Co-metabolism of Norfloxacin by Chlorella pyrenoidosa: Carbon source effects, biotransformation mechanisms, and key driving genes.

Co-metabolism with appropriate carbon sources has been demonstrated to effectively enhance the removal of ubiquitous recalcitrant micropollutant by microalgae. However, the specific impacts of carbon sources on the co-metabolism of antibiotics by microalgae remain insufficiently explored. In this study, transcriptomics, gene network analysis, extracellular polymeric substances (EPS), and enzymatic activity involved in co-metabolic pathways of norfloxacin (NFX), were systematically evaluated to investigate the underlying biological mechanisms involved in NFX co-metabolism by Chlorella pyrenoidosa.

Evaluation of innovative drying technologies in drying considering product quality and drying efficiency.

is widely used as healthy food and herbal medicine for its anti-inflammatory, analgesic, antihypertensive, and antiviral functions. The drying behavior and physicochemical quality of were studied to evaluate its adaptability under four drying techniques: hot air drying (HAD), medium-and short-wave infrared drying (MSWID), pulsed vacuum drying (PVD), and radio frequency-HAD (RF-HAD). Compared with HAD and MSWID, PVD and RF-HAD can form beneficial microporous channels for moisture migration inside , thus shortening drying time by 32.

CRISPR/Cas12a-Enabled Amplification-Free Colorimetric Visual Sensing Strategy for Point-of-Care Diagnostics of Biomarkers.

CRISPR/Cas12a-based biosensors have garnered significant attention in the field of point-of-care testing (POCT), yet the majority of the CRISPR-based POCT methods employ fluorescent systems as report probes. Herein, we report a new CRISPR/Cas12a-enabled multicolor visual biosensing strategy for the rapid detection of disease biomarkers. The proposed assay provided vivid color responses to enhance the accuracy of visual detection.

Exogenous Trilobatin Enhances Flavonoid Content in Purple Rice Grains and Affects the Flavonoid Biosynthesis Pathway.

Antioxidant activity and flavonoid content are important characteristics of colored rice grains. Previously, we obtained a preliminary understanding of the metabolic markers of antioxidant activity, namely, phlorizin and trilobatin, in different colored rice varieties and purple rice grains at different growth stages, but the mechanisms associated with these markers have not yet been confirmed. In this study, purple rice was selected as the experimental material, and clover extract was applied during the grain-filling stage to explore the impact of clover extract on the total antioxidant capacity and flavonoid biosynthesis in purple rice grains.