishing calibration curves for egg, oil, and steam flow rates, determining the key parameters of the equipment, and simulating the heat transfer process between steam and egg by Computational Fluid Dynamics (CFD), we created the device and verified its production performance. The results show that the capacity of this device can reach 104.

Thermal aggregation and gelation behaviors of glucono-δ-lactone-induced soy protein hydrolysate gels: Effects of protein and coagulant concentrations.

In this study, a novel acid-induced heat-set soy protein hydrolysate (SPH) gel was successfully developed. The effects of protein (7 and 8 wt%) and glucono-δ-lactone (GDL, 4, 6, 8, and 10 wt%) concentrations on its aggregation and gelation behaviors were investigated by evaluating the structural, rheological, textural, and physical properties of the SPH gel. The structural properties revealed that GDL promoted the formation of SPH aggregates and gels, primarily via disulfide bonds and hydrophobic interactions, which were closely related to the unfolding of the protein structure, exposed hydrophobic groups, decreased protein solubility, and increased particle size and turbidity during the heating process.

Advancing Phage Therapy: A Comprehensive Review of the Safety, Efficacy, and Future Prospects for the Targeted Treatment of Bacterial Infections.

Background: Phage therapy, a treatment utilizing bacteriophages to combat bacterial infections, is gaining attention as a promising alternative to antibiotics, particularly for managing antibiotic-resistant bacteria. This study aims to provide a comprehensive review of phage therapy by examining its safety, efficacy, influencing factors, future prospects, and regulatory considerations. The study also seeks to identify strategies for optimizing its application and to propose a systematic framework for its clinical implementation.

Morin Improves the Bone Histomorphology and Biochemical Markers in an Animal Model of Ovariectomy-Induced Osteoporosis by Suppressing Autophagy and Apoptosis.

Osteoporosis (OP) is the most prevalent metabolic bone disease and an important postmenopausal consequence. This study aimed to investigate the effects of morin, a flavonoid with beneficial properties, on ovariectomy-induced OP. Animals were ovariectomized (OVX) and treated with different doses of morin (15, 30, and 45 mg/kg/day) or estradiol (10 μg/kg/day) for 10 weeks by gavage.

Yield increment and transcriptome response caused by blue light treatment in Hericium coralloides.

Background: Hericium coralloides is a traditional edible and medicinal mushroom. Light is a key factor in forming fruiting bodies of fungi; however, the effects of different light on the yield and morphogenesis of H. coralloides are still unknown.

KLHL24 associated cardiomyopathy: Gene function to clinical management.

Background: KLHL24 (Kelch-like protein 24) is a significant component of the ubiquitin-proteasome system (UPS), involved in regulating protein turnover through targeted ubiquitination and degradation. Germline mutations in KLHL24 gene have been known to cause Epidermolysis Bullosa Simplex characterized by skin fragility but has recently been found to cause Cardiomyopathy.

Main Body: Various cardiomyopathies, including hypertrophic cardiomyopathy and dilated cardiomyopathy, leading to abnormal protein degradation and affecting the stability and function of essential cardiac proteins which finally results into structural and functional abnormalities in cardiac muscle.

Effects of pH shift and D-galactose on network structure of glycinin gel and diffusion behavior of non-network proteins.

To reduce the content of non-network proteins in protein-based gels, a synergistic approach involving pH shift and D-galactose (DG) was developed herein to obtain elastic gels with dense networks. The results revealed that the combined effect of pH shift and DG promoted the formation of additional disulfide bonds and chemical bonds between molecules, resulting in a denser, and highly elastic gel network, which immobilized more aggregates, leading to a significant reduction in non-network protein content, and enhancing the functional properties of the gel. Moreover, non-network proteins primarily consisted of subunit A (mostly Glu and Asp), while the subunit B was the primary polypeptide forming the gel network.

[Strategies for prevention and treatment of spinal degenerative diseases from perspective of traditional Chinese medicine for bone health].

The population aging and the coming of the information era are accompanied with the growing incidence of spinal degenerative diseases, which result in heavy social and economic burdens. Under the guidance of the tendon-bone theory, rich experience has been accumulated in the prevention and diagnosis of spinal degenerative diseases with traditional Chinese medicine(TCM), which demonstrates unique advantages. China's government has placed people's health in the strategic position of development, providing a favorable environment for the realization of healthy aging.

Development and Validation of an Explainable Prediction Model for Postoperative Recurrence in Pediatric Chronic Rhinosinusitis.

Objective: This study aims to develop an interpretable machine learning (ML) predictive model to assess its efficacy in predicting postoperative recurrence in pediatric chronic rhinosinusitis (CRS).

Study Design: A decision analysis was performed with retrospective clinical data.

Setting: Recurrent group and nonrecurrent group.

Operational Police Stress Is Associated With Disordered Eating in Police Officers.

Objective: Police officers are under high operational stress and are at high risk for stress-related mental health disturbances. However, little research has investigated disordered eating in this population. In this study, we compared disordered eating symptoms between male and female police officers, examined the association between operational police stress and disordered eating symptoms, and explored body mass index (BMI) and sex as potential moderators of these associations.